Allotransplantation of adult spinal cord tissues after complete transected spinal cord injury: Long-term survival and functional recovery in canines

Shen, He; Wu, Shu-Yu; Chen, Xi; Bai, Xu; Ma, Dezun; Zhao, Yannan; Zhuang, Yan; Chen, Bing; Hou, Xun; Li, Jiayin; Cao, Yudong; Fu, Xianyong; Tan, Jun; Yin, Weiqiang; Li, Juan; Li, Meng; Shi, Yang; Xiao, Zhifeng; Jiang, Xingjun

doi:10.1007/s11427-019-1623-5

Cited by 9 publications

(5 citation statements)

References 32 publications

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“…All animal procedures were performed depending on the Guide for the Care and Use of Laboratory Animals from the National Institutes of Health. The surgical procedure was similar to that previously described, with minor modifications (Han et al, 2018;Shen et al, 2019;Shen et al, 2020). Mice were anesthetized through administration of chloral hydrate (0.5 g kg −1…”

Section: Surgical Proceduresmentioning

confidence: 99%

Lineage tracing reveals the origin of Nestin-positive cells are heterogeneous and rarely from ependymal cells after spinal cord injury

Xue

Shu

Xiao

et al. 2021

Sci. China Life Sci.

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Nestin is expressed extensively in neural stem/progenitor cells during neural development, but its expression is mainly restricted to the ependymal cells in the adult spinal cord. After spinal cord injury (SCI), Nestin expression is reactivated and Nestinpositive (Nestin + ) cells aggregate at the injury site. However, the derivation of Nestin + cells is not clearly defined. Here, we found that Nestin expression was substantially increased in the lesion edge and lesion core after SCI. Using a tamoxifen inducible CreER(T2)-loxP system, we verified that ependymal cells contribute few Nestin + cells either to the lesion core or the lesion edge after SCI. In the lesion edge, GFAP + astrocytes were the main cell type that expressed Nestin; they then formed an astrocyte scar.In the lesion core, Nestin + cells expressed αSMA or Desmin, indicating that they might be derived from pericytes. Our results reveal that Nestin + cells in the lesion core and edge came from various cell types and rarely from ependymal cells after complete transected SCI, which may provide new insights into SCI repair.

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Section: Surgical Proceduresmentioning

confidence: 99%

Lineage tracing reveals the origin of Nestin-positive cells are heterogeneous and rarely from ependymal cells after spinal cord injury

Xue

Shu

Xiao

et al. 2021

Sci. China Life Sci.

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“…Transplanted aSCGs have been shown to survive long-term at lesion sites and improve functional recovery in rodent and canine SCI models. [179] A growth factor "cocktail" containing BDNF, NT-3, and VEGF was applied to the transplants, which enhanced the survival levels of the donor cells and improved the therapeutic efficiency of aSCGs. Adult spinal cord tissue grafts are composed of various types of cells, such as neurons, astrocytes, oligodendrocytes, and endothelial cells, and have a complex architecture and extracellular matrix.…”

Section: Transplantation Of Adult Spinal Cord Tissue Grafts For Compl...mentioning

confidence: 99%

“…Adult spinal cord tissue grafts are composed of various types of cells, such as neurons, astrocytes, oligodendrocytes, and endothelial cells, and have a complex architecture and extracellular matrix. They can therefore be considered as a promising approach for SCI future repairs [178][179][180] (Figure 5).…”

Section: Transplantation Of Adult Spinal Cord Tissue Grafts For Compl...mentioning

confidence: 99%

Advances in Biomaterial‐Based Spinal Cord Injury Repair

Shen

Fan

You

et al. 2021

Adv Funct Materials

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Spinal cord injury (SCI) often leads to the loss of motor and sensory functions and is a major challenge in neurological clinical practice. Understanding the pathophysiological changes and the inhibitory microenvironment is crucial to enable the identification of potential mechanisms for functional restoration and to provide guidance for the development of efficient treatment and repair strategies. To date, the implantation of specifically functionalized biomaterials in the lesion area has been shown to help promote axon regeneration and facilitate neuronal circuit generation by remolding SCI microenvironments. Moreover, structural and functional restoration of the spinal cord through the transplantation of naive spinal cord tissue grafts from adult donors, artificial spinal cord-like tissue developed from tissue engineering, and 3D printing will open up new avenues for SCI treatment. This review focuses on the dynamic pathophysiological changes in SCI microenvironments, biomaterials for SCI repairs, strategies for restoring spinal cord structure and function, experimental animal models, regenerative mechanisms, and clinical studies for SCI repair. The current status, recent advances, challenges, and prospects of scaffold-based SCI repair from basic to clinical settings are summarized and discussed, to provide a reference that will help to guide the future exploration and development of spinal cord regeneration strategies.

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“…Another excellent study by Iwashita [ 8 ] also reported that the transplanted SCT exhibited robust axonal growth and regeneration, which enabled rats walk, run and climb with almost normal hind forelimb coordination. In our previous study, we proved that transplanted adult SCT (aSCT) into SCI sites in rat and canines could promote the recovery of locomotor function [ 9 , 10 ]. At present, the main obstacle of organ/tissue transplantation is immune rejection [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Locally controlled release of immunosuppressive promotes survival of transplanted adult spinal cord tissue

Wang

Sun

et al. 2022

Regenerative Biomaterials

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Transplantation of adult spinal cord tissue (aSCT) is a promising treatment for spinal cord injury (SCI) basing on various types of neural cells and matrix components inside aSCT. However, long-term systemic administration of immunosuppressors (e.g. tacrolimus, TAC) is required for the survival of allogeneic tissue, which often associated with severe side effects such as infection, liver damage, and renal failure, etc. In this study, a triglycerol monostearate-based (TGM) TAC delivery system (e.g. TAC@TGM) with high drug loading concentration was developed, which possessed injectable properties as well as sustainable and immune-responsive drug release behaviors. In complete transected SCI model, locally injected TAC@TGM could reduce the infiltration of inflammation cells, enhance the survival of transplanted aSCT (e.g. Tuj-1+ and NF+ neurons), and promote the recovery of locomotor function. Moreover, controlled release of TAC by TAC@TGM attenuated side effects of TAC on liver and kidneys compared with traditional systemic administration. More importantly, the developed TAC@TGM system provided a facile single dose of long-term immunosuppressive effect not just for aSCT transplantation, but also for other tissue/organ and cell transplantations. Graphical abstract

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Allotransplantation of adult spinal cord tissues after complete transected spinal cord injury: Long-term survival and functional recovery in canines

Cited by 9 publications

References 32 publications

Lineage tracing reveals the origin of Nestin-positive cells are heterogeneous and rarely from ependymal cells after spinal cord injury

Lineage tracing reveals the origin of Nestin-positive cells are heterogeneous and rarely from ependymal cells after spinal cord injury

Advances in Biomaterial‐Based Spinal Cord Injury Repair

Locally controlled release of immunosuppressive promotes survival of transplanted adult spinal cord tissue

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