Alpha-mangostin dephosphorylates ERM to induce adhesion and decrease surface stiffness in KG-1 cells

Phan, Thi Kieu Trang; Ly, Thi; Tachibana, Kunihide; Kihara, Takanori

doi:10.1007/s13577-021-00651-8

Cited by 1 publication

(2 citation statements)

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“…Although microfilaments do not appear to determine the changes in cortical cytoskeletal stiffness in Drosophila melanogaster oocytes in vivo under simulated micro-and hypergravity, pre-incubation with calyculin A resulted in its increase (Figure 2E). Calyculin A stimulates the condensation of cortical actin, leading to the appearance of additional stress fibrils [22,23]. This increase in stiffness does not prevent its reduction when exposed to simulated microgravity and hypergravity compared to the corresponding control; however, the level of reduction becomes not so significant.…”

Section: Discussionmentioning

confidence: 98%

“…The oocytes were then transferred to insect saline, which, depending on the series of experiments, either did not contain any drugs or contained 2 µM cytochalasin B (for disrupting microfilaments), 10 µM colchicine (for disrupting microtubules), 40 mM acrylamide (for disrupting intermediate filaments) [21], or 100 nM calyculin A (for cortical actin condensation) [22,23]. Pre-incubation was carried out for 3 h. All manipulations with oocytes were performed at room temperature.…”

Section: Experimental Designmentioning

confidence: 99%

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The Mechanoreception in Drosophila melanogaster Oocyte under Modeling Micro- and Hypergravity

Ogneva

2023

Cells

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The hypothesis about the role of the cortical cytoskeleton as the primary mechanosensor was tested. Drosophila melanogaster oocytes were exposed to simulated microgravity (by 3D clinorotation in random directions with 4 rotations per minute—sµg group) and hypergravity at the 2 g level (by centrifugal force from one axis rotation—hg group) for 30, 90, and 210 min without and with cytochalasin B, colchicine, acrylamide, and calyculin A. Cell stiffness was measured by atomic force microscopy, protein content in the membrane and cytoplasmic fractions by Western blotting, and cellular respiration by polarography. The obtained results indicate that the stiffness of the cortical cytoskeleton of Drosophila melanogaster oocytes decreases in simulated micro- (after 90 min) and hypergravity (after 30 min), possibly due to intermediate filaments. The cell stiffness recovered after 210 min in the hg group, but intact microtubules were required for this. Already after 30 min of exposure to sµg, the cross-sectional area of oocytes decreased, which indicates deformation, and the singed protein, which organizes microfilaments into longitudinal bundles, diffused from the cortical cytoskeleton into the cytoplasm. Under hg, after 30 min, the cross-sectional area of the oocytes increased, and the proteins that organize filament networks, alpha-actinin and spectrin, diffused from the cortical cytoskeleton.

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Section: Discussionmentioning

confidence: 98%