Aloe-emodin as antiproliferative and differentiating agent on human U937 monoblastic leukemia cells

Tabolacci, Claudio; Oliverio, Serafina; Lentini, Alessandro; Rossi, Stefania; Galbiati, Alice; Montesano, Carla; Mattioli, Palma; Provenzano, Bruno; Facchiano, Francesco; Beninati, Simone

doi:10.1016/j.lfs.2011.09.008

Cited by 37 publications

(19 citation statements)

References 52 publications

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“…In the present study, we found that Aloe emodin significantly inhibits U87 cell proliferation in dose-dependent and time-dependent manners. This result is consistent with previous reports on inhibitory effects of Aloe emodin treatment in various human cancer cells such as hepatoma cells (Kuo et al, 2002), skin cancers (Wasserman et al, 2002), gastric cancers (Qin et al, 2010), leukaemic cells (Tabolacci et al, 2011) and breast cancer cells (Huang et al, 2013). Our study also demonstrated that the IC 50 at 24 hours of Aloe emodin treatment in U87 cells was much higher as compared to other human cancer cells such as lung squamous cancers (Lee, 2001), liver cancers (Kuo et al, 2002) and tongue squamous cancers (Chiu et al, 2009).…”

Section: Discussionsupporting

confidence: 93%

Expression Profile of Genes Modulated by Aloe emodin in Human U87 Glioblastoma Cells

Haris

Ismail²,

Idris³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Glioblastoma, the most aggressive and malignant form of glioma, appears to be resistant to various chemotherapeutic agents. Hence, approaches have been intensively investigated to targeti specific molecular pathways involved in glioblastoma development and progression. Aloe emodin is believed to modulate the expression of several genes in cancer cells. We aimed to understand the molecular mechanisms underlying the therapeutic effect of Aloe emodin on gene expression profiles in the human U87 glioblastoma cell line utilizing microarray technology. The gene expression analysis revealed that a total of 8,226 gene alterations out of 28,869 genes were detected after treatment with 58.6 µg/ml for 24 hours. Out of this total, 34 genes demonstrated statistically significant change (p<0.05) ranging from 1.07 to 1.87 fold. The results revealed that 22 genes were up-regulated and 12 genes were down-regulated in response to Aloe emodin treatment. These genes were then grouped into several clusters based on their biological functions, revealing induction of expression of genes involved in apoptosis (programmed cell death) and tissue remodelling in U87 cells (p<0.01). Several genes with significant changes of the expression level e.g. SHARPIN, BCAP31, FIS1, RAC1 and TGM2 from the apoptotic cluster were confirmed by quantitative real-time PCR (qRT-PCR). These results could serve as guidance for further studies in order to discover molecular targets for the cancer therapy based on Aloe emodin treatment.

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Section: Discussionsupporting

confidence: 93%

Expression Profile of Genes Modulated by Aloe emodin in Human U87 Glioblastoma Cells

Haris

Ismail²,

Idris³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Since systemic administration of IL-2 or IL-12, in cancer immunotherapy, induces severe side effects, the local stimulation of these cytokine by AE in the tumor microenvironment appears particularly promising for future anticancer strategies. This hypothesis is corroborated by our previous work, in which we demonstrated that AE is able to induce the production of anticancer cytokines, such as IL-12, also in U937 leukemia cells (Tabolacci et al, 2011). Interestingly, it has been demonstrated that the serum levels of IL-12 and IFN-γ in TG2 À / À mice are significantly decreased (Falasca et al, 2005), as well as in dendritic cells after the inhibition of TG2 cross-linking activity (Matic et al, 2010).…”

Section: Discussionsupporting

confidence: 87%

“…For intracellular TG activity, melanoma cells plated on 100-mm Petri dishes (1 Â 10 6 ) were grown in the presence of [ 14 C]-methylamine (0.5 μl/ml complete culture medium) either in the absence or in the presence of 30 μM AE. Radiolabelled amine incorporation into cell protein was measured with a scintillation counter (Beckman LS-5000TD, Fullerton, CA, USA) and transamidating activity expressed as reported elsewhere (Tabolacci et al, 2011).…”

Section: Determination Of Melanin Content Intracellular Protoporphyrmentioning

confidence: 99%

Aloe-emodin exerts a potent anticancer and immunomodulatory activity on BRAF-mutated human melanoma cells

Tabolacci

Cordella

Turcano

et al. 2015

European Journal of Pharmacology

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“…Aloe-emodin has anti-proliferative effects and induces cellular apoptosis (27)(28)(29)(30). Aloe-emodin has anti-cancer activity in neuroectodermal tumors (31), nasopharyngeal carcinoma (32), lung squamous cell carcinoma (33), hepatoma cells (34), gastric cancer (35) and prostate cancer (36).…”

Section: Introductionmentioning

confidence: 99%

Aloe-emodin suppresses esophageal cancer cell TE1 proliferation by inhibiting AKT and ERK phosphorylation

et al. 2016

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Abstract. Aberrant AKT and extracellular signal-regulated kinase (ERK) activation is often observed in various human cancers. Both AKT and ERK are important in the phosphoinositide 3-kinase/AKT and mitogen-activated protein kinase kinase/ERK signaling pathways, which play vital roles in cell proliferation, differentiation and survival. Compounds that are able to block these pathways have therefore a promising use in cancer treatment and prevention. The present study revealed that AKT and ERK are activated in esophageal cancer TE1 cells. Aloe-emodin, an anthraquinone present in aloe latex, can suppress TE1 cell proliferation and anchor-independent cell growth. Aloe-emodin can also reduce the number of TE1 cells in S phase. Protein analysis indicated that aloe-emodin inhibits the phosphorylation of AKT and ERK in a dose-dependent manner. Overall, the present data indicate that aloe-emodin can suppress TE1 cell growth by inhibiting AKT and ERK phosphorylation, and suggest its clinical use for cancer therapy.

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Aloe-emodin as antiproliferative and differentiating agent on human U937 monoblastic leukemia cells

Cited by 37 publications

References 52 publications

Expression Profile of Genes Modulated by Aloe emodin in Human U87 Glioblastoma Cells

Expression Profile of Genes Modulated by Aloe emodin in Human U87 Glioblastoma Cells

Aloe-emodin exerts a potent anticancer and immunomodulatory activity on BRAF-mutated human melanoma cells

Aloe-emodin suppresses esophageal cancer cell TE1 proliferation by inhibiting AKT and ERK phosphorylation

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