Alpha glucosidase inhibition by stem extract of <i>Tinospora cordifolia</i>

Chougale, Ashok D.; Ghadyale, Varsha Adhikrao; Panaskar, Shrimant N.; Arvindekar, Akalpita U.

doi:10.1080/14756360802565346

Cited by 87 publications

(48 citation statements)

References 28 publications

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“…Many food materials that contain inhibitors of carbohydrate-digestive enzymes are known, [10][11][12][13] but we have particularly been interested in inhibitors of glucose transport in the small intestine. Only few materials for functional foods have been reported as inhibitors of glucose absorption, such as gymnemic acids form leaf of Gymnema sylvestre, 14) and phloridzin from bark of apple tree.…”

Section: Discussionmentioning

confidence: 99%

Peach Leaf Contains Multiflorin A as a Potent Inhibitor of Glucose Absorption in the Small Intestine in Mice

Shirosaki

Gotö²,

Hirooka³

et al. 2012

Biological & Pharmaceutical Bulletin

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Peach leaf extract has anti-hyperglycemic effects on the postprandial blood glucose level in glucoseloaded mice. In our previous study, the mechanism of action was considered to be the inhibition of glucose absorption in the small intestine. To elucidate the active principle in peach leaf, purification of the active compound and a structure determination were performed. With the use of bioassay-guided fractionation using glucose-loaded mice, the acetylated kaempferol glycoside multiflorin A (MFA), a potent inhibitor of glucose absorption from the intestine, was isolated from the MeOH extract of leaf of the edible peach Prunus persica. The structure was identified by HPLC using thiazolizine derivatives and by an analysis of its spectral data. The inhibitory effect of MFA against glucose absorption was demonstrated in the dose dependent manner in mice. However, as the deacetylated analog of MFA, multiflorin B did not show the activity at the in vivo, the activity of MFA was suggested to depend on the acetyl group on the sugar moiety. This is the first report of anti-hyperglycemic activity of MFA in peach leaf extract. MFA may be useful in functional foods or medicines for preventing the postprandial absorption of glucose in hyperglycemia.

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Section: Discussionmentioning

confidence: 99%

Peach Leaf Contains Multiflorin A as a Potent Inhibitor of Glucose Absorption in the Small Intestine in Mice

Shirosaki

Gotö²,

Hirooka³

et al. 2012

Biological & Pharmaceutical Bulletin

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“…Alpha glucosidase was isolated as reported earlier [6]. Briefly normal healthy rats fasting for 20 h were sacrificed by cervical dislocation.…”

Section: Methodsmentioning

confidence: 99%

Effective Control of Postprandial Glucose Level through Inhibition of Intestinal Alpha Glucosidase byCymbopogon martinii(Roxb.)

Ghadyale

Takalikar

Haldavnekar

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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Inhibition of intestinal alpha glucosidase plays a major role in preventing rise in postprandial glucose level in diabetics. Cymbopogon martinii (CM) (family Poaceae) is used in traditional Indian medicine in treatment of diabetes mellitus. The alpha glucosidase inhibitory action of the plant is studied. The active component was separated using hot water extraction of the whole plant powder, differential solvent extraction, and silica gel column chromatography. The 30 : 70 toluene : ethyl acetate fraction showed optimum activity. The silica gel chromatography fraction demonstrated 98, 98, and 68% inhibition for starch, maltose, and sucrose, respectively, at 5 mg/kg body weight of rats. Intestinal absorption studies using noneverted intestinal sacs, as well as in vivo studies in streptozotocin-induced diabetic rats using oral glucose tolerance with maltose and sucrose load, revealed better inhibition of alpha glucosidase as compared to acarbose. Kinetic studies using Lineweaver Burk plot showed mixed to noncompetitive type of inhibition by CM. In vivo studies with maltose load of 2 mg and 3 mg/gm body weight showed a noncompetitive pattern of inhibition at 5 mg/kg body weight of CM as against 60 mg/kg body weight of acarbose. Thus CM is more effective alpha glucosidase inhibitor and at lower concentration than acarbose.

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“…This reduction was found 27,28 Similar studies conducted on Tc suggest that the hypoglycemic activity is possibly due to inhibition of salivary and pancreatic amylase and glucosidase. 29 Due to its blood glucose lowering activity Tc is also reported to be used as an important constituent in many polyherbal formulations prepared for the treatment of diabetes. One Ayurvedic polyherbal formulation "Ilogen-Excel," which contains T. cordifolia as one of the constituents, when administered at the dose of 50 and 100 mg/kg for 60 days has shown significant decrease in blood glucose levels and increase in plasma insulin, hepatic glycogen, and total hemoglobin.…”

Section: Discussionmentioning

confidence: 99%

Effect of Tinospora cordifolia as an add - on therapy on the blood glucose levels of patients with Type 2 diabetes

Mishra

Verma

Bhattacharya

et al. 2015

Int J Basic Clin Pharmacol

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Alpha glucosidase inhibition by stem extract of Tinospora cordifolia

Cited by 87 publications

References 28 publications

Peach Leaf Contains Multiflorin A as a Potent Inhibitor of Glucose Absorption in the Small Intestine in Mice

Peach Leaf Contains Multiflorin A as a Potent Inhibitor of Glucose Absorption in the Small Intestine in Mice

Effective Control of Postprandial Glucose Level through Inhibition of Intestinal Alpha Glucosidase byCymbopogon martinii(Roxb.)

Effect of Tinospora cordifolia as an add - on therapy on the blood glucose levels of patients with Type 2 diabetes

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