“…If Ag + alone is responsible for phytotoxicity, then the accumulation of equal quantities of Ag in plants would be expected to induce comparable changes in gene expression and physiological functions. Though similar experiments have been reported previously [ 3 , 10 , 11 , 13 , [25] , [26] , [27] ], our approach differs in that we ensured similar in planta Ag concentrations in both nanosilver and Ag + treatments, and that both our transcriptomic and physiological data were generated from independently repeated experiments as opposed to biological replicates.…”