Alpha-Antitrypsin in Uterine Secretions

Quantitative Determination of High Molecular Weight Serum Proteinase Inhibitors in Human Semen

“…in the seminal plasma of andrological patients are in good agreement with the data reported by Schumacher (1969) indicating only insignificant differences in ejaculates according to the semen quality characterized by "good", "fair" and "poor".…”

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Human seminal plasma contains high molecular weight and low molecular weight proteinase inhibitors. HUSI-I1 is a potent inhibitor of the sperm specific proteinase acrosin (Schiessler et al -1975) involved in the penetration of the zona pellucida by the spermatozoa (Zaneveld et al -1972).Since there are only few data available on high molecular weight serum proteinase inhibitors in human semen (Herrmann and Hermann -1969;Schumacher -1969) quantitative determinations of serum proteinase inhibitors were performed in seminal plasma of andrological patients using the radial immunodiffusion technique.

Material and Methodsservice of the Department of Dermatology was collected immediately after masturbation (4-6 days of sexual abstinence). The following serum proteinase inhibitors are wellknown: alpha1 mtitrypsin, alpha1 ,x-antichymotrypsin, inter-alpha-trypsin inhibitor, antithrombin 111, alpha2-neuramino-glycoprotein and alpha2-macroglobulin (Heimburger et al -1971).

In contrast low molecular weight proteinase inhibitors are acidstable and are specific products of the male genital tract secretions (Fink et al -1971;Zaneveld et al -1971).

The latter group consists of a trypsin-chymotrypsin inhibitor (HUSH, MW 11,000) and a trypsin-acrosin inhibitor MW 6,200).

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“…The latter group consists of a trypsin-chymotrypsin inhibitor (HUSH, MW 11,000) and a trypsin-acrosin inhibitor (HUSI-11, MW 6,200). HUSI-I1 is a potent inhibitor of the sperm specific proteinase acrosin (Schiessler et al -1975) involved in the penetration of the zona pellucida by the spermatozoa (Zaneveld et al -1972).Since there are only few data available on high molecular weight serum proteinase inhibitors in human semen (Herrmann and Hermann -1969;Schumacher -1969) quantitative determinations of serum proteinase inhibitors were performed in seminal plasma of andrological patients using the radial immunodiffusion technique.…”

Effects of season and dietary ascorbic acid on some biochemical characteristics of rainbow trout (Oncorhynchus mykiss) semen

“…This activity is also present in mammalian semen as low and highmolecular weight molecules (Schumacher 1970;Mann and Lutwak-Mann 1981;Torska 1989;Espafia et al 1991;Laurell et al 1992;Zheng et al 1994). Fish APAs have a high molecular weight, as it was eluted at the region of about 90 kDa after gel filtration on Sephacryl S-200 HR .…”

“…A highmolecular-weight seminal plasma protein(s) associated with the activity suggests its similarity to blood proteinase inhibitors belonging to a large group of proteins known as serpins (serine proteinase inhibitors, Patterson et al 1991). They were also identified in human seminal plasma (Schumacher 1970;Espafia et al 1991;Laurell et al 1992;Zheng et al 1994). The serpins are involved in regulation of many proteolytic events, such as the coagulation pathway, fibrinolysis, complement activation, and inflammation.…”