Alpha‐synuclein immunoreactivity in minor salivary gland biopsies of Parkinson's disease patients

Cersósimo, María G.; Perandones, Claudia; Micheli, Federico; Raina, Gabriela; Beron, Ana Maria; Nasswetter, G; Radrizzani, Martı́n; Benarroch, Eduardo E.

doi:10.1002/mds.23344

Cited by 76 publications

(69 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high prevalence of dry mouth in PD patients extends our previous [31] and other [33] observations and is consistent with evidence from autopsy [6] and biopsy [10] studies demonstrating the presence of a-SYN pathology in the salivary glands.…”

Section: Discussionsupporting

confidence: 92%

“…The highest amount of a-SYN aggregates in autopsy studies of both PD and incidental Lewy body cases was found in the submandibular glands, followed, in decreased order, by the lower esophagus, stomach, small bowel, colon and rectum [4][5][6]. Recently, biopsy studies of colonic submucosa [7][8][9], minor salivary glands [10], stomach and duodenum [11], obtained from PD patients have confirmed the presence of a-SYN inclusions in these tissues.…”

Section: Introductionmentioning

confidence: 72%

See 1 more Smart Citation

Gastrointestinal manifestations in Parkinson’s disease: prevalence and occurrence before motor symptoms

et al. 2012

View full text Add to dashboard Cite

To assess the prevalence of gastrointestinal symptoms (GIS) in Parkinson's disease (PD) compared to control subjects and their timing of appearance in relationship to the onset of motor symptoms. There is a rostrocaudal gradient of alpha-synuclein (α-SYN) neuropathology in the enteric nervous system at early stages of PD with higher burden in the upper than the lower gut. However, only constipation has been recognized as a premotor gastrointestinal manifestation of PD. 129 PD patients and 120 controls underwent a structured questionnaire to assess the presence of GIS and, in PD patients, the time of their appearance respect to the onset of motor manifestations. GIS significantly more prevalent in PD patients were dry mouth, drooling, dysphagia, constipation and defecatory dysfunction. Constipation and defecatory dysfunction preceded motor manifestations. Whereas gastroparesis symptoms preceded motor manifestations, their prevalence was not significantly different from controls. Despite evidence of a higher α-SYN burden in the upper gut, only constipation and defecatory dysfunction were prominent premotor GIS of PD.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 72%

Gastrointestinal manifestations in Parkinson’s disease: prevalence and occurrence before motor symptoms

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Although it was previously reported that 2/3 subjects with PD had positive LTS in minor salivary gland biopsies, 16 we found that only 1/15 cases were positive. This case also had a positive submandibular gland biopsy.…”

contrasting

confidence: 98%

“…As others have found LTS in minor salivary glands, 15,16 we performed minor salivary gland biopsies as well on the same patients. Go to Neurology.org for full disclosures.…”

mentioning

confidence: 99%

“…12 As in previous studies, 22 the gastrointestinal (GI) tract contained LTS in most subjects with PD and there was a rostrocaudal gradient of GI LTS density, with the lower esophagus and submandibular gland having the highest densities and the colon and rectum the lowest. 12,16,23,24 Prior to performing biopsies in living patients with PD, a postmortem submandibular gland study of 92 subjects with and without PD was performed. 13 Using immunohistochemical staining for phosphorylated a-synuclein, 17,25 both large blocks Tissue volume (mm 3 ) was calculated using the formula for volume of a cylinder.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Submandibular gland needle biopsy for the diagnosis of Parkinson disease

et al. 2014

View full text Add to dashboard Cite

Objective: This study investigates salivary gland biopsies in living patients with Parkinson disease (PD). Methods:Patients with PD for $5 years underwent outpatient transcutaneous needle core biopsies (18-gauge or 16-gauge) of 1 submandibular gland. Minor salivary glands were removed via a small incision in the lower lip. Tissue was fixed in formalin and serial 6-mm paraffin sections were immunohistochemically stained for phosphorylated a-synuclein and reviewed for evidence of Lewy type a-synucleinopathy (LTS).Results: Fifteen patients with PD were biopsied: 9 female/6 male, mean age 68.7 years, mean PD duration 11.8 years. Twelve of the needle core biopsies had microscopically evident submandibular gland tissue to assess and 9/12 (75%) had LTS. Only 1/15 (6.7%) minor salivary gland biopsies were positive for LTS. Five patients had an adverse event; all were minor and transient. Conclusions:This study demonstrates the feasibility of performing needle core biopsies of the submandibular gland in living patients with PD to assess LTS. Although this was a small study, this tissue biopsy method may be important for tissue confirmation of PD in patients being considered for invasive procedures and in research studies of other PD biomarkers.

show abstract