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Staats, N.; Deckere, E.M.G.T. de; Winder, Ben de; Stal, Lucas J.

doi:10.1023/a:1017545204214

Cited by 31 publications

(9 citation statements)

References 36 publications

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“…The 'attached exopolysaccharides' or 'bound EPS' extracted with warm water from cultured C. closterium and Nitzschia sp. showed consistent patterns of accumulation in the light and depletion in the dark (Staats et al, 2000a;. By contrast, in these studies, there was little variation with light or dark incubation in the unbound EPS or the nominal 'intracellular' carbohydrates, which were extracted from the cell pellets left after warm water treatment.…”

Section: Discussionmentioning

confidence: 57%

“…By contrast, in these studies, there was little variation with light or dark incubation in the unbound EPS or the nominal 'intracellular' carbohydrates, which were extracted from the cell pellets left after warm water treatment. DCMU, an inhibitor of photosystem II, also inhibited accumulation of the putative 'attached exopolysaccharide' of C. closterium (Staats et al, 2000a). Glc was the dominant component of the warm water extracts of C. closterium and Nitzschia sp.…”

Section: Discussionmentioning

confidence: 95%

“…It was suggested in one study (Staats et al, 2000a) that chrysolaminaran could not contribute to the EPS fraction extracted by warm water in which the polysaccharides were recovered by alcohol precipitation because chrysolaminaran does not precipitate in cold ethanol. However, the chrysolaminaran of P. tricornutum was recovered by precipitation with ca.…”

Section: Discussionmentioning

confidence: 99%

“…During the last five years, there have been a number of studies that have adopted a warm water treatment to extract putative extracellular polysaccharides from marine benthic diatoms (Staats et al, 1999(Staats et al, , 2000ade Brouwer & Stal, 2001. In these studies, the basic protocol first involved the separation by centrifugation of cells from the surrounding medium, which contained what was typically described as the 'unbound' or 'unattached' EPS.…”

mentioning

confidence: 99%

“…In these studies, the basic protocol first involved the separation by centrifugation of cells from the surrounding medium, which contained what was typically described as the 'unbound' or 'unattached' EPS. The pelleted cells were resuspended and extracted with tap or distilled water at 308C for 1 h. Centrifugation and recovery of the supernatant gave a fraction described as 'colloidal carbohydrates ' (de Brouwer & Stal, 2001), 'exopolysaccharides' (Staats et al, 2000b), 'attached exopolysaccharides' (Staats et al, 2000a), 'attached EPS' (Staats et al, 1999;, or 'bound EPS' . In studies of cultured diatom species, where the carbohydrate composition of the nominal 'attached exopolysaccharides' or 'bound EPS' was reported, glucose was invariably the dominant component (55 -85% of total monosaccharides, Staats et al, 1999;.…”

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confidence: 99%

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The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides

Chiovitti

Molino

Crawford

et al. 2004

European Journal of Phycology

104

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Several recent studies have employed warm-water treatment of diatom cells to extract nominally bound extracellular polymeric substances. Where examined, the dominant neutral sugar in these extracts was glucose. In the present study, we sought to characterize the structure of the glucose-rich polymers in the water extracts of diatoms and to determine the origin of these polymers. The marine diatoms surveyed were Phaeodactylum tricornutum, Cylindrotheca fusiformis, Craspedostauros australis and Thalassiosira pseudonana. A freshwater species, Pinnularia viridis, was also investigated for the dye labelling experiments. Freshly harvested marine diatoms were extracted with water at 308C for 1 h. Constituent monosaccharide analyses showed that glucose was the dominant neutral sugar (80 -95 mol% of the total) in the extracts from three marine species, whereas the P. tricornutum extract contained predominantly ribose, galactose and glucose, and was inferred to be enriched in low-molecular-weight components. Linkage analysis of the constituent monosaccharides and proton nuclear magnetic resonance spectroscopy showed that the glucose in these extracts was derived primarily from 1,3-b-D-glucan. Immunocytochemistry with a monoclonal anti-1,3-b-D-glucan antibody confirmed that the glucan was localized in the vacuoles of diatom cells preserved by freeze-substitution. Nearly all diatom cells incubated with a fluorescent dye, DiBAC 4 (3), during warm water treatment at 308C or 608C incorporated the dye, demonstrating that the membrane integrity of the diatoms was compromised and supporting the contention that intracellular glucan was released during the treatment. In light of these data, the extracellular glucans of diatoms reported in some previous studies are re-interpreted as intracellular chrysolaminaran.

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Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides

Chiovitti

Molino

Crawford

et al. 2004

European Journal of Phycology

104

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The effect of long‐term aerial exposure on intertidal mudflat erodibility

Nguyen

Bryan

Pilditch

2020

Earth Surf Processes Landf

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Intertidal zones by definition are exposed to air at low tide, and the exposure duration can be weeks (e.g. during neap tides) depending on water level and bed elevation. Here we investigated the effect of varying exposure duration (6h to 10 days) on intertidal mudflat erosion (measured using the EROMES device), where the effects of water content and biofilm biomass (using chlorophyll-a content as a proxy, Chl-a μgg À1) were taken into account. Sediments were collected between spring and summer

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Recent Change—North Sea

Huthnance

Weisse

Wahl

et al. 2016

North Sea Region Climate Change Assessment

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This chapter discusses past and ongoing change in the following physical variables within the North Sea: temperature, salinity and stratification; currents and circulation; mean sea level; and extreme sea levels. Also considered are carbon dioxide; pH and nutrients; oxygen; suspended particulate matter and turbidity; coastal erosion, sedimentation and morphology; and sea ice. The distinctive character of the Wadden Sea is addressed, with a particular focus on nutrients and sediments. This chapter covers the past 200 years and focuses on the historical development of evidence (measurements, process understanding and models), the form, duration and accuracy of the evidence available, and what the evidence shows in terms of the state and trends in the respective variables. Much work has focused on detecting long-term change in the North Sea region, either from measurements or with models. Attempts to attribute such changes to, for example, anthropogenic forcing are still missing for the North Sea. Studies are urgently needed to assess consistency between observed changes and current expectations, in order to increase the level of confidence in projections of expected future conditions.

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Cited by 31 publications

References 36 publications

The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides

The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides

The effect of long‐term aerial exposure on intertidal mudflat erodibility

Recent Change—North Sea

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