Alpha lipoic acid treatment induces the antioxidant system and ameliorates lipid peroxidation in maize seedlings under osmotic stress

Terzi, Rabiye; Güler, Neslihan Saruhan; Guven, Funda Gul; Kadıoğlu, Asim

doi:10.2298/abs171218011t

Cited by 20 publications

(33 citation statements)

References 23 publications

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“…In this study, α-lipoic acid (1, 2-dithiolane-3-pentanoic acid; ALA) was investigated, as it is one of the most promising and effective solutions that can reduce the detrimental effects of salinity stress on sorghum plants. It can maintain its antioxidative power and protective impacts against diverse stresses in both its reduced and oxidised form [20]. Its antioxidant capacity depends on two sulfhydryl moieties [21] which enable it to scavenge free radicals and chelate metals [22].…”

Section: Introductionmentioning

confidence: 99%

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Exogenous Application of Alpha-Lipoic Acid Mitigates Salt-Induced Oxidative Damage in Sorghum Plants through Regulation Growth, Leaf Pigments, Ionic Homeostasis, Antioxidant Enzymes, and Expression of Salt Stress Responsive Genes

Youssef

Raafat

El-Yazied

et al. 2021

Plants

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In plants, α-Lipoic acid (ALA) is considered a dithiol short-chain fatty acid with several strong antioxidative properties. To date, no data are conclusive regarding its effects as an exogenous application on salt stressed sorghum plants. In this study, we investigated the effect of 20 µM ALA as a foliar application on salt-stressed sorghum plants (0, 75 and 150 mM as NaCl). Under saline conditions, the applied-ALA significantly (p ≤ 0.05) stimulated plant growth, indicated by improving both fresh and dry shoot weights. A similar trend was observed in the photosynthetic pigments, including Chl a, Chl b and carotenoids. This improvement was associated with an obvious increase in the membrane stability index (MSI). At the same time, an obvious decrease in the salt induced oxidative damages was seen when the concentration of H2O2 and malondialdehyde (MDA) was reduced in the salt stressed leaf tissues. Generally, ALA-treated plants demonstrated higher antioxidant enzyme activity than in the ALA-untreated plants. A moderate level of salinity (75 mM) induced the highest activities of superoxide dismutase (SOD), guaiacol peroxidase (G-POX), and ascorbate peroxidase (APX). Meanwhile, the highest activity of catalase (CAT) was seen with 150 mM NaCl. Interestingly, applied-ALA led to a substantial decrease in the concentration of both Na and the Na/K ratio. In contrast, K and Ca exhibited a considerable increase in this respect. The role of ALA in the regulation of K+/Na+ selectivity under saline condition was confirmed through a molecular study (RT-PCR). It was found that ALA treatment downregulated the relative gene expression of plasma membrane (SOS1) and vacuolar (NHX1) Na+/H+ antiporters. In contrast, the high-affinity potassium transporter protein (HKT1) was upregulated.

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Section: Introductionmentioning

confidence: 99%

“…Under salt stress conditions, ALA was reported to mitigate oxidative damage and enhance growth and root formation of canola seedlings [23]. Moreover, exogenous ALA has been suggested to improve photosynthesis and induce tolerance mechanisms of several plant species under diverse environmental stresses [20,24,25].…”

Section: Introductionmentioning

confidence: 99%

Exogenous Application of Alpha-Lipoic Acid Mitigates Salt-Induced Oxidative Damage in Sorghum Plants through Regulation Growth, Leaf Pigments, Ionic Homeostasis, Antioxidant Enzymes, and Expression of Salt Stress Responsive Genes

Youssef

Raafat

El-Yazied

et al. 2021

Plants

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“…Lipoic acid (LA) is a non-enzymatic low-molecular-weight antioxidant molecule that can eliminate ROS and regulate the ROS detoxifying systems in plant cells [ 15 ]. LA has special properties among all non-enzymatic antioxidants and can work both in oxidized LA and reduced dihydrolipoic acid (DHLA) forms [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Lipoic Acid Combined with Melatonin Mitigates Oxidative Stress and Promotes Root Formation and Growth in Salt-Stressed Canola Seedlings (Brassica napus L.)

