Aloe arborescens juice prevents EMF-induced oxidative stress and thus protects from pathophysiology in the male reproductive system in vitro

Sołek, Przemysław; Majchrowicz, Lena; Koziorowski, Marek

doi:10.1016/j.envres.2018.05.035

Cited by 17 publications

(12 citation statements)

References 54 publications

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“…According to recent data reported here [ 122 , 146 , 148 ], the strict link between EMF-related damage of reproductive systems and oxidative stress is reinforced by the observations of protective effects of antioxidant supplementations that require to be confirmed in humans. This approach, although still unexplored in the female reproductive system, could represent an important preventive strategy.…”

Section: Conclusion and Final Remarksmentioning

confidence: 55%

“…Exposure of in vitro mouse spermatogenesis pathway cell lines (GC-1 spg and GC-2 spd) to 50 or 120 Hz ELF-MF determined an increase in superoxide anion production, which was associated to activation of the intrinsic apoptotic pathway and proliferation inhibition, although after a 48-hour postexposure recovery, an increased activation of NF- κ B, a transcription factor involved in the maintenance of redox balance, was found [ 147 , 148 ]. These effects were abolished by medium supplementation with Aloe arborescens's juice [ 148 ].…”

Section: Emf Effects In Male Reproductive System and Fertilitymentioning

confidence: 99%

“…Conversely, Iorio et al [ 161 ] gave evidence for a stimulatory effect on human sperm motility exerted by a 50 Hz, 5 mT ELF-MF exposure in association with a greater activity of mitochondrial metabolism efficiency. On the other hand, Solek et al [ 148 ] reported no significant effects of EMF exposure (50 Hz, 2.5 mT, for 2 h) on mitochondrial function in mouse germ cells.…”

Section: Emf Effects In Male Reproductive System and Fertilitymentioning

confidence: 99%

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Role of Mitochondria in the Oxidative Stress Induced by Electromagnetic Fields: Focus on Reproductive Systems

Santini

Cordone

Falone

et al. 2018

Oxidative Medicine and Cellular Longevity

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Modern technologies relying on wireless communication systems have brought increasing levels of electromagnetic field (EMF) exposure. This increased research interest in the effects of these radiations on human health. There is compelling evidence that EMFs affect cell physiology by altering redox-related processes. Considering the importance of redox milieu in the biological competence of oocyte and sperm, we reviewed the existing literature regarding the effects of EMFs on reproductive systems. Given the role of mitochondria as the main source of reactive oxygen species (ROS), we focused on the hypothesis of a mitochondrial basis of EMF-induced reproductive toxicity. MEDLINE, Web of Science, and Scopus database were examined for peer-reviewed original articles by searching for the following keywords: “extremely low frequency electromagnetic fields (ELF-EMFs),” “radiofrequency (RF),” “microwaves,” “Wi-Fi,” “mobile phone,” “oxidative stress,” “mitochondria,” “fertility,” “sperm,” “testis,” “oocyte,” “ovarian follicle,” and “embryo.” These keywords were combined with other search phrases relevant to the topic. Although we reported contradictory data due to lack of uniformity in the experimental designs, a growing body of evidence suggests that EMF exposure during spermatogenesis induces increased ROS production associated with decreased ROS scavenging activity. Numerous studies revealed the detrimental effects of EMFs from mobile phones, laptops, and other electric devices on sperm quality and provide evidence for extensive electron leakage from the mitochondrial electron transport chain as the main cause of EMF damage. In female reproductive systems, the contribution of oxidative stress to EMF-induced damages and the evidence of mitochondrial origin of ROS overproduction are reported, as well. In conclusion, mitochondria seem to play an important role as source of ROS in both male and female reproductive systems under EMF exposure. Future and more standardized studies are required for a better understanding of molecular mechanisms underlying EMF potential challenge to our reproductive system in order to improve preventive strategies.

