Allogenous skin fibroblast transplantation enhances excisional wound healing following alloxan diabetes in sheep, a randomized controlled trial

Kazemi‐Darabadi, Siamak; Sarrafzadeh‐Rezaei, Farshid; Farshid, Amir Abbas; Dalir‐Naghadeh, Bahram

doi:10.1016/j.ijsu.2014.06.007

Cited by 21 publications

(10 citation statements)

References 35 publications

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“…To apply the growth factors, there are several procedures combined with surgical technique. Transplantation of skin fibroblasts into diabetic sheep with excisional wounds significantly increased the number of blood vessels and accelerated wound closure [145]. Cultured allogenic keratinocytes contributed for patients with venous ulcers or extensive burns with regard to clinical benefits [146, 147].…”

Section: Reviewmentioning

confidence: 99%

Advances in surgical applications of growth factors for wound healing

2019

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Growth factors have recently gained clinical importance for wound management. Application of recombinant growth factors has been shown to mimic cell migration, proliferation, and differentiation in vivo, allowing for external modulation of the healing process. Perioperative drug delivery systems can enhance the biological activity of these growth factors, which have a very short in vivo half-life after topical administration. Although the basic mechanisms of these growth factors are well understood, most have yet to demonstrate a significant impact in animal studies or small-sized clinical trials. In this review, we emphasized currently approved growth factor therapies, including a sustained release system for growth factors, emerging therapies, and future research possibilities combined with surgical procedures. Approaches seeking to understand wound healing at a systemic level are currently ongoing. However, further research and consideration in surgery will be needed to provide definitive confirmation of the efficacy of growth factor therapies for intractable wounds.

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Section: Reviewmentioning

confidence: 99%

Advances in surgical applications of growth factors for wound healing

2019

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“…A retrospective study by Auxenfans et al [178] showed that cultured allogenic keratinocytes allowed fast healing of deep second degree burns in human patients. Kazemi-Darabadi et al [179] described that allogenous skin fibroblast transplantation for diabetic wounds in sheep positively affected the wound healing by increasing re-epithelialization, number of fibroblasts and blood vessels.…”

Section: Regenerative Therapies For Skin Wound Managementmentioning

confidence: 99%

Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

Borena

Martens

Broeckx

et al. 2015

Cell Physiol Biochem

168

112

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Mammal skin has a crucial function in several life-preserving processes such as hydration, protection against chemicals and pathogens, initialization of vitamin D synthesis, excretion and heat regulation. Severe damage of the skin may therefore be life-threatening. Skin wound repair is a multiphased, yet well-orchestrated process including the interaction of various cell types, growth factors and cytokines aiming at closure of the skin and preferably resulting in tissue repair. Regardless various therapeutic modalities targeting at enhancing wound healing, the development of novel approaches for this pathology remains a clinical challenge. The time-consuming conservative wound management is mainly restricted to wound repair rather than restitution of the tissue integrity (the so-called “restitutio ad integrum”). Therefore, there is a continued search towards more efficacious wound therapies to reduce health care burden, provide patients with long-term relief and ultimately scarless wound healing. Recent in vivo and in vitro studies on the use of skin wound regenerative therapies provide encouraging results, but more protracted studies will have to determine whether the effect of observed effects are clinically significant and whether regeneration rather than repair can be achieved. For all the aforementioned reasons, this article reviews the emerging field of regenerative skin wound healing in mammals with particular emphasis on growth factor- and stem cell-based therapies.

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“…Keratinocytes are critical in reepithelization for proper closure of the wound surface, and fibroblasts actively remodel matrix proteins to fill the damaged dermal tissue 36 . Thanks to their importance, these two cell types are most widely utilized for cell-based skin regeneration therapy 37–42 . During normal wound healing, fibroblasts transiently exhibit the activated phenotype of myofibroblasts which feature higher cellular contractility and produce excessive matrix proteins to promote the faster closure of the wound 43 .…”

Section: Introductionmentioning

confidence: 99%

Physicochemically Tuned Myofibroblasts for Wound Healing Strategy

Choi

Choung

et al. 2019

Sci Rep

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Normal healing of skin wounds involves a complex interplay between many different cellular constituents, including keratinocytes, immune cells, fibroblasts, myofibroblasts, as well as extracellular matrices. Especially, fibroblasts play a critical role in regulating the immune response and matrix reconstruction by secreting many cytokines and matrix proteins. Myofibroblasts, which are differentiated form of fibroblasts, feature high cellular contractility and encourage the synthesis of matrix proteins to promote faster closure of the wounds. We focus on the functional characteristics of these myofibroblasts as the healing strategy for severe wounds where the surplus amount of matrix proteins could be beneficial for better regeneration. In this study, we first employed multiple physicochemical cues, namely topographical alignment, TGF-β1, and electrical field (EF), to induce differentiation of dermal fibroblasts into myofibroblasts, and to further activate the differentiated cells. We then used these cells in a mouse wound model to verify their potential as a transplantable substitute for the severe wound. Our results confirmed that physicochemically stimulated myofibroblasts promoted faster healing of the wound compared to the case with non-stimulated myofibroblasts through elevated matrix reconstruction in the mouse model. Conclusively, we propose the utilization of physicochemically tuned myofibroblasts as a novel strategy for promoting better healing of moderate to severe wounds.

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Allogenous skin fibroblast transplantation enhances excisional wound healing following alloxan diabetes in sheep, a randomized controlled trial

Cited by 21 publications

References 35 publications

Advances in surgical applications of growth factors for wound healing

Advances in surgical applications of growth factors for wound healing

Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

Physicochemically Tuned Myofibroblasts for Wound Healing Strategy

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