Allopolyploid Origin of the Mediterranean Endemic, &lt;I&gt;Centaurium bianoris&lt;/I&gt; (Gentianaceae), Inferred by Molecular Markers

Guggisberg, Alessia; Bretagnolle, François; Mansion, Guilhem

doi:10.1600/036364406777585937

Cited by 35 publications

(46 citation statements)

References 47 publications

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“…The results of assumed ploidy level for all 4 Centaurium species included in hybridization experiments correspond with results published earlier (Zeltner, 1978;Mansion et al, 2005;Guggisberg et al, 2006). The interspecific hybrid plants would be expected to express an average nuclear DNA content of parental species after flow cytometry analysis (Meiners et al, 2011).…”

Section: Hybrid Verification By Ndna Estimationsupporting

confidence: 86%

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Interspecific in vitro hybridization in genus Centaurium and identification of hybrids via flow cytometry, RAPD, and secondary metabolite profiles

Banjanac¹,

Šiler²,

Škorić³

et al. 2014

Turk J Bot

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Most of the species from the genus Centaurium Hill readily hybridize between themselves in natural habitats. Artificial interspecific hybrid plants were obtained under in vitro conditions using reciprocal crosses between 4 species: Centaurium erythraea, C. littorale, C. maritimum, and C. pulchellum. Among 76 crossing experiments performed, 3 types of crosses produced seeds and gave 20 interspecific hybrid plants. Nuclear DNA quantification, fingerprinting of molecular markers, and determination of variation in main secondary metabolite content were employed to confirm their hybrid nature. All 3 approaches were capable of detecting hybrid plants. RAPD molecular markers proved to be the most reliable, always placing the hybrids between their parents in dendrograms, while secoiridoid glycoside content also showed intermediate profiles in the hybrid plants in relation to the parents. Hence, each of the techniques employed can be recommended for elucidating the mechanisms of interspecific hybridization in natural populations of Centaurium species in further studies. This could help to comprehend their breeding system and recognize interspecific hybridization as one of the crucial steps in polyploid formation and speciation in this genus.

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Section: Hybrid Verification By Ndna Estimationsupporting

confidence: 86%

“…On the basis of molecular data, Guggisberg et al (2006) strongly support the allopolyploid origin for C. bianoris through natural hybridization between C. maritimum (as female parent) and C. tenuiflorum (as male parent) on Majorca Island. However, the authors do not…”

Section: Significance Of the Results For The Elucidation Of Interspecmentioning

confidence: 74%

Interspecific in vitro hybridization in genus Centaurium and identification of hybrids via flow cytometry, RAPD, and secondary metabolite profiles

Banjanac¹,

Šiler²,

Škorić³

et al. 2014

Turk J Bot

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“…Nuclear rDNA repeats, mostly internal transcribed spacer (ITS) sequences, have been used to identify multiple genome donors (Blattner 2004;Guggisberg et al 2006). However, as a result of homogenization following polyploidization, ITS sequences sometimes impede the accuracy of phylogeny results (Alvarez and Wendel 2003).…”

Section: Introductionmentioning

confidence: 99%

Molecular phylogeny and reticulate origins of several American polyploid Hordeum species

