Alloferon-1 ameliorates acute inflammatory responses in λ-carrageenan-induced paw edema in mice

Zhang, Xiangrui; Retyunskiy, Vladimir; Qiao, Sanzheng; Zhao, Yupei; Tzeng, Chi-Meng

doi:10.1038/s41598-022-20648-z

Cited by 14 publications

(10 citation statements)

References 43 publications

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“…In brief, compounds 8 and 9 were capable of decreasing paw edema, which was verified by a prominent percentage of inhibition, such as 44.83% and 49.29% respectively, at the end of the experiment. Understanding the mechanisms of acute inflammatory response is the basic step in proposing novel compounds as anti-inflammatory agents [ 45 ]. As relevant and widely used for testing novel compounds, carrageenan-induced paw edema represents an acute inflammation model characterized by biphasic edema.…”

Section: Resultsmentioning

confidence: 99%

“…As relevant and widely used for testing novel compounds, carrageenan-induced paw edema represents an acute inflammation model characterized by biphasic edema. During the first hour, there is a release of histamine, serotonin, tachykinin, bradykinin, and other pro-inflammatory agents that immediately cause clinical manifestations such as edema, hyperalgesia, and erythema, appearing immediately [ 45 , 46 ]. This is in accordance with our observations, i.e., the greatest paw edema because of venous obstruction and elevated vascular permeability was noticed in all groups in the first two hours [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

“…basic step in proposing novel compounds as anti-inflammatory agents [45]. As relevant and widely used for testing novel compounds, carrageenan-induced paw edema represents an acute inflammation model characterized by biphasic edema.…”

Section: Investigation Of Cox-2 and 5-lox Enzyme Inhibitory Propertiesmentioning

confidence: 99%

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Synthesis, Characterization, and Investigation of Anti-Inflammatory and Cytotoxic Activities of Novel Thiourea Derivatives of Naproxen

Nedeljković,

Nikolić,

Čanović

et al. 2023

Pharmaceutics

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The objective of this study was to synthesize seven novel thiourea derivatives of naproxen (8–14), examine the anti-inflammatory activity of the newly synthesized compounds, investigate the cytotoxic potential of both sets of synthesized compounds (1–7 and 8–14), and select the most promising anti-inflammatory and antitumor drug candidates. The results of the in vivo anti-inflammatory study clearly showed that compounds 8 and 9 were capable of decreasing paw edema, as evident from a high percentage of inhibition (44.83% and 49.29%, respectively). In addition, the results of in vitro enzyme inhibition assays demonstrated that neither of the newly synthesized compounds reached 50% inhibition of 5-LOX at concentrations lower than 100 µM. In terms of antitumor potential, derivatives 3 and 8 exhibited strong cytotoxic effects on the HeLa cell line, suggesting the involvement of the extrinsic pathway of apoptosis. According to the overall results obtained for both sets of synthesized molecules, derivatives 4 and 8 can be underlined as molecules with the strongest anti-inflammatory activity, while derivatives 3 and 8 are the most promising cytotoxic agents.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Synthesis, Characterization, and Investigation of Anti-Inflammatory and Cytotoxic Activities of Novel Thiourea Derivatives of Naproxen

Nedeljković,

Nikolić,

Čanović

et al. 2023

Pharmaceutics

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“…Additionally, in HR-1 hairless mice, topical application of alloferon prevented epithelial thickness induced by UVB irradiation, indicating an inhibitory effect on IL-6 production 119. Furthermore, alloferon has been shown to attenuate acute inflammatory response in λ-carrageenan-induced mouse paw edema in our previous study (Zhang et al, 2022). Administration of the peptide, suppressed the expression of pro-inflammatory cytokines such as TNF-α in inflamed paw tissue, monocyte chemoattractant protein 1 (MCP1), and IL-5 (Zhang et al, 2022).…”

