Alpha-Arbutin Promotes Wound Healing by Lowering ROS and Upregulating Insulin/IGF-1 Pathway in Human Dermal Fibroblast

Polouliakh, Natalia; Ludwig, Vanessa; Meguro, Akira; Kawagoe, Tatsukata; Heeb, Oliver; Mizuki, Nobuhisa

doi:10.3389/fphys.2020.586843

Cited by 17 publications

(12 citation statements)

References 24 publications

(45 reference statements)

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“…Polouliakh et al indicated that α-ARB promoted wound healing by activating the insulin-like growth factor 1 receptor (IFG1R) pathway in human dermal broblasts [32], and alterations induced by ARB treatment should be explored. On the other hand, Nrf2-mediated redox regulation is not restricted to HO-1.…”

Section: Discussionmentioning

confidence: 99%

Arbutin-Modified Microspheres Prevent Osteoarthritis Progression by Mobilizing Local Anti-Inflammatory and Antioxidant Responses

Jin

Liu

Jiang

et al. 2022

Preprint

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Osteoarthritis (OA), a common chronic degenerative disease, is considered the leading cause of disability worldwide. However, the current clinical treatments for OA are still unsatisfactory. Arbutin (ARB) is a major active ingredient of the Chinese medicinal herb cowberry leaf, which exerts potential antioxidant and anti-inflammatory activity. Based on a microfluid platform, we fabricated ARB-loaded gelatine methacryloyl-Liposome (GM-Lipo@ARB) microspheres that showed long-term release and excellent cartilage-targeting effects. The ARB-loaded microspheres effectively reduced the inflammatory response in interleukin (IL)-1β-treated arthritic chondrocytes. Moreover, GM-Lipo@ARB microspheres regulated cartilage extracellular matrix (ECM) homeostasis by upregulating nuclear factor erythroid 2-related factor 2 (Nrf2)-associated antioxidant properties. Intraarticular injection of GM-Lipo@ARB microspheres alleviated cartilage erosion in the destabilization of the medial meniscus (DMM)-mediated mouse OA model. Our results indicated that ARB-modified microspheres prevented cartilage degeneration by not only inhibiting the inflammatory reaction but also mitigating oxidative stress. This study proposes a novel ARB-laden functional microsphere for treating OA through the mobilization of local anti-inflammatory and antioxidant responses.

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Section: Discussionmentioning

confidence: 99%

Arbutin-Modified Microspheres Prevent Osteoarthritis Progression by Mobilizing Local Anti-Inflammatory and Antioxidant Responses

Jin

Liu

Jiang

et al. 2022

Preprint

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“…Combined with the KEGG results of acute wound phase, we speculated that targeting TYRP1 or DCT to inhibit the tyrosine metabolic pathway in keratinocyte will become a potential therapeutic strategy for alleviating or treating acute wound. Moreover, this speculate has been implemented in the recent studies [ 26 , 27 ], but there is no clinical research.…”

Section: Discussionmentioning

confidence: 99%

Identification of potential hub genes associated with skin wound healing based on time course bioinformatic analyses

2021

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Background The skin is the largest organ of the body and has multiple functions. Wounds remain a significant healthcare problem due to the large number of traumatic and pathophysiological conditions patients suffer. Methods Gene expression profiles of 37 biopsies collected from patients undergoing split-thickness skin grafts at five different time points were downloaded from two datasets (GSE28914 and GSE50425) in the Gene Expression Omnibus (GEO) database. Principal component analysis (PCA) was applied to classify samples into different phases. Subsequently, differentially expressed genes (DEGs) analysis, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway functional enrichment analyses were performed, and protein–protein interaction (PPI) networks created for each phase. Furthermore, based on the results of the PPI, hub genes in each phase were identified by molecular complex detection combined with the ClueGO algorithm. Results Using principal component analysis, the collected samples were divided into four phases, namely intact phase, acute wound phase, inflammatory and proliferation phase, and remodeling phase. Intact samples were used as control group. In the acute wound phase, a total of 1 upregulated and 100 downregulated DEGs were identified. Tyrosinase (TYR), tyrosinase Related Protein 1 (TYRP1) and dopachrome tautomerase (DCT) were considered as hub genes and enriched in tyrosine metabolism which dominate the process of melanogenesis. In the inflammatory and proliferation phase, a total of 85 upregulated and 164 downregulated DEGs were identified. CHEK1, CCNB1 and CDK1 were considered as hub genes and enriched in cell cycle and P53 signaling pathway. In the remodeling phase, a total of 121 upregulated and 49 downregulated DEGs were identified. COL4A1, COL4A2, and COL6A1 were considered as hub genes and enriched in protein digestion and absorption, and ECM-receptor interaction. Conclusion This comprehensive bioinformatic re-analysis of GEO data provides new insights into the molecular pathogenesis of wound healing and the potential identification of therapeutic targets for the treatment of wounds.

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“…Polouliakh et al conducted in silico comparative genomics analysis in human dermal fibroblasts treated with α-arbutin, and identified transcription factors with a potential role in tumor suppression, toxicity response, and wound healing [148]. α-arbutin upregulated Nrf2 transcription factor which consequently activates target genes involved in antioxidant defense.…”

Section: Antioxidant Properties Of Arbutin and α-Arbutinmentioning

confidence: 99%

The Potential of Dietary Antioxidants

2022

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and a specialist in "Food Science", with a Ph.D. in "Pharmacologically Active Natural Compounds". She teaches "Food chemistry", "Spectroscopic methods in food analysis", and "Science of dietary supplements of plant origin" as a Research Professor at the Federico II University. The scientific activity of Prof. Dini focuses on the study of natural substances and has mainly developed around four themes: a) Chemical study of primary and secondary metabolites of interest for food; b) Nutraceutical and nutricosmeceutical studies of phytochemicals from food plants; c) Development of analytical methods for the dosage of metabolites of food and nutricosmeceutical interest; d) Study of environmentally friendly agronomic techniques capable of improving the nutraceutical interest of food products.

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Alpha-Arbutin Promotes Wound Healing by Lowering ROS and Upregulating Insulin/IGF-1 Pathway in Human Dermal Fibroblast

Cited by 17 publications

References 24 publications

Arbutin-Modified Microspheres Prevent Osteoarthritis Progression by Mobilizing Local Anti-Inflammatory and Antioxidant Responses

Arbutin-Modified Microspheres Prevent Osteoarthritis Progression by Mobilizing Local Anti-Inflammatory and Antioxidant Responses

Identification of potential hub genes associated with skin wound healing based on time course bioinformatic analyses

The Potential of Dietary Antioxidants

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