Aloperine attenuates hydrogen peroxide-induced injury via anti-apoptotic activity and suppression of the nuclear factor-κB signaling pathway

Ren, Dongliang; Ma, Weisong; Guo, Bao-Zhen; Wang, Shunyi

doi:10.3892/etm.2016.3962

Cited by 21 publications

(11 citation statements)

References 20 publications

(23 reference statements)

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“…The results showed that the major indicators of oxidative stress, including ROS, MDA, GST, were increased, while GPX was decreased in RSC96 cells after HG treatment, but this was reversed by aloperine. Thus, in Schwan cells aloperine alleviated oxidative stress induced by HG, which was similar to previous studies [23,25]. Aloperine attenuated alterations in oxidative stress biomarkers induced by monocrotaline in pulmonary hypertensive rats [23].…”

Section: Discussionsupporting

confidence: 89%

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Aloperine attenuates high glucose-induced oxidative injury in Schwann cells via activation of NRF2/HO-1 pathway

Chen¹,

Ma²,

Wang³

et al. 2020

Trop. J. Pharm Res

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Purpose: To determine the involvement of nuclear factor erythroid 2-related factor 2 (NRF2) and heme oxygenase-1 (HO-1) in the action of aloperine on Schwann cell injury caused by high glucose (HG).Methods: Cell viability was determined using MTT assay while the release of lactate dehydrogenase (LDH) was determined by biochemical assay. Apoptosis was assessed using flow cytometry, while the levels of malondialdehyde (MDA) were determined by Annexin V-FIT staining. Glutathione Stransferase (GST), glutathione peroxidase (GPX), and reactive oxygen species (ROS) were determined using enzyme-linked immunosorbent assay.Results: Treatment with HG suppressed RSC96 cell viability and increased LDH release, while aloperine reversed these results (p < 0.05). Apoptosis of RSC96 cells was induced by HG stimulation, but was abolished by aloperine. The levels of ROS, MDA, and GST were enhanced in cells followingtreatment with HG, but was reversed by aloperine (p < 0.05). The decreased level of GPX caused by HG in RSC96 cells was elevated by aloperine. Moreover, aloperine upregulated NRF2 and HO-1 in RSC96 cells treated with HG (p < 0.05).Conclusion: Aloperine attenuates HG-induced oxidative injury in Schwann cells via activation of NRF2/HO-1 pathway, suggesting its potential as a potent drug for the management of diabetic peripheral neuropathy. Keywords: Aloperine, Schwann cells, High glucose, Oxidative stress, NRF2, HO-1

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Section: Discussionsupporting

confidence: 89%

“…Aloperine attenuated alterations in oxidative stress biomarkers induced by monocrotaline in pulmonary hypertensive rats [23]. Aloperine also reduced oxidation activity induced by H2O2 [25]. Therefore, these results indicated that aloperine alleviated HG-induced oxidative stress in RSC96 cells.…”

Section: Discussionmentioning

confidence: 70%

Aloperine attenuates high glucose-induced oxidative injury in Schwann cells via activation of NRF2/HO-1 pathway

Chen¹,

Ma²,

Wang³

et al. 2020

Trop. J. Pharm Res

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“…Aloperine is a quinolizidine alkaloid that has been isolated from S. alopecuroides extract [ 3 ]. It has been reported to possess multiple pharmacological activities, including antimicrobial, antiviral, anti-allergic, anti-renal injury, anti-neuronal injury, anti-inflammatory, antinociceptive, and antitumor effects [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ]. The antitumor effects of aloperine have also been demonstrated on several human cancer cells, including colon cancer, lung cancer, and multiple myeloma [ 2 , 8 , 13 ]; however, there is a lack of studies on its antitumor effects on human thyroid cancers and the associated mechanisms.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Antitumor Activity of Aloperine on Human Thyroid Cancer Cells through Caspase-Dependent Apoptosis

Lee

Chen

Lin

et al. 2018

IJMS

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The global incidence of thyroid cancer, one of the most common endocrine malignancies, is especially high among women. Although most patients with thyroid cancers exhibit a good prognosis with standard treatment, there are no effective therapies for patients with anaplastic thyroid cancers or cancers that have reached an advanced or recurrent level. Therefore, it is important to develop highly effective compounds for treating such patients. Aloperine, a natural compound isolated from Sophora alopecuroides, has been reported to possess antioxidant, anti-inflammatory, anti-neuronal injury, anti-renal injury, antitumor, anti-allergic, and antiviral properties. In this study, we show that aloperine can inhibit cell growth in human anaplastic thyroid cancers and multidrug-resistant papillary thyroid cancers. Moreover, it could suppress in vitro tumorigenesis and promote cellular apoptosis. Further analysis demonstrated the involvement of caspase-dependent apoptosis, including intrinsic and/or extrinsic pathways, in aloperine-induced cellular apoptosis. However, cell cycle regulation was not detected with aloperine treatment. This study suggests the potential therapeutic use of aloperine in human anaplastic thyroid cancers and multidrug-resistant papillary thyroid cancers.

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“…It exhibits various pharmacological activities, including antibacterial, anti-allergic, antiviral, anti-renal injury, anti-nerve injury, anti-inflammatory, and anti-tumor effects. [14][15][16][17][18] The antitumor effects of ALO have been demonstrated in several human cancer cells, including lung, colon, and multiple myeloma. [18][19][20][21] However, few studies explored its anti-tumor effect on BCs and its related mechanism.…”

Section: Introductionmentioning

confidence: 99%

<p>Aloperine Exerts Antitumor Effects on Bladder Cancer in vitro</p>

Zhang¹,

Liang²,

Liu³

et al. 2020

OTT

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Background: Human bladder cancer is the most common malignant tumor of the urinary system and one of the 10 most common tumors of the whole body. Although most patients with bladder cancer exhibit a good prognosis with standard treatment, effective therapies for patients with a recurrent or advanced bladder cancer are unavailable. Therefore, highly effective drugs to treat such patients need to be developed. Aloperine (ALO), a natural compound isolated from Sophora alopecuroides, has antitumor properties. However, the role of ALO in human bladder cancer remains unclear. Methods: In the present study, MTT was used to detect the cytotoxic effect of ALO on human BC cell line EJ and human urothelium cell line SV-HUC-1cells. Meanwhile, in order to investigate the effects of ALO on the proliferation, apoptosis, migration, and invasion of BC EJ cells and its mechanism by Cell Counting Kit-8 (CCK-8) assay, immunofluorescence, Hoechst 33342 staining, wound scratch assay, transwell migration and invasion assay, Western blot analysis. Results: ALO can inhibit the proliferation and invasion of human bladder cancer EJ cells, and is low-toxic to human urothelium cells. Moreover, it can promote cellular apoptosis in vitro. Further analysis demonstrated the involvement of Caspase-dependent apoptosis following ALO treatment. ALO also downregulated the protein expression levels of Ras, p-Raf1 and p-Erk1/2. Conclusion: ALO is a potential drug for human bladder cancer therapy.

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Aloperine attenuates hydrogen peroxide-induced injury via anti-apoptotic activity and suppression of the nuclear factor-κB signaling pathway

Cited by 21 publications

References 20 publications

Aloperine attenuates high glucose-induced oxidative injury in Schwann cells via activation of NRF2/HO-1 pathway

Aloperine attenuates high glucose-induced oxidative injury in Schwann cells via activation of NRF2/HO-1 pathway

In Vitro Antitumor Activity of Aloperine on Human Thyroid Cancer Cells through Caspase-Dependent Apoptosis

<p>Aloperine Exerts Antitumor Effects on Bladder Cancer in vitro</p>

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