Section: Anti-inflammatory Activity Of Alloferonmentioning

confidence: 87%

“…The identified amino acid sequences for alloferon are as follows: H-His-Gly-Val-Ser-Gly-His-Gly-Gln-His-Gly-Val-His-Gly-OH (alloferon I) and H-Gly-Val-Ser-Gly-His-Gly-Gln-His-Gly-Val-His-Gly-OH (alloferon II) (Chernysh et al, 2002). Alloferon has displayed anti-inflammatory and antibacterial effects, as well as immunoregulatory and immunomodulatory activities against tumors and viruses within the span of extensive research (Zhang et al, 2022). In this study, we aim to provide a comprehensive review of the biological effects and applications of alloferon and its analogues.…”

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confidence: 99%

Study of alloferon, a novel immunomodulatory antimicrobial peptide (AMP), and its analogues

Appiah,

Chen,

Pori

et al. 2024

Front. Pharmacol.

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Antimicrobial peptides (AMPs) are widely distributed throughout the biosphere and represent a class of conserved peptide molecules with intrinsic antimicrobial properties. Their broad-spectrum antimicrobial activity and low risk to induce resistance have led to increased interest in AMPs as potential alternatives to traditional antibiotics. Among the AMPs, alloferon has been addressed due to its immunomodulatory properties that augment both innate and adaptive immune responses against various pathogens. Alloferon and its analogues have demonstrated pharmaceutical potential through their ability to enhance Natural Killer (NK) cell cytotoxicity and stimulate interferon (IFN) synthesis in both mouse and human models. Additionally, they have shown promise in augmenting antiviral and antitumor activities in mice. In this article, we provide a comprehensive review of the biological effects of alloferon and its analogues, incorporating our own research findings as well. These insights may contribute to a deeper understanding of the therapeutic potential of these novel AMPs.

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Grape Pomace Polyphenols Reduce Acute Inflammatory Response Induced by Carrageenan in a Murine Model

Nery‐Flores,

Castro‐López,

Martínez‐Hernández

et al. 2024

Chemistry & Biodiversity

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Grape pomace (GP), a by‐product of wine production, contains bioactive polyphenols with potential health benefits. This study investigates the anti‐inflammatory properties of a polyphenolic fraction derived from GP, obtained by ultrasound‐microwave hybrid extraction and purified using ion‐exchange chromatography. In the inflammation model, mice were divided into six groups: intact, carrageenan, indomethacin, and three GP polyphenols treatment groups. Paw edema was induced by subplantar injection of carrageenan, and the GP polyphenols were administered intraperitoneally at doses of 10, 20, and 40 mg/kg. The anti‐inflammatory effect was evaluated by measuring paw volume, and expression of inflammatory markers: cyclooxygenase‐2 (COX‐2), myeloperoxidase (MPO), and cytokines (IL‐1β and IL‐6), along with lipid peroxidation levels. The GP polyphenols significantly reduced paw edema and expression levels of COX‐2, MPO, and cytokines in a dose‐dependent manner effect, with the highest dose showing the greatest reduction. Additionally, lipid peroxidation levels were also decreased by GP polyphenols treatment at doses of 10 and 20 mg/kg. These findings suggest that ultrasound‐microwave extraction combined with amberlite purification proved to be effective in obtaining a polyphenolic‐rich fraction from GP. Thus, GP polyphenols may serve as a natural anti‐inflammatory and antioxidant agent for treating inflammation and oxidative stress‐related diseases.

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Alloferon-1 ameliorates acute inflammatory responses in λ-carrageenan-induced paw edema in mice

Cited by 14 publications

References 43 publications

Synthesis, Characterization, and Investigation of Anti-Inflammatory and Cytotoxic Activities of Novel Thiourea Derivatives of Naproxen

Synthesis, Characterization, and Investigation of Anti-Inflammatory and Cytotoxic Activities of Novel Thiourea Derivatives of Naproxen

Study of alloferon, a novel immunomodulatory antimicrobial peptide (AMP), and its analogues

Grape Pomace Polyphenols Reduce Acute Inflammatory Response Induced by Carrageenan in a Murine Model

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