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Section: Conclusion and Final Remarksmentioning

confidence: 55%

Section: Emf Effects In Male Reproductive System and Fertilitymentioning

confidence: 99%

Section: Emf Effects In Male Reproductive System and Fertilitymentioning

confidence: 99%

See 1 more Smart Citation

Role of Mitochondria in the Oxidative Stress Induced by Electromagnetic Fields: Focus on Reproductive Systems

Santini

Cordone

Falone

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…For the MTT assay, a standard protocol reported in the previous study was employed [20]. After 48 h incubation with or without LPS, the cell culture medium was replaced with medium supplemented with MTT (0.5 mg/mL).…”

Section: Mtt Assaymentioning

confidence: 99%

Focus on the Role of Klotho Protein in Neuro-Immune Interactions in HT-22 Cells Upon LPS Stimulation

Rusinek

Sołek

Tabęcka-Łonczyńska

et al. 2020

Cells

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Neuroinflammation is defined as the activation of the brain’s innate immune system in response to an inflammatory challenge and is considered to be a prominent feature of neurodegenerative diseases. The contribution of overactivated neuroglial cells to neuroinflammation and neurodegenerative disorders is well documented, however, the role of hippocampal neurons in the neuroinflammatory process remains fragmentary. In this study, we show for the first time, that klotho acts as a signal transducer between pro-survival and pro-apoptotic crosstalk mediated by ER stress in HT-22 hippocampal neuronal cells during LPS challenge. In control HT-22 cells, LPS treatment results in activation of the IRE1α-p38 MAPK pathway leading to increased secretion of anti-inflammatory IL-10, and thus, providing adaptation mechanism. On the other hand, in klotho-deficient HT-22 cells, LPS induces oxi-nitrosative stress and genomic instability associated with telomere dysfunctions leading to p53/p21-mediated cell cycle arrest and, in consequence, to ER stress, inflammation as well as of apoptotic cell death. Therefore, these results indicate that klotho serves as a part of the cellular defense mechanism engaged in the protection of neuronal cells against LPS-mediated neuroinflammation, emerging issues linked with neurodegenerative disorders.

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“…Proteins of interest were visualized by Western Blotting according to Solek et al 2018(Solek et al 2018a).…”

Section: Western Blottingmentioning

confidence: 99%

Wild Medicinal Fungi: Phallus Impudicus-induced Telomere Damage Triggers p21/p53 and p16-dependent Cell Cycle Arrest and Drives to Male Fertility Reduction in Vitro

Sołek¹,

Shemedyuk

et al. 2020

Preprint

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The multifunctional characteristics of Phallus impudicus extract encourages to conduct research for its probable potential in medical applications. Well, science is constantly seeking new evidence for the biological activity of extracts of natural origin. Drugs of natural origin should not cause any side effects on the physiological functions of the human body; however, this is not always successful. In this study, we used in vitro approach to evaluate the toxicity of alcohol Phallus impudicus extract on spermatogenic cells. We show, for the first time, cytotoxic properties of Phallus impudicus treatment associated with a decrease in cellular metabolic activity, dysregulation of redox homeostasis and impairment of selected antioxidant cell protection systems. As a consequence, p53/p21- and p16-mediated cell cycle arrest followed by p-27 activation is initiated. The observed changes were associated with telomere shortening and numerous DNA damage at the chromosome ends (altered expression pattern of TRF1 and TRF2 proteins), as well as upregulation of cleaved caspase-3 with a decrease in Bcl-2 synthesis, suggesting induction of apoptotic death. Therefore, these results provide molecular evidence for mechanistic pathways and novel adverse outcomes linked to the Phallus impudicus treatment towards men's health and fertility reduction.

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Aloe arborescens juice prevents EMF-induced oxidative stress and thus protects from pathophysiology in the male reproductive system in vitro

Cited by 17 publications

References 54 publications

Role of Mitochondria in the Oxidative Stress Induced by Electromagnetic Fields: Focus on Reproductive Systems

Role of Mitochondria in the Oxidative Stress Induced by Electromagnetic Fields: Focus on Reproductive Systems

Focus on the Role of Klotho Protein in Neuro-Immune Interactions in HT-22 Cells Upon LPS Stimulation

Wild Medicinal Fungi: Phallus Impudicus-induced Telomere Damage Triggers p21/p53 and p16-dependent Cell Cycle Arrest and Drives to Male Fertility Reduction in Vitro

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