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Bethke, Paul C.; Fath, Angelika; Spiegel, Yoav N.; Hwang, Yong-sic; Jones, Russell L.

doi:10.1023/a:1019659319630

Cited by 29 publications

(4 citation statements)

References 45 publications

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“…In rice, the accumulation of gibberellins increases the resistance to necrotrophs and the susceptibility to (hemi)biotrophs [ 116 ]. Interestingly, the opposite effects were observed in wheat and barley [ 117 ]. In our experiments, the metabolites in the gibberellin biosynthesis module mainly accumulated in response to both types of Prs strains, suggestive of the complex effects of gibberellins on rye immune responses.…”

Section: Discussionmentioning

confidence: 99%

Leaf rust (Puccinia recondita f. sp. secalis) triggers substantial changes in rye (Secale cereale L.) at the transcriptome and metabolome levels

Krępski,

Piasecka,

Święcicka

et al. 2024

BMC Plant Biol

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Background Rye (Secale cereale L.) is a cereal crop highly tolerant to environmental stresses, including abiotic and biotic stresses (e.g., fungal diseases). Among these fungal diseases, leaf rust (LR) is a major threat to rye production. Despite extensive research, the genetic basis of the rye immune response to LR remains unclear. Results An RNA-seq analysis was conducted to examine the immune response of three unrelated rye inbred lines (D33, D39, and L318) infected with compatible and incompatible Puccinia recondita f. sp. secalis (Prs) isolates. In total, 877 unique differentially expressed genes (DEGs) were identified at 20 and 36 h post-treatment (hpt). Most of the DEGs were up-regulated. Two lines (D39 and L318) had more up-regulated genes than down-regulated genes, whereas the opposite trend was observed for line D33. The functional classification of the DEGs helped identify the largest gene groups regulated by LR. Notably, these groups included several DEGs encoding cytochrome P450, receptor-like kinases, methylesterases, pathogenesis-related protein-1, xyloglucan endotransglucosylases/hydrolases, and peroxidases. The metabolomic response was highly conserved among the genotypes, with line D33 displaying the most genotype-specific changes in secondary metabolites. The effect of pathogen compatibility on metabolomic changes was less than the effects of the time-points and genotypes. Accordingly, the secondary metabolome of rye is altered by the recognition of the pathogen rather than by a successful infection. The results of the enrichment analysis of the DEGs and differentially accumulated metabolites (DAMs) reflected the involvement of phenylpropanoid and diterpenoid biosynthesis as well as thiamine metabolism in the rye immune response. Conclusion Our work provides novel insights into the genetic and metabolic responses of rye to LR. Numerous immune response-related DEGs and DAMs were identified, thereby clarifying the mechanisms underlying the rye response to compatible and incompatible Prs isolates during the early stages of LR development. The integration of transcriptomic and metabolomic analyses elucidated the contributions of phenylpropanoid biosynthesis and flavonoid pathways to the rye immune response to Prs. This combined analysis of omics data provides valuable insights relevant for future research conducted to enhance rye resistance to LR.

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Section: Discussionmentioning

confidence: 99%

Leaf rust (Puccinia recondita f. sp. secalis) triggers substantial changes in rye (Secale cereale L.) at the transcriptome and metabolome levels

Krępski,

Piasecka,

Święcicka

et al. 2024

BMC Plant Biol

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“…(Uemura et al, 2002; Zheng et al, 2017; Zhang et al, 2020). Moreover, abscisic acid (ABA) accumulates in the integument layers to delay aleurone vacuolation and induce seed dormancy (Bethke et al, 1999; Bethke et al, 2002; Bethke et al, 2007), whereas gibberellins (GA) promoted vacuolation to mobilize the reserve enzymes and compounds to incite aleurone PCD (Bethke et al, 2002; Zhang et al, 2020). Interestingly, the seed coat‐type VPE (δVPE) has been identified to control PCD in the inner integument layers during seed development, suggesting that δVPE might be involved in a GA response in the inner integument cells during seed development.…”

Section: Biological Function Of Vacuolar Processing Enzyme In Regulat...mentioning

confidence: 99%

Unraveling the Molecular Functions of Multifaced Plant‐Vacuolar Processing Enzymes

Sarwar,

Yu,

Zekraoui

et al. 2024

Physiologia Plantarum

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In the plant development cycle, different types of proteases play essential functions. Plant vacuolar processing enzymes (VPEs) are a group of cysteine proteases localized in the cell vacuoles that process and regulate several larger proprotein precursors. VPEs activate the proteolytic cleavage of proproteins precursors within aspartic acid and asparagine residues to trigger the tightly controlled programmed cell death process induced by different stages of plant development and environmental cues, impacting myriad plant physiological processes. In plants, VPEs are categorized into embryo, vegetative, and seed coat‐types, in which embryo and seed coat‐type VPEs are mainly involved in processing the seed storage protein precursors to provide nutrients for early growth, while the vegetative type induces programmed cell death in a varied range of vegetative tissues. Recently, several plant VPEs exhibited transpeptidase activity, which is essential for the cyclization mechanism during the production of cyclotides. In this review, we explore and understand the complex functions of plant VPEs, which can lead to the production of stress‐tolerant and higher‐yield crops, and provide the recent advances in the application of VPEs in molecular pharming, which highlights the significant implications of VPEs in protein science and agriculture.

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“…GA-triggering enzymes initiate a program that culminates in CD, while ABA seems to prevent CD. However, GA sensitizes aleurone cells to ROS, a regulator of cell viability 17 .…”

Section: Introductionmentioning

confidence: 99%

Hormonal crosstalk controls cell death induced by kinetin in roots of Vicia faba ssp. minor seedlings

Kaźmierczak

Tarkowská

Plačková

et al. 2023

Sci Rep

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Studies of vitality/mortality of cortex cells, as well as of the concentrations of ethylene (ETH), gibberellins (GAs), indolic compounds/auxins (ICs/AUXs) and cytokinins (CKs), were undertaken to explain the hormonal background of kinetin (Kin)-regulated cell death (RCD), which is induced in the cortex of the apical parts of roots of faba bean (Vicia faba ssp. minor) seedlings. Quantification was carried out with fluorescence microscopy, ETH sensors, spectrophotometry and ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC‒MS/MS). The results indicated that Kin was metabolized to the transport form, i.e., kinetin-9-glucoside (Kin9G) and kinetin riboside (KinR). KinR was then converted to cis-zeatin (cZ) in apical parts of roots with meristems, to cis-zeatin riboside (cZR) in apical parts of roots without meristems and finally to cis-zeatin riboside 5’-monophosphate (cZR5’MP), which is indicated to be a ligand of cytokinin-dependent receptors inducing CD. The process may be enhanced by an increase in the amount of dihydrozeatin riboside (DHZR) as a byproduct of the pathway of zeatin metabolism. It seems that crosstalk of ETH, ICs/AUXs, GAs and CKs with the cZR5’MP, the cis-zeatin-dependent pathway, but not the trans-zeatin-dependent pathway, is responsible for Kin-RCD, indicating that the process is very specific and offers a useful model for studies of CD hallmarks in plants.

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Cited by 29 publications

References 45 publications

Leaf rust (Puccinia recondita f. sp. secalis) triggers substantial changes in rye (Secale cereale L.) at the transcriptome and metabolome levels

Leaf rust (Puccinia recondita f. sp. secalis) triggers substantial changes in rye (Secale cereale L.) at the transcriptome and metabolome levels

Unraveling the Molecular Functions of Multifaced Plant‐Vacuolar Processing Enzymes

Hormonal crosstalk controls cell death induced by kinetin in roots of Vicia faba ssp. minor seedlings

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