et al. 2021

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Lipoic acid (LA) and melatonin (MT) are pleiotropic molecules participating in plant stress resistance by modulating cellular biochemical changes, ion homeostasis, and antioxidant enzyme activities. However, the combined role of these two molecules in counteracting the detrimental impacts of salinity stress is still unknown. In the present study, we determined the effects of exogenous LA (0.5 µM), MT (1 µM) and their combination (LA + MT) on growth performance and biomass accumulation, photosynthetic pigments, enzymatic and non-enzymatic antioxidant activities, and ions homeostatic in canola (Brassica napus L.) seedlings under salinity stress (0, 100 mM) for 40 days. The results indicate that exogenous application of LA + MT improved the phenotypic growth (by 25 to 45%), root thickness (by 68%), number of later lateral roots (by 52%), root viability (by 44%), and root length (by 50%) under salinity stress. Moreover, total soluble protein, chlorophyll pigments, the concentration of superoxide dismutase (SOD), catalase peroxidase (CAT), and ascorbic peroxidase (ASA) increased with the presence of salt concentration into the growth media and then decreased with the addition of LA + MT to saline solution. Leaf protein contents and the degradation of photosynthetic pigments were lower when LA + MT treatments were added into NaCl media. The proline and phenol contents decreased in the exogenous application of LA + MT treatments more than individual LA or MT treatments under the salinity stress. The incorporation of LA or MT or a combination of LA + MT to saline solution decreased salinity-induced malondialdehyde and electrolyte leakage. In conclusion, the alteration of metabolic pathways, redox modulation, and ions homeostasis in plant tissues by the combined LA and MT application are helpful towards the adaptation of Brassica napus L. seedlings in a saline environment. The results of this study provide, for the first time, conclusive evidence about the protective role of exogenous LA + MT in canola seedlings under salinity stress.

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“…For the water potential level ψma = -1.5Mpa, the increase was greater in BR 16 (106%), while in Embrapa 48 the increase was only 56%. Several studies illustrate the increase in lipid peroxidation under water stress: in Sweet corn (Zea mays) (Terzi, Güler, Güven, & Kadioglu, 2018); Rice (Oryza sativa) (Qureshi et al, 2018;Gorthi et al, 2019) and Sorghum (Sorghum bicolor) (Guo et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Physiological alterations and enzymatic evaluation of soybean cultivars under water deficit

Gomes

Pimenta

Amaral

et al. 2021

ASB J.

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Drought is one of the main abiotic factors limiting agricultural productivity, capable of having a major impact on the yield of most crops. The knowledge of the physiological and biochemical mechanisms that differentiate resistance and susceptibility to water deficit among soybean strains can be used in the generation of more tolerant cultivars. In this sense, the objective of this study was to characterize physiologically, two soybean cultivars with different patterns of tolerance to drought in the field, by determining photosynthetic rates, lipid peroxidation levels and antioxidant enzyme activity under three levels of water potential. Upon reaching the V4 development stage, the irrigation of the plants was suspended and three data collection were performed: full irrigation (control); moderate water deficit (Ψ = -1.5 ± 0.2 MPa) and severe deficit (Ψ = -3.0 MPa ± 0.2 MPa). Variations in perspiration rate, stomatal conductance, as well as decrease in photosynthetic rate were significant between the two cultivars, where the water potentials in cultivar BR 16 anticipated on average two days achieving the same water potentials in Embrapa 48 cultivar, thus presenting better efficiency in water use. In addition, the increased activity of enzymes and lipid peroxidation were more significant in the cultivar BR 16, demonstrating that this cultivar is less tolerant to drought than Embrapa 48 cultivar, corroborating to agronomic data previously found in the field.

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Alpha lipoic acid treatment induces the antioxidant system and ameliorates lipid peroxidation in maize seedlings under osmotic stress

Cited by 20 publications

References 23 publications

Exogenous Application of Alpha-Lipoic Acid Mitigates Salt-Induced Oxidative Damage in Sorghum Plants through Regulation Growth, Leaf Pigments, Ionic Homeostasis, Antioxidant Enzymes, and Expression of Salt Stress Responsive Genes

Exogenous Application of Alpha-Lipoic Acid Mitigates Salt-Induced Oxidative Damage in Sorghum Plants through Regulation Growth, Leaf Pigments, Ionic Homeostasis, Antioxidant Enzymes, and Expression of Salt Stress Responsive Genes

Lipoic Acid Combined with Melatonin Mitigates Oxidative Stress and Promotes Root Formation and Growth in Salt-Stressed Canola Seedlings (Brassica napus L.)

Physiological alterations and enzymatic evaluation of soybean cultivars under water deficit

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