Allosteric nanobodies to study the interactions between SOS1 and RAS
Baptiste Fischer,
Tomasz Uchański,
Aidana Sheryazdanova
et al.
Abstract:Protein-protein interactions (PPIs) are central in cell metabolism but research tools for the structural and functional characterization of these PPIs are often missing. Here we introduce broadly applicable immunization (Cross-link PPIs and immunize llamas, ChILL) and selection strategies (Display and co-selection, DisCO) for the discovery of diverse nanobodies that either stabilize or disrupt PPIs in a single experiment. We apply ChILL and DisCO to identify competitive, connective, or fully allosteric nanobod… Show more
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