Alpha‐Synuclein Oligomers Driven T1–T2 Switchable Nanoprobes for Early and Accurate Diagnosis of Parkinson's Disease

Abstract

The alpha‐synuclein (α‐syn) oligomers hold a central role in the pathology of Parkinson's disease (PD). Achieving accurate detection of α‐syn oligomers in vivo presents a promising avenue for early and accurate diagnosis of PD. Magnetic resonance imaging (MRI), with non‐invasion and exceptional tissue penetration, offers a potent tool for visualizing α‐syn oligomers in vivo. Nonetheless, ensuring diagnostic specificity remains a formidable challenge. Herein, a novel MRI probe (ASOSN) is introduced, which encompasses highly sensitive antiferromagnetic nanoparticles functionalized with single‐chain fragment variable antibodies, endowing it with the capacity for discerning recognition and binding to α‐syn oligomers and triggering a switchable T1–T2 MRI signal. Significantly, ASOSN possesses the unique capability to accurately discriminate α‐syn oligomers from neuroinflammation in vivo. Moreover, ASOSN facilitates the non‐invasive and precise visualizing of endogenous α‐syn oligomers in living systems. This innovative design heralds the development of a non‐invasive visualization strategy for α‐syn oligomers, marking a pivotal advancement for early and accurate diagnosis of PD.

A Novel Multiarmed Bifunctional PEG Derivative for the Preparation of Mass Spectrometry Ion Sources with Antifouling Property and High Selectivity

“…In addition, the rich catechol groups on the surface of polydopamine allow molecules containing amino and sulfhydryl groups to be attached easily by addition reaction to form an antifouling interface . For the modification strategy, the EDC/NHS coupling strategy was usually used to immobilize the recognition molecules to PEG derivatives. , However, the reaction usually takes several hours, and the coupling is uncertain, which increases the uncontrollability. Recently, the click reaction has rapidly become a powerful tool for the immobilization of biomolecules due to its strong selectivity, high efficiency, and mild reaction condition .…”