Aloe-Emodin Effects on ArylamineN-Acetyltransferase Activity in the BacteriumHelicobacter pylori

Abstract

Arylamine N-acetyltransferase (NAT) activities with p-aminobenzoic acid (PABA) and 2-aminofluorene (AF) were determined in H. pylori collected from peptic ulcer patients. Cytosols or suspensions of H. pylori with or without different concentrations of aloe-emodin co-treatment showed different percentages of AF and PABA acetylation. The data indicate that there was decreased NAT activity associated with increased aloe-emodin in H. pylori cytosols. Inhibition of growth study from H. pylori demonstrated that aloe-emodin elicited dose-dependent growth inhibition in H. pylori cultures. The report is the first finding of aloe-emodin inhibition of arylamine NAT activity in a strain of H. pylori.

Molecular mechanism of emodin action: Transition from laxative ingredient to an antitumor agent

“…13 The crude drug is also reported to be useful for the treatments of hemorrhage of the digestive system and extermination of Helicobacter pylori. [14][15][16] The dried roots of Rumex patientia L (contains emodin) have been used as purgative, depurative, and tonic in Turkish traditional medicine. 17 Even though there are a variety of compounds present in these crude extracts, emodin happens to be a principal component responsible for the biological properties associated with it.…”

In Vitro Susceptibilities of Shigella flexneri and Streptococcus pyogenes to Inner Gel of Aloe barbadensis Miller

“…To date, more than 75 active ingredients have been identified from the inner gel (1,17,19), each of which may have a range of mechanisms of action, acting synergistically or individually to explain the numerous curative properties. This has led to a trend to isolate and analyze individual ingredients in an attempt to attribute therapeutic potential to each ingredient (20,22). This has not necessarily shed light on the issue, and in particular, in vivo studies have not been able to distinguish between the antimicrobial effects of the plant and stimulation of the immune system.…”

“…Although the activities of the AV and Aloe Vera Gel appear to be low in comparison to those of NAL and AMP, individual components may have greater activity. Glycosides of anthraquinones and dihydroxyanthraquinones (3,22), acemannan (5), and saponins (1,17) are active antibacterial components found in the whole plant and known to be constituents of the inner gel. Therefore, further research on these and other molecules will provide further evidence of the therapeutic potential of the inner gel.…”

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“…Acemannan, a polysaccharide component from whole plant material, has been proposed to have indirect antimicrobial activity through its ability to stimulate phagocytic leukocytes 113 . Effect of the anthraquinone aloe emodin on arylamine N-acetyl transferase activity in Helicobacter pylori have also been reported, showing its antimicrobial activity 114 .…”