Aloe-emodin from rhubarb (Rheum rhabarbarum) inhibits lipopolysaccharide-induced inflammatory responses in RAW264.7 macrophages

Hu, Boyang; Zhang, Hai; Meng, Xianli; Wang, Fei; Wang, Ping

doi:10.1016/j.jep.2014.03.059

Cited by 163 publications

(106 citation statements)

References 39 publications

(37 reference statements)

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“…RAW 264.7 cells were plated at 4 Â 10 4 cells/ml in 96-well plates incubated at 37°C in 5% CO 2 overnight (Hu et al, 2014). Compounds were added into each well and treated for 24 h. Alamar-Blue reagent was added into each well, and fluorescence intensity measured (excitation, 544 nm; emission, 590 nm) using a Varioskan Flash Multimode Reader (Thermo Scientific).…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

Coptisine from Coptis chinensis inhibits production of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 murine macrophage cells

Hai

et al. 2016

European Journal of Pharmacology

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Section: Cell Proliferation Assaymentioning

confidence: 99%

Coptisine from Coptis chinensis inhibits production of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 murine macrophage cells

Hai

et al. 2016

European Journal of Pharmacology

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“…In addition, another study also found that emodin treatment can ameliorate hypercholesterolemia by binding with sodium deoxycholate [11]. Until now, various pharmacological effects of AE including anti-neoplasticity [12], anti-inflammation [13], and strengthening of immunity [14], have been elucidated. However, the potential role of AE in the cardiovascular system is limited.…”

Section: Introductionmentioning

confidence: 97%

Aloe-Emodin Relieves High-Fat Diet Induced QT Prolongation via MiR-1 Inhibition and IK1 Up-Regulation in Rats

Sun

Zhao

et al. 2017

Cell Physiol Biochem

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Background/Aims: High-fat diet (HFD) causes cardiac electrical remodeling and increases the risk of ventricular arrhythmias. Aloe-emodin (AE) is an anthraquinone component isolated from rhubarb and has a similar chemical structure with emodin. The protective effect of emodin against cardiac diseases has been reported in the literature. However, the cardioprotective property of AE is still unknown. The present study investigated the effect of AE on HFD-induced QT prolongation in rats. Methods: Adult male Wistar rats were randomly divided into three groups: control, HFD, and AE-treatment groups. Normal diet was given to rats in the control group, high-fat diet was given to rats in HFD and AE-treatment groups for a total of 10 weeks. First, HFD rats and AE-treatment rats were fed with high-fat diet for 4 weeks to establish the HFD model. Serum total cholesterol and triglyceride levels were measured to validate the HFD model. Afterward, AE-treatment rats were intragastrically administered with 100 mg/kg AE each day for 6 weeks. Electrocardiogram monitoring and whole-cell patch-clamp technique were applied to examine cardiac electrical activity, action potential and inward rectifier K + current (I K1 ), respectively. Neonatal rat ventricular myocytes (NRVMs) were subjected to cholesterol and/or AE. Protein expression of Kir2.1 was detected by Western blot and miR-1 level was examined by real-time PCR in vivo and in vitro, respectively. Results: In vivo, AE significantly shortened the QT interval, action potential duration at 90% repolarization (APD 90 ) and resting membrane potential (RMP), which were markedly elongated by HFD. AE increased I K1 current and Kir2.1 protein expression which were reduced in HFD rats. Furthermore, AE significantly inhibited pro-arrhythmic miR-1 in the hearts of HFD rats. In vitro, AE decreased miR-1 expression levels resulting in an increase of Kir2.1 protein levels in cholesterol-enriched NRVMs. Conclusions: AE prevents HFD-induced QT prolongation by repressing miR-1 and upregulating its target Kir2.1. These findings suggest a novel pharmacological role of AE in HFD-induced cardiac electrical remodeling.

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“…Recent accumulating evidence shows that several species of the Rheum genus such as Rheum emodi, Rheum undulatum L., Rhizoma Rhei, Rheum ribes, Rheum palmatum L., and Rheum rhaponticum have antiallergic, antibacterial, antioxidant, anticancer, antiangiogenesis, and anti-in ammation properties [11][12][13][14][15][16][17][18][19][20]. In relation to antioxidant capacity, anthraquinone derivates are considered to be one of the major biologically active constituents of aloeemodin, rhein, emodin, chrysophanol physcion, and danthron from the rhizomes of Rheum undulatum L. and Rheum palmatum L. [11,12,19,21]. However, antioxidant capacity of Korean rhubarb, Rheum officinale Baillon, has never been investigated in spite of the rapid growing interest in using natural antioxidants and functional ingredients in foods and dietary supplements.…”

Section: Introductionmentioning

confidence: 99%

Lipid Peroxidation and Antioxidant Activities of the Aqueous Rhizome Extract of Rheum officinale Baillon

Chang

Kim

2018

Journal of Food Quality

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Generation of reactive oxygen species (ROS) is associated with dysregulation of antioxidant defense mechanisms and incidence of human diseases. e speci c aim of this study was to investigate the lipid oxidation and antioxidant activity of aqueous extract of Rheum o cinale Baillon rhizome in order to evaluate its potential as a future novel natural antioxidant resource and a functional ingredient in food and pharmaceutical formations. Total phenolic and avonoid contents of Rheum rhizome extract were dose dependently increased. Consistent with this, radical scavenging activities of Rheum rhizome extract as determined by 2,2-diphenyl-1-picrylhydrazyl assay and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity were signi cantly elevated as the concentration increased. In addition, the treatment of aqueous Rheum rhizome extract signi cantly increased ferric reducing and copper chelating activities. According to results of thiobarbituric acid reactive substance analysis, Rheum rhizome extract signi cantly delayed lipid oxidation. Preincubation with Rheum rhizome extract signi cantly inhibited tert-butyl hydroperoxide-(t-BHP-) induced ROS generation. Moreover, superoxide anion production was signi cantly lower in Rheum rhizome extract-treated RAW264.7 macrophage cells than t-BHP-incubated cells (p < 0.05). ese ndings suggest that Rheum o cinale Baillon rhizome extract has a potential as an excellent natural antioxidant agent.

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Aloe-emodin from rhubarb (Rheum rhabarbarum) inhibits lipopolysaccharide-induced inflammatory responses in RAW264.7 macrophages

Cited by 163 publications

References 39 publications

Coptisine from Coptis chinensis inhibits production of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 murine macrophage cells

Coptisine from Coptis chinensis inhibits production of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 murine macrophage cells

Aloe-Emodin Relieves High-Fat Diet Induced QT Prolongation via MiR-1 Inhibition and IK1 Up-Regulation in Rats

Lipid Peroxidation and Antioxidant Activities of the Aqueous Rhizome Extract of Rheum officinale Baillon

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