Allogenic dendritic cell and tumor cell fused vaccine for targeted imaging and enhanced immunotherapeutic efficacy of gastric cancer

Li, Chao; Liang, Shujing; Zhang, Chunlei; Liu, Yanlei; Yang, Meng; Zhang, Jingpu; Zhi, Xiao; Pan, Fei; Cui, Daxiang

doi:10.1016/j.biomaterials.2015.03.024

Cited by 54 publications

(45 citation statements)

References 42 publications

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“…Li et al reported an allogenic DC and tumor cell fused vaccine combined with cytokine-induced killing cells (CIKs) for targeted imaging and enhanced immunotherapeutic efficacy of gastric cancer (GC). 735 The fused vaccine was prepared by PEG-mediated fusion between mature DCs and inactive GC MGC803 cells. The immunotherapeutic and prophylactic potentials of the fused cells (FCs) were evaluated in tumor-bearing, postsurgery, and tumor-free mice models.…”

Section: In Vivo Combination Of Diagnosis and Treatment (“Theranostics”)mentioning

confidence: 99%

Diverse Applications of Nanomedicine

Pelaz¹,

et al. 2017

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The design and use of materials in the nanoscale size range for addressing medical and health-related issues continues to receive increasing interest. Research in nanomedicine spans a multitude of areas, including drug delivery, vaccine development, antibacterial, diagnosis and imaging tools, wearable devices, implants, high-throughput screening platforms, etc. using biological, nonbiological, biomimetic, or hybrid materials. Many of these developments are starting to be translated into viable clinical products. Here, we provide an overview of recent developments in nanomedicine and highlight the current challenges and upcoming opportunities for the field and translation to the clinic.

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Section: In Vivo Combination Of Diagnosis and Treatment (“Theranostics”)mentioning

confidence: 99%

Diverse Applications of Nanomedicine

Pelaz¹,

et al. 2017

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“…4, prepared EGFR-Au@AgNR nanotags did not exhibit the obvious cytotoxicity to MGC 803 cells within the scope of 2 nM [44][45][46]. with PBS for 3 times, and then SERS measurements were done as shown in Fig.…”

Section: Cytotoxicity and Targeting Ability Of Egfrau@ag Nr Nanotagsmentioning

confidence: 99%

EGFR Antibody Conjugated Bimetallic Au@Ag Nanorods for Enhanced SERS-Based Tumor Boundary Identification, Targeted Photoacoustic Imaging and Photothermal Therapy

Chen¹,

Zhang²,

Yang³

et al. 2016

Nano BioMed ENG

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The development of multifunctional nanoprobes for simultaneous targeted imaging, tumor boundary identification and photothermal therapy of gastric cancer has become a great challenge. Herein, EGFR monoclonal antibody-conjugated Au@Ag nanorods decorated with DTNB nanoprobes (5,5'-Dithiobis-(2-nitrobenzoic acid)) (EGFR-Au@Ag NR nanotags) were prepared and characterized. Their biocompatibility was analyzed by MTT assay. In vitro studies show that EGFR-Au@Ag NR nanotags own good biocompatibility and capability of targeting and entering into gastric cancer MGC803 cells, silver nano-film layer on the surface of gold nanorods remarkably enhance surfaceenhanced rama spectra (SERS) signal, enhanced photoacoustic imaging efficacy and photothermal conversion efficiency of gold nanorods. In vivo studies show that prepared nanotags could target actively gastric cancer cells at 2 h post-injection, and distributed in the tumor site, exhibited enhanced SERS signals to display clearly tumor boundary, enhanced photoacoustic imaging to display clearly tumor boundary. Under 1 w/cm 2 laser irradiation, tumor growth was remarkably inhibited by local photothermal therapy. In conclusion, high performance EGFR-antibody conjugated Au@Ag nanorod-DTNB nanoprobes exhibit great potential in applications such as targeted photoacoustic imaging, simultaneous tumor boundary identification and selective photothermal therapy of gastric cancer in the near future.

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“…PNV decoration of CSCs was performed by mixing cells in the presence of polyethylene glycol (PEG), which is widely used to aid cell fusion 28–31 . Briefly, 1 × 10 6 DiI-labelled CSCs and 1 × 10 10 DiO-labelled PNVs were mixed in 50 μl PEG for 5 mins.…”

Section: Methodsmentioning

confidence: 99%

Targeted repair of heart injury by stem cells fused with platelet nanovesicles

et al. 2018

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Stem cell transplantation, as used clinically, suffers from low retention and engraftment of the transplanted cells. Inspired by the ability of platelets to recruit stem cells to sites of injury on blood vessels, we hypothesized that platelets might enhance the vascular delivery of cardiac stem cells (CSCs) to sites of myocardial infarction injury. Here, we show that CSCs with platelet nanovesicles fused onto their surface membranes express platelet surface markers that are associated with platelet adhesion to injury sites. We also find that the modified CSCs selectively bind collagen-coated surfaces and endothelium-denuded rat aortas, and that in rat and porcine models of acute myocardial infarction the modified CSCs increase retention in the heart and reduce infarct size. Platelet-nanovesicle-fused CSCs thus possess the natural targeting and repairing ability of their parental cell types. This stem cell manipulation approach is fast, straightforward and safe, does not require genetic alteration of the cells, and should be generalizable to multiple cell types.

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Allogenic dendritic cell and tumor cell fused vaccine for targeted imaging and enhanced immunotherapeutic efficacy of gastric cancer

Cited by 54 publications

References 42 publications

Diverse Applications of Nanomedicine

Diverse Applications of Nanomedicine

EGFR Antibody Conjugated Bimetallic Au@Ag Nanorods for Enhanced SERS-Based Tumor Boundary Identification, Targeted Photoacoustic Imaging and Photothermal Therapy

Targeted repair of heart injury by stem cells fused with platelet nanovesicles

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