Allogenic Serum Improves Cold Preservation of Osteochondral Allografts

Abstract

Background Although several types of culture medium have been used for preservation of osteochondral allografts, the viability of chondrocytes decreases with increasing storage duration. We previously showed the University of Wisconsin solution is more suitable for graft preservation than culture medium. Questions/purposes We determined whether the addition of allogenic serum to University of Wisconsin solution increases chondrocyte survival during prolonged storage of osteochondral allografts. Methods Osteochondral tissue samples harvested from the distal femora of rats were preserved in University of Wisconsin solution supplemented with 0%, 1%, 10%, and 50% allogenic serum at 4°C for 14 days. Cell viability and chondrocyte degenerative changes of the samples then were assessed using a tetrazolium assay and histologic methods. We also evaluated time-dependent changes in cell viability and histologic findings of samples preserved for 7, 14, and 21 days in University of Wisconsin solution supplemented with or without 10% allogenic serum. Results After 14 days of preservation, osteochondral tissue samples maintained in University of Wisconsin solution containing 10% or greater allogenic serum exhibited the highest cell viability and lowest degenerative changes in chondrocytes. In the evaluation of time-dependent changes, we found the chondrocyte degenerative changes were greater in cartilage preserved in University of Wisconsin solution alone than in University of Wisconsin solution containing 10% allogenic serum after Day 7 or later. Conclusions Our results suggest the addition of 10% allogenic serum to University of Wisconsin solution enhances viability of osteochondral tissue samples. Clinical Relevance The use of allogenic serum-supplemented University of Wisconsin solution is expected to prolong the duration of osteochondral allograft storage and result in higher-quality grafts.

Acceleration of periosteal bone formation by human basic fibroblast growth factor containing a collagen‐binding domain from Clostridium histolyticum collagenase

“…To date, several growth factors have been modified with CBDs from von Willebrand factor (vWF), mammalian collagenase, and fibronectin, with the recombinant proteins displaying increased collagen‐binding activity without impacting cytokine activity. Here, the CBD derived from C. histolyticum collagenase did not modify the in vitro biological activity of bFGF, as shown by its capacity to promote periosteal mesenchymal cell proliferation.…”

Protocolo para captação, transporte e preservação de tecido osteocondral humano

“…Structural changes in the superficial layer allow the flow of synovial fluid, leading to the formation of subchondral cysts, thus initiating the process of joint degeneration. 21 Our work had some limitations. First, there were no quantitative analyses of cell viability of the tissue studied.…”

“…Several supplemented media were evaluated for their benefits on the viability of preserved cartilaginous tissue, including dexamethasone-associated media, 19 hyaluronic acid, 20 allogeneic serum, 21 or tumor necrosis factor inhibitor α 19 and the cellular apoptosis inhibiting agent, 22 among others. The divergence in the literature between the means used, the absence of in vivo research, and the high cost of the preservation methods evaluated, make it difficult to decide on the best supplement to use in conjunction with preservation media to improve the quality of tissue stored for transplantation.…”

Approaches to preserve human osteochondral allografts

“…Teng et al (2008) showed that adding recombinant IGF-1 and an apoptosis inhibitor called ZVAD-fmk to DMEM improved chondrocyte survival in bovine osteochondral grafts. Onuma et al (2012) observed that supplementation of University of Wisconsin solution with allogeneic serum enhanced the viability of rat osteochondral samples for 14 days. Pearsall et al (2004) had also indicated that osteochondral allografts could be refrigerated in a culture medium in temperatures ranging from 2 to 8°C maintaining 67 % of chondrocyte viability for up to 44 days prior to implantation.…”