WangHuan

SunGenlou

2011

Botany

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The phylogeny of diploid species in the genus Hordeum has been studied intensively. Although the origin of American polyploid species has been analyzed using multiple-copy internal transcribed spacer sequences, the origins of these species in Hordeum remain unclear. The objectives of our study were to elucidate the origins of American polyploid species and to explore phylogenetic relationships of these polyploids to diploid Hordeum and other diploid species in Triticeae using a single copy of a nuclear gene, disrupted meiotic cDNA1 (DMC1). DMC1 sequences from nine Hordeum polyploid species were analyzed. Sequence comparisons revealed that one copy of sequences from polyploid species Hordeum fuegianum, Hordeum jubatum, and Hordeum tetraploidum showed a 82 bp miniature inverted-repeat terminal element (MITE) (Stowaway) insertion, which was also detected in the Triticeae diploid species Australopyrum species (W genome) and Taeniatherum caput-medusae (Ta genome). Maximum parsimony and Bayesian analysis suggested that diploid Hordeum brachyantherum subsp. californicum is one ancestor of polyploids Hordeum arizonicum, H. brachyantherum subsp. brachyantherum, Hordeum depressum, and Hordeum procerum. The other ancestor of tetraploid H. depressum is probably Hordeum euclaston. Hordeum cordobense was suggested to be one of the genome donors to hexaploid H. procerum. The diploid Hordeum flexuosum and tetraploid H. tetraploidum were suggested as the parents to hexaploid species Hordeum parodii. The result is that one sequence from each of three Hordeum tetrapolyploids, including H. fuegianum, H. jubatum, and H. tetraploidum, and one from Hordeum hexaploid H. arizonicum fall outside the Hordeum clade of the DMC1 phylogenetic tree, therefore representing another example of complex evolutionary history. Our data may shed light on future phylogenetic studies in Triticeae, especially for the polyploids, by broadening the scope of investigations through sampling more genome types in Poaceae, not only from the tribe Triticeae. Résumé : La phylogénie d'espèces diploïdes, au sein du genre Hordeum, a été étudiée intensivement. Bien que l'origine des espèces polyploïdes d'Amérique ait été analysée en utilisant les séquences de l'espaceur interne transcrit en copies multiples, les origines des espèces d' Hordeum demeurent floues. Cette étude a pour objectifs d'élucider les origines d'espèces polyploïdes d'Amérique et d'explorer les relations phylogénétiques entre ces polyploïdes les Hordeum diploïdes ainsi que d'autres espèces diploïdes chez les Triticeae, en utilisant un gène nucléaire en simple copie, le cADN1 méiotique perturbé (C1MP). Les séquences C1MP provenant de neuf espèces d'Hordeum polyploïdes ont été analysées. Les comparaisons de séquence ont révélé qu'une copie des séquences provenant des espèces polyploïdes H. fuegianum, H. jubatum, et H. tetraploidum montrait l'insertion d'un élément terminal inversé-répété miniature (ETMI) (stowaway) de 82 pb, lequel a aussi été détecté chez les espèces de Triticeae diploïdes Australopy...

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“…Whilst the nucleotide sequences of the genic fractions of both groups of rDNA genes are highly conserved throughout the plant kingdom, the intergenic spacers (IGS) in the two groups of rRNA genes and the internal transcribed spacers (ITS) of the 18S-26S rDNA display significant divergence among closely related species. IGS and ITS sequences can, therefore, be used for identifying the progenitor species in allopolyploids (Widmer and Baltisberger 1999;Guggisberg et al 2006). Physical mapping of 5S and 18S-26S rRNA genes on metaphase chromosomes and interphase nuclei in allopolyploids and their putative parents using fluorescence in situ hybridisation (FISH) enables study of the distribution, organisation and evolution of these genes (Lim et al 2000;Seijo et al 2004;Weiss-Schneeweiss et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Molecular and cytogenetic evidence for an allotetraploid origin of Trifolium dubium (Leguminosae)

2007

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Suckling clover, Trifolium dubium Sibth., is a European grassland legume that has spread to many parts of the world. The present work shows that it is an allotetraploid (2n = 4x = 30) combining the genomes of T. campestre Schreb. (2n = 2x = 14) and T. micranthum Viv. (2n = 2x = 16), two diploid species of similar geographic distribution. T. dubium has two nuclear ITS sequences that closely match those of T. campestre and T. micranthum. Genomic in situ hybridisation using genomic DNA of T. campestre and T. micranthum as probes has differentiated the ancestral sets of chromosomes in T. dubium cells. Comparative fluorescence in situ hybridisation analyses of 5S and 18S-26S rDNA loci were also consistent with an allotetraploid structure of the T. dubium genome. A marked preponderance of ITS repeats from T. campestre over those from T. micranthum indicated that concerted evolution has resulted in partial homogenisation of these sequences by depletion of the T. micranthum-derived 18S-26S rDNA repeats. In parallel with this, the epigenetic phenomenon of nucleolar dominance has been observed in T. dubium such that the chromatin associated with the 18S-26S rDNA loci derived from T. campestre is decondensed (transcriptionally active), whilst that from T. micranthum remains highly condensed throughout the cell cycle. T. dubium, therefore, appears to have arisen by way of hybridisation between forms of the diploid species T. campestre and T. micranthum accompanied by chromosome doubling. The observed genomic changes in rDNA resulting from interspecific hybridisation provide evidence for the process of genome diploidisation in T. dubium.

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Allopolyploid Origin of the Mediterranean Endemic, <I>Centaurium bianoris</I> (Gentianaceae), Inferred by Molecular Markers

Cited by 35 publications

References 47 publications

Interspecific in vitro hybridization in genus Centaurium and identification of hybrids via flow cytometry, RAPD, and secondary metabolite profiles

Interspecific in vitro hybridization in genus Centaurium and identification of hybrids via flow cytometry, RAPD, and secondary metabolite profiles

Molecular phylogeny and reticulate origins of several American polyploid Hordeum species

Molecular and cytogenetic evidence for an allotetraploid origin of Trifolium dubium (Leguminosae)

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