Alpha‐pinene promotes osteoblast differentiation and attenuates TNFα‐induced inhibition of differentiation in MC3T3‐E1 pre‐osteoblasts

Min, Hyeon-Young; Son, Hyo-Eun; Jang, Won‐Gu

doi:10.1111/1440-1681.13245

Cited by 12 publications

(7 citation statements)

References 37 publications

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“…Moreover, it stimulated apoptosis in mature osteoclasts in vitro [ 26 ]. In other studies, it was shown that some terpenes (e.g., limonene, alpha-pinene) stimulated osteoblast proliferation and differentiation, which is similar to anabolic drug action used in the treatment of osteoporosis [ 22 , 27 , 28 , 29 ]. In vitro studies revealed that limonene induced osteoblastic differentiation of the murine myoblasts (C2C12) (concentration: 2.5 µM) and murine preosteoblasts (MC3T3-E1) (concentration: 0.01–1 μM) [ 27 ].…”

Section: Introductionmentioning

confidence: 92%

“…Moreover, limonene (0.01–1 μM) decreased methylglyoxal-induced endoplasmic reticulum (ER) stress and autophagy in the MC3T3-E1 cell line [ 28 ]. Another monoterpene, α-pinene (50 μM), also stimulated the differentiation and mineralization process of osteoblasts in the MC3T3-E1 cells [ 29 ]. There are also reports indicating that cinnamaldehyde prevented osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Screening Studies on Eight Commercial Essential Oils-Derived Compounds to Identify Promising Natural Agents for the Prevention of Osteoporosis

et al. 2023

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Over the years, essential oils (EOs) and their compounds have gained growing interest due to their anti-inflammatory, antimicrobial, antioxidant, and immunomodulatory properties. The aim of this study was to evaluate the effect of eight commercially available EO-derived compounds ((R)-(+)-limonene, (S)-(−)-limonene, sabinene, carvacrol, thymol, alpha-pinene (α-pinene), beta-pinene (β-pinene), and cinnamaldehyde) on the bone formation process in vitro to select the most promising natural agents that could potentially be used in the prevention or treatment of osteoporosis. Within this study, evaluation of cytotoxicity, cell proliferation, and osteogenic differentiation was performed with the use of mouse primary calvarial preosteoblasts (MC3T3-E1). Moreover, extracellular matrix (ECM) mineralization was determined using MC3T3-E1 cells and dog adipose tissue-derived mesenchymal stem cells (ADSCs). The two highest non-toxic concentrations of each of the compounds were selected and used for testing other activities. The conducted study showed that cinnamaldehyde, thymol, and (R)-(+)-limonene significantly stimulated cell proliferation. In the case of cinnamaldehyde, the doubling time (DT) for MC3T3-E1 cells was significantly shortened to approx. 27 h compared to the control cells (DT = 38 h). In turn, cinnamaldehyde, carvacrol, (R)-(+)-limonene, (S)-(−)-limonene, sabinene, and α-pinene exhibited positive effects on either the synthesis of bone ECM or/and mineral deposition in ECM of the cells. Based on the conducted research, it can be assumed that cinnamaldehyde and (R)-(+)-limonene are the most promising among all tested EO-derived compounds and can be selected for further detailed research in order to confirm their biomedical potential in the chemoprevention or treatment of osteoporosis since they not only accelerated the proliferation of preosteoblasts, but also significantly enhanced osteocalcin (OC) synthesis by preosteoblasts (the OC level was approx. 1100–1200 ng/mg compared to approx. 650 ng/mg in control cells) and ECM calcification of both preosteoblasts and mesenchymal stem cells. Importantly, cinnamaldehyde treatment led to a three-fold increase in the mineral deposition in ADSCs, whereas (R)-(+)-limonene caused a two-fold increase in the ECM mineralization of both MC3T3-E1 cells and ADSCs.

