Aloe-Emodin Induces Apoptosis in T24 Human Bladder Cancer Cells Through the p53 Dependent Apoptotic Pathway

Abstract

AE induced apoptosis in T24 cells is mediated through the activation of p53, p21, Fas/APO-1, Bax and caspase-3.

Emodin-Provoked Oxidative Stress Induces Apoptosis in Human Colon Cancer HCT116 Cells through a p53-Mitochondrial Apoptotic Pathway

“…In anticancer researchs, overexpression of p53 is invovled in apoptosis inducted by many natrual anticancer agents (Lin et al, 2006;Hassan et al, 2013;Rengarajan et al, 2014). It has been well demonstrated that p53 could mediate transcriptional activation of Bax and trigger mitochondrial apoptosis (Toshiyuki et al, 1995;Chipuk et al, 2004).…”

Curcumin blocks migration and invasion of mouse-rat hybrid retina ganglion cells (N18) through the inhibition of MMP-2, -9, FAK, Rho A and Rock-1 gene expression

“…The isolated cells from each treatment were lysed individually and the total protein levels were quantified. To determine the proteins levels associated with invasion and migration (Ras, GRB2, MEKK3, FAK, MKK7, JNK, ERK, MMP-9, MMP-2, Rho A, PI3K, PKC, VEGF and c-jun-p) in N18 cells, all samples were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) as described previously (11,26,27).…”

“…N18 cells (1x10 6 /wells) in DMEM medium were plated onto 6-well plates and then were treated with 15 μM curcumin for 24 and 48 h. Cells were isolated from each treatment and the total RNA was extracted using the Qiagen RNeasy mini kit as described previously (27)(28)(29). All RNA samples were reversetranscribed individually for 30 min at 42˚C with high capacity cDNA Reverse transcription kit according to the standard protocol of the supplier (Applied Biosystems).…”

“…The quantitative PCR from each sample was performed by the condition: 2 min at 50˚C, 10 min at 95˚C, and 40 cycles of 15 sec at 95˚C, 1 min at 60˚C using 1 μl of the cDNA reverse-transcribed as described above, 2X SYBR Green PCR master mix (Applied Biosystems) and 200 nM of forward and reverse primers as shown in Table I. Each assay was run on an Applied Biosystems 7300 Real-Time PCR system in triplicates and expression fold-changes were derived using the comparative C T method (27,29,30).…”

Design and evaluation of new chemotherapeutics of aloe-emodin (AE) against the deadly cancer disease: an in silico study

“…The inhibitory effect of AE on the activity and gene expression of N-acetyl transferase, which plays an initial role in the metabolism of aryl amine carcinogens, was found in human malignant melanoma cells [28]. Recently, Lin et al found that AE-induced apoptosis in T24 human bladder cancer cells was mediated through the activation of p53, p21, Fas/Apo-1, Bax, and caspase 3 [27]. Even if it was found through the work of Lee et al and Pecere et al that AE induces apoptosis in many cancer cells by increasing the concentration of Bax and by decreasing the concentrations of Bcl-2 [25,43].…”