Background and objectives: The introduction of the semi-dwarfing Reduced Height (Rht) genes in wheat led to dramatic yield increases. The two most common forms of the gene, Rht-B1b and Rht-D1b have been widely incorporated into wheat varieties. However, despite their agronomic benefits and prevalence in modern wheat cultivars, much less is known regarding their impact on end-use quality and nutrition. For this study, we compared near-isogenic lines (NILs) carrying either Rht-B1b, Rht-D1b, Rht-8 or no semi-dwarfing alleles. Findings: Our agronomic results agreed with previous studies; we observed a 25% height reduction, 13% yield increase, and a 2% decrease in grain protein content in the semi-dwarf NILs. However, despite the decreased protein content, the Rht-B1b/Rht-D1b NILs had increased bake mixing time (33%), but reduced loaf volume (7%). We also observed that although the semi-dwarfing alleles decreased kernel weight by 15%, they were associated with a 2% increase in flour yield. Flours prepared from the semi-dwarf NILs had decreased Zinc, Iron, and Manganese while having increased levels of Potassium and Calcium. Conclusions: These findings demonstrate that while Rht-B1b/Rht-D1b negatively impact kernel size and protein content they positively impact flour yield and dough mixing strength. Significance and novelty: The most common wheat semi-dwarf alleles decrease kernel size, protein, and micronutrient content while increasing flour yield.
K E Y W O R D Sbread making, end-use quality, nutrition, Rht, wheat