Aloe barbadensis Miller, usually referred to Aloe vera, has existed around since the dawn of time. It is a North African plant that has a variety of medicinal properties. This work was a contribution to studying the physico-chemical characteristics, the photochemical content, and the biological properties of Aloe Barbadensis Miller, a plant collected from a local garden located in the small town of Berriane (region of Ghardaïa, Algeria). The antioxidant activity was determined utilizing the DPPH method, and the solid medium diffusion method was employed to assess the antimicrobial potential against human pathogenic bacteria a gram positive (S. aureus) bacterial strain, two gram negative bacteria (E. coli and P. aeruginosa), and one yeast (Candida albicans) and fungi. The phytochemical analysis demonstrated the existence of different phytochemical substances such as phenol, flavonoid, tannin, alkaloid, saponin, and terpenoid in aloe vera gel extract. Furthermore, the antibacterial test of Aloe vera leaf extracts revealed that the gram negative bacteria E. coli sensibility compared with the two other bacteria (S. aureus and P. aeruginosa), which showed resistance, and that this extract showed antifungal efficiency against the fungi. In addition, the aloe vera extract was given an antioxidant effect according to the DPPH free radical method. The findings of this study allowed us to confirm that Aloe vera's biological activity is principally due to the existence of various phytochemical compounds with biological activities, like phenolic compounds. According to these obtained results, A. barbadensis gel extract can be utilized to treat certain diseases by providing a natural biological active component.