Alloreactive Cytotoxic T Lymphocytes Lyse Syngeneic Influenza‐Infected Tumour Cell Targets

Abstract

Spleen cells from C57BL/10(H-2b) mice, when stimulated in vitro to Kk alloantigens, lysed syngeneic influenza A virus-infected tumour cell targets but not uninfected or vaccinia- or Sendai virus-infected targets. Peritoneal macrophage targets infected with influenza virus were not lysed. Lysis of H-2k targets and EL4-A influenza virus-infected targets was abrogated by treatment of effectors with anti-Thy 1.2 plus C and anti-Lyt 2 plus C but not by anti-Lyt 1 plus C or anti-GM-1, a natural killer cell-specific, monoclonal antibody plus C. Cold target inhibition experiments indicated that one and the same population of Tc cells see H-2Kk and H-2b plus influenza virus. Sensitization of C57BL/10 mice to Kk in vivo did not potentiate virus clearance from lungs. The data are discussed in relation to observed Ir gene effects and variation and modulation of H-2 antigens on tumour cells.

CD8 memory T cells: cross-reactivity and heterologous immunity

“…This cross-reactivity between LCMVspecific T cells and allo-antigens is consistent with earlier studies in which long-term murine CD8 T cell clones specific for either influenza virus or vesicular stomatitis virus (VSV), and human clones specific for EBV recognized allogeneic cells in cytotoxicity assays [61,62,66,67]. In addition, allo-reactive T cells specific for H2K k could be shown to lyse syngeneic H2 b -target cells infected with influenza virus [68]. Cross-reactivity between virus-specific CD8 T cells and allo-antigens has been visualized directly from mice acutely infected with LCMV [63].…”

Target Cell Lysis by Natural Killer Cells is Influenced by β2‐Microglobulin Expression

“…Ptjenotyping of effeclor cells. NK and ailoreactivc Tĉ ells were treated wilh antibody or antiserum plus complement (C) as described [14]. The antisera used were: monoclonal anti-Thy 1.2 (F7D5 clone.…”

Activated B cells can deliver help for the in vitro generation of antiviral cytotoxic T cells.

“…Furthermore, depletion of B cells in vivo affects a number of T-cell functions, such as the priming of Th cells in lymph nodes (25)(26)(27), the development of experimental allergic encephalitis (28), and the kinetics of antiviral Tc cell responses (29 (30). The method for the Tc-cell assay has been described in full (30,32).…”