Alpha Chlorohydrin: Studies on the Mechanism of Action in Male Swine

La, Johnson; Vg, Pursel

doi:10.2527/jas1973.3751207x

Cited by 12 publications

(6 citation statements)

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“…Spermatozoan motility was reduced by 4 days after the first injection of Il(-chlorohydrin and this confirms findings in other studies in the ram (BrownWoodman et al 1974;Kreider and Dutt 1970) and boar (Johnson and Pursel 1973). The decline and recovery of motility followed a similar pattern to metabolic activity and fertility .…”

Section: Discussionsupporting

confidence: 89%

“…It may be noted in this regard that the cauda epididymis of the rat concentrates IX-chlorohydrin from the blood (Crabo and Appelgren 1972;Edwards et al 1975). Our conclusion is also supported by the observations that spermatozoa recovered from the cauda epididymis of the boar are infertile within 7 h of the animals being fed IX-chlorohydrin (Johnson and Pursel 1973) and by the fact that a marked decrease in fertility occurs within 3 days of the daily injection of IX-chlorohydrin in the hamster (Lubicz-Nawrocki and Chang 1974). The normal composition of fluid in the lumen of the epididymis is dependent upon an intact epithelium which has the capacity for both resorption and secretion.…”

Section: Discussionsupporting

confidence: 80%

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Disruption of the Metabolism, Motility and Morphology of Spermatozoa by Injection of a-Chlorohydrin Into Rams

Pd¹,

White²

1976

Aust. Jnl. Of Bio. Sci.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 80%

Disruption of the Metabolism, Motility and Morphology of Spermatozoa by Injection of a-Chlorohydrin Into Rams

Pd¹,

White²

1976

Aust. Jnl. Of Bio. Sci.

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“…Boar semen contains 2-3 mM-lactate (Mann & Lutwak-Mann, 1981) and this could be sufficient to have a similar protective effect. Nevertheless, boars given -chlorohydrin at 5 mg/kg/day for^5 days were infertile (Johnson & Pursel, 1973). All the conditions which produced a decline in the ATP concentration relative to the appropri¬ ate controls also produced an accumulation of fructose 1,6-bisphosphate and of trióse phosphates.…”

Section: The Effect Ofdifferent Sugarsmentioning

confidence: 94%

The concerted effect of -chlorohydrin and glucose on the ATP concentration in spermatozoa is associated with the accumulation of glycolytic intermediates

Ford¹,

Harrison²

1986

Reproduction

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Summary. In the absence of a glycolysable sugar the effect of 1 mM-RS-\g=a\-chlorohydrin on the ATP concentration in ram or boar spermatozoa was relatively small but the addition of 0\m=.\10or 0\m=.\03 mM-glucose initiated a rapid loss of ATP. When the spermatoza were incubated with 0\m=.\05mM-RS-\g=a\-chlorohydrin, the addition of 1\m=.\0mM (ram) or 0\m=.\06mM (boar)-glucose was required to produce ATP dissipation. In ram spermatozoa treated with 0\m=.\05or 1\m=.\00mM-RS-\g=a\-chlorohydrin, ATP loss was caused by 10 mM\x=req-\ fructose or 10mM-mannose but not by 10mM-glycerol or 10mM-inositol. In boar spermatozoa incubated with 1 mM-RS-\g=a\-chlorohydrin the addition of 10mM-L-lactate plus 1\ m=. \ 0 mM-pyruvate protected the spermatozoa against the ability of 1\m=.\0 mM-glucose to produce a decline in ATP concentration.Every combination of treatments capable of inducing a marked decline in ATP concentration also caused a dramatic (20\p=n-\100-fold) increase in the concentration of fructose 1,6-bisphosphate. An increase in fructose 1,6-bisphosphate concentration was never observed when the ATP concentration was unaffected. We conclude that it is very probable that the concerted effect of a-chlorohydrin and glycolysable sugar is responsible for the contraceptive action of \g=a\-chlorohydrin in vivo and that fructose 1,6-bisphosphate is implicated in its mechanism of action.

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“…Since -chlorohydrin is an alkyl halide, it may function as an alkylating agent, as indicated by its reactivity with cysteine in vitro and the appearance in urine of alkylated cysteine metabolites after administration in vivo (Jones, Davis, Edwards & Jackson, 1969). Johnson & Pursel (1973) found that the cauda epididymidis in the boar was the site of a-chloro¬ hydrin activity and suggested that -chlorohydrin might block an enzyme(s) on the sperm cell. These sites may be those of the glycosidases and sulphatases which are associated with spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

“…Vickery, Erickson & Bennett (1974) found that the motility and transport of spermatozoa recovered after mating was inhibited as a result of changes in semen composition. Johnson & Pursel (1973) proposed that the mechanism of action of -chlorohydrin might involve blockage of an enzyme site or sites on the sperm cell.…”

Section: Introductionmentioning

confidence: 99%