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Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

In Vitro Screening Studies on Eight Commercial Essential Oils-Derived Compounds to Identify Promising Natural Agents for the Prevention of Osteoporosis

et al. 2023

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“…It has an antithrombotic effect (Yang, Zhou et al 2011) [13], stimulates cytoprotective activity against H 2 O 2 -(Porres, Burgos, et al, 2015), (Halliwell 1992) [14,15]. It has anti-inflammatory (Falk, Hagberg, et al, 1990) [16], analgesic (Kim, Lee, et al 2015) [17], (Rufino, Ribeiro, et al 2014) [18], antioxidant properties, (Min, Son, et al, 2020) [19], as well as an expectorant and phlegm-dissolving effect, being a natural support in upper respiratory tract infections with runny nose and cough. In combination with eucalyptus oil, it allows you to perfectly unplug a blocked nose.…”

Section: What Are Essential Oils -Alpha and β-Pinenementioning

confidence: 99%

The Use of Essential Oils - Alpha and Β-Pinene in the Treatment of Covid-19

Adamski¹,

Adamska²

2021

ACMCR

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“…The α-pinene, with the chemical name of 2,6,6-trimetheylbicyclo [3.1.1] hept-2-ene, is a bicyclic monoterpene molecule found in various plants, including camphor, rosemary, and pine [6,7]. Recently, α-pinene was also been found to be presented in the highland barley Baijiu [8].…”

Section: Introductionmentioning

confidence: 99%

The Alleviated Effect of α-Pinene on Amidst Alcoholic Liver Injury Pathology Through Autophagy Activation in Mice

Luan

2023

JNFP

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Background: Alcoholic liver damage is caused by long-term and heavy alcohol consumption, which leads to many diseases and even cancer. α-Pinene has been shown to have antioxidant and anti-inflammatory activity, however, it is still unclear the relationship between α-Pinene and alcohol-induced liver injury. The potential molecular mechanisms of α-pinene in reducing alcohol-induced liver injury in mice were investigated in this study. Materials and methods: The C57BL/6 mice were randomly divided into five groups, which were the control groups (physiological saline, 0.2mL per days), alcohol group (50% alcohol, 5 mL/kg bw/day), alcohol with low/medium/high dosage α-pinene treatment group ((7.2 mg/kg bw, 14.4 mg/kg bw, 28.8 mg/kg bw, dissolved in 50% alcohol, Separately). The dosing method for mice is via oral gavage. After 8 weeks of experimentation, mouse serum and liver were collected for further testing. Result: The increased antioxidant enzyme activities demonstrated the alleviated effect of α-pinene against alcohol-induced mouse liver injury. Moreover, in liver tissues, α-pinene promoted nucleus translocation of nuclear factor-erythroid-2-related factor 2 (NRF2) and transcription of antioxidant target genes, including heme oxygenase 1 (HMOX-1 / HO-1), NAD[P]H quinone dehydrogenase 1 (NQO-1), and glutathione S-transferase alpha 1 (Gsta-1). Meanwhile, α-pinene promoted the protein expression of autophagy-related proteins and inhibited the increase of inflammatory factors caused by chronic alcohol intake. Furthermore, α-pinene partially inhibited the activation of apoptotic signaling pathways by increasing the expression of Bcl-2 and decreasing Bax and cleaved caspase-3 proteins. Conclusion: Taken together, our results indicated that α-pinene might alleviate alcoholic liver injury by reducing lipid accumulation, enhancing anti-oxidative stress and anti-inflammatory, activating autophagy, and inhibiting cell apoptosis.

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Alpha‐pinene promotes osteoblast differentiation and attenuates TNFα‐induced inhibition of differentiation in MC3T3‐E1 pre‐osteoblasts

Cited by 12 publications

References 37 publications

In Vitro Screening Studies on Eight Commercial Essential Oils-Derived Compounds to Identify Promising Natural Agents for the Prevention of Osteoporosis

In Vitro Screening Studies on Eight Commercial Essential Oils-Derived Compounds to Identify Promising Natural Agents for the Prevention of Osteoporosis

The Use of Essential Oils - Alpha and Β-Pinene in the Treatment of Covid-19

The Alleviated Effect of α-Pinene on Amidst Alcoholic Liver Injury Pathology Through Autophagy Activation in Mice

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