Allochroic‐Graphene Oxide Linked 3D Oriented Surface Imprinting Strategy for Glycoproteins Assays

Abstract

Affordable methodologies for screening of disease-related biomarkers are vital for early clinical diagnosis, and can potentially improve the survival rate. Herein, a cost-efficient and easy-