Allotypes of Light Chains of Rat Immunoglobulins and Their Application to the Study of Antibody Biosynthesis

Nezlin, Roald; Rokhlin, O.V.

doi:10.1007/978-1-4684-8142-6_6

Cited by 14 publications

(3 citation statements)

References 41 publications

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“…The legend to Figure I summarises the frequencies of these four populations. Overall (though not in the particular experiment illustrated) Igk-Ib-positive cells predominated slightly over Igkl-positives (mean 49Gb against 43% among B cells) as expected from earlier studies of allelic preference (Nezlin and Rokhlin, 1976;Hunt and Williams, 1974). In both the F1 and the chimera the single expressors exhibited very low levels of the other allotype, which at least in the chimera can only have been passively acquired: for instance, the cells stained red were also slightly green, to a greater extent than the T cells.…”

Section: Resultssupporting

confidence: 77%

Allelic exclusion in rat kappa immunoglobulin chains: extent of Jk rearrangement in normal B lymphocytes.

Tsukamoto¹,

Hunt²

1984

The EMBO Journal

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The frequency of normal rat peripheral B lymphocytes stained for surface immunoglobulin kappa allotypes a and b in (a X b) F1 heterozygotes was assessed by two‐colour immunofluorescence on a fluorescence‐activated cell sorter. The upper limit to the frequency of double‐ stainers was 8% among all kappa‐positive cells, though it was not resolved how far cytophilic antibody accounted for these. Cells expressing each allotype singly were isolated and the extent of rearrangement of the genes encoding the joining‐kappa segment on the expressed and non‐expressed chromosome were independently assessed. The expressed allele was found to be virtually completely rearranged while the non‐expressed allele showed approximately 45‐60% rearrangement. The implications of this substantial non‐productive rearrangement for models of allelic exclusion are discussed.

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Section: Resultssupporting

confidence: 77%

Allelic exclusion in rat kappa immunoglobulin chains: extent of Jk rearrangement in normal B lymphocytes.

Tsukamoto¹,

Hunt²

1984

The EMBO Journal

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“…The structure of IgG subtypes and allotypes has been extensively studied for more than a half century, and the differences have been illustrated in other literatures, with the different regions and various components of IgG. [17][18][19] Schematics for the structure of other immunoglobins such as IgE, IgD, IgA, and IgM have also been provided in literature. 17,[19][20][21] This detailed understanding of the structure of immunoglobulins has led to the development of therapies such as monoclonal antibodies, which target specific antigens and have shown promising results in the treatment of various diseases.…”

Section: Igg Basicsmentioning

confidence: 99%

Novel Electrochemical Biosensing Strategy for Antibody IgG detections

Patel¹,

Sharma²

2023

Preprint

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The human immune system is a complex network of cells, tissues, and organs that work together to protect the body from harmful pathogens. Antibodies, also known as immunoglobulins (Ig), are small proteins that play a vital role in the immune system's defense mechanism. Among the five classes of immunoglobulins, Immunoglobin G (IgG) is the most abundant and widely studied. This article provides an in-depth overview of the basics of IgG, including their chemical and physical properties and their roles in the human immune system. The article then focuses on the critical biosensor working principles with an emphasis on electrochemical biosensors. Biosensors are analytical devices that convert a biological response into an electrical signal, allowing for rapid and sensitive detection of specific analytes. The use of biosensors for IgG detection has gained significant attention in recent years due to their sensitivity, specificity, and ability to detect IgG in real-time. This article also discusses many novel strategies that have been reported in the literature for sensitive IgG detection. These strategies include the use of different biorecognition elements, such as antibodies, aptamers, enzymes, and biomimetic materials. Moreover, the article concludes by highlighting recent research advances and future directions for sensitive IgG detection, such as the use of nanomaterials and adaptable machine learning models, leading to a more efficient method of IgG detection.

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“…If the antibodies are radioiodinated, all the procedure can be performed analytically. The fractions obtained could be used for further investigations, for example, for identification of their allotypes (22).…”

Section: ·mentioning

confidence: 99%

Quantitation and purification of antibodies and antigens by immunoadsorbents

Nezlin¹

1979

Pure and Applied Chemistry

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-For the last two decades immunoadaorption technique haa been widely used for quantitation and preparative isolation of antibodies and antigena. The procedure usually involves immobili~ation of antibodies or antigens (ligands), adeorption of counter-ligands,washing from nonapecific proteina, and elution. For preparation of immunoadsorbenta, the ligands are fixed on insoluble supporta, crosa-linked by bifunctional reagents or mechanically entrapped in inert polymere •. Two types of carriers are often uaed: agarose in a beaded form {Sepharoae) and small celluloae particlea. The immunoadaorbents prepared on theae matricea are atable, have high capacity andminimum nonapecific adaorption. The ligands fixed on the small cellulose particles are tightly packed and prove to be uaeful for the batch erperimenta. The beaded agarose possesses good flow characteristics and is very efficient for the isolation of one or another Counterpart of the antigen-antibody reaction by column technique. The fi~ed antigens have been recently used for specific fractionation of lymphoid cells. In such experiments the antigens fixed on nylon fibers are especially effective.

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Allotypes of Light Chains of Rat Immunoglobulins and Their Application to the Study of Antibody Biosynthesis

Cited by 14 publications

References 41 publications

Allelic exclusion in rat kappa immunoglobulin chains: extent of Jk rearrangement in normal B lymphocytes.

Allelic exclusion in rat kappa immunoglobulin chains: extent of Jk rearrangement in normal B lymphocytes.

Novel Electrochemical Biosensing Strategy for Antibody IgG detections

Quantitation and purification of antibodies and antigens by immunoadsorbents

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