Allogeneic Serum Eye Drops for the Treatment of Dry Eye Patients with Chronic Graft-Versus-Host Disease

Na, Kyung‐Sun; Kim, Man Soo

doi:10.1089/jop.2012.0002

Cited by 79 publications

(58 citation statements)

References 30 publications

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“…Alongside a number of other tests (including OSDI, Schirmer, tear break-up time [TBUT], tear osmolarity and cornea staining score), IC was performed to measure grade and goblet cell density pre-and postautologous serum therapy (4 weeks). This group reported that, as well as improvement in the other tests, improved IC grade and goblet cells density improved from 3.4 to 2.4, and from 90.6 to 122.6 cell/mm 2 , respectively [41]. A similar study, albeit without autologous serum therapy, also used IC on 40 GvHD subjects with and without ocular involvement and they noted DED in 30 of the subjects and abnormal cell morphologies in 75% of all GvHD IC samples [42].…”

Section: Ocular Graft-versus-host Diseasementioning

confidence: 90%

“…Recently, IC has also been used to assess morphological changes and novel biomarkers in subjects with oGvHD. For example, Na et al [41] investigated subjects with chronic GvHD following allogeneic hematological stem cell transplantation that had been diagnosed with refractory DED. Alongside a number of other tests (including OSDI, Schirmer, tear break-up time [TBUT], tear osmolarity and cornea staining score), IC was performed to measure grade and goblet cell density pre-and postautologous serum therapy (4 weeks).…”

Section: Ocular Graft-versus-host Diseasementioning

confidence: 99%

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Biomarkers of Ocular Surface Disease Using Impression Cytology

Hagan

2017

Biomark. Med.

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Impression cytology (IC) is a technique which permits the retrieval of the outermost layer of ocular surface cells via the use of various types of filters. It is a minimally invasive method of evaluating human conjunctival epithelial cell morphology in the diagnosis of dry eye disease, a common and distressing disorder associated with ageing, contact lens wear, autoimmune disorders and refractive (LASIK) surgery. IC may also be utilized in the diagnosis of other ocular diseases, such as keratoconus and thyroid orbitopathy. More recently, IC has been utilized for the subsequent investigation of gene and protein expression of conjunctival cells in order to identify novel diagnostic biomarkers and to further our understanding of the mechanisms underlying ocular surface disease. This review will therefore examine the literature concerning the role of IC in identifying cellular markers of eye disease, systemic diseases with ocular involvement and potential novel therapeutic targets. Impression cytology (IC) is a minimally invasive method of evaluating human ocular surface cells and is considered as the 'gold standard' technique for assessing cell morphology. Introduced by Thatcher et al. [1] and Egbert et al. [2], IC is an easy-to-use technique of directly retrieving (mainly conjunctival) cells, using various filter types which are gently applied to exposed (interpalpebral) conjunctival surfaces. The filters used include cellulose acetate [3], polycarbonate (Biopore, Millipore UK Ltd, Watford, UK) [4], nitrocellulose [5] or polyethersulfone (PES) [6] which are gently applied to exposed (interpalpebral) conjunctival surfaces. Recently, a more standardized tool for IC sampling has been introduced (Figures 1 & 2). The Eyeprim is a new single-use PES filter sampling medical device for retrieval of conjunctival cells (Opia Technologies, France) and a recent study by Lopez-Miguel et al. [7] performed an analysis of this tool versus the traditional IC method (PES). On a cohort of 20 healthy individuals, they extracted and compared RNA from both devices and found no significant differences in the mean yield (p ≥ 0.45), the total RNA amount (μg), or the amount normalized to membrane area [7]. Although an earlier study of the Eyeprim [8], suggested that it recovered more cells than traditional IC, Lopez-Miguel et al. [7] did not observe differences in the RNA collected between this device and PES.Historically, IC has been used to analyze pathological changes to conjunctival cells for indicators of squamous metaplasia in ocular surface disease (OSD) [9]. More recently, however, researchers have used IC to examine specific markers of inflammation, for example, HLA-DR, ICAM-1 and CD8 [10][11][12], and to examine novel biomarkers of dry eye disease (DED). The latter is performed in an attempt to understand the mechanisms underpinning DED pathophysiology and is now feasible, due to vast improvements in the assay sensitivities for gene and protein examination [13]. As a result, IC membranes can be subsequently analyzed using techniq...

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Section: Ocular Graft-versus-host Diseasementioning

confidence: 90%

Section: Ocular Graft-versus-host Diseasementioning

confidence: 99%

Biomarkers of Ocular Surface Disease Using Impression Cytology

Hagan

2017

Biomark. Med.

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“…Serum contains growth factors and vitamins such as epithelial growth factor, platelet derived growth factor, vitamin A and E, fibronectin, and albumin, which are required for corneal epithelial proliferation and repair and often reduced in dry eye syndrome [5][6][7][8][9]. Many studies have reported that both allogeneic and autologous serum eye drops are effective in alleviating the symptoms of dry eye in different patient cohorts [10][11][12][13][14][15][16][17][18][19], although further randomised controlled trials are warranted due to the limited number of trials and the small number of patients in the studies conducted to date [20]. Others have reported alternative therapies such as albumin [21,22] and eye drops prepared from platelet lysate [23] and platelet rich plasma [24,25] to be effective in treating severe dry eye, as these also contain many growth factors required for epithelial repair and healing [26].…”

Section: Background and History Of Eye Drop Production In Australiamentioning

confidence: 99%

“…As such allogeneic serum eye drops may be a cost-effective alternative, and they have been shown to be safe and clinically effective [13,17,19]. The New Zealand Blood Service has provided allogeneic serum eye drops since 2007, which are produced from both whole blood collected from both male and female AB donors, and account for 39% of their serum eye drop treatments [28,29].…”

Section: Future Considerationsmentioning

confidence: 99%

Serum eye drop preparation in Australia: Current manufacturing practice

Marks¹,

Fisher²,

Mondy³

et al. 2015

Transfusion and Apheresis Science

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“…Pharmacological approaches comprise above all hydration of the ocular surface by artificial tears; anti-inflammatory and immunosuppressory agents are used to improve the symptoms of chronic inflammation 21,22 . It has been shown that autologous serum (AS) eye drops can be profitably used in the treatment of DED of various etiologies, including GvHD and Sjögren's syndrome [23][24][25][26] . Their effectiveness is mainly attributed to the presence of various substances including growth factors such as TGFβ, epidermal growth factor (EGF), nerve growth factor and insulin-like growth factor 1; neurotrophic factors (substance P); bacteriostatic factors (lysozyme, immunoglobulins); fibronectin and vitamin A.…”

Section: Introductionmentioning

confidence: 99%