Alpha 5, alpha 3, and non-alpha 3. Three clustered avian genes encoding neuronal nicotinic acetylcholine receptor-related subunits.

The neuronal α6 subunit forms functional heteromeric acetylcholine receptors in human transfected cells

“…The α 6 gene was isolated as a set of overlapping recombinants from a home-made chicken erythrocyte DNA library in the vector λL47. Preparation of mRNA, cloning and screening procedures were conducted as described in detail by Couturier et al (1990). The α 6 cDNA has been allotted the EMBL accession number X83889.…”

“…The cDNAs encoding chick α 3 , β 2 and β 4 were cloned into the Flip vector, where transcription is under the control of the SV40 early promoter (Couturier et al, 1990). To maximize expression of the α 6 subunit, its cDNA was subcloned in the pMT3 expression vector (Swick et al, 1992), under the control of the efficient adenovirus major late promoter.…”

“…Upon heterologous expression, the α 2 , α 3 and α 4 subunits form functional heteromeric receptors with either β 2 or β 4 subunits, whereas α 7 , α 8 and α 9 give rise to functional homomeric receptors. These various species have different drug sensitivities and physiological properties, including distinct single channel profiles (Papke et al, 1989;Couturier et al, 1990;Papke & Heinemann, 1991;Hussy et al, 1994;Gopalakrishnan et al, 1995;Ragozzino et al, 1997). By contrast, the α 5 , α 6 and β 3 subunits have long been considered to be orphan subunits as heterologous expression, either singly or with any of the available subunits, has failed to yield functional receptors.…”

Agonist Selectivity and Ion Permeation in the α3β4 Ganglionic Nicotinic Receptor

“…Neurons in the autonomic ganglia that innervate downstream targets receive cholinergic input from the CNS (Wehrwein et al, 2016). The predominant neurotransmitter receptor in these neurons is the a3b4 nicotinic acetylcholine receptor, commonly referred to as the ganglionic nicotinic receptor (Conroy and Berg, 1995;Couturier et al, 1990;Skok, 2002).…”

Cell-specific expression of theα9 n-ACh receptor subunit in auditory hair cells revealed by single-cell RT-PCR

“…1989a, Duvoisin 1989Couturier et al 1990 a, b;Schoepfer et al 1990). Recently Housley and coworkers reported the ex pression of two subunits, 0(5 (Couturier et 1990£) and(34 (Duvoisin et al 1989), of neuronal nicotinic acetylcholine receptors (n-AChRs) in rat cochlea, which were detected by the reverse transcription polymerase chain reaction (r t -pc r ) using total cochlea cDNA (Housley et al 1994). As the a5 subunit has not yet been functionally expressed, the pharmacological profile and the C a2+ permeability of receptor channels formed by this subunit combination is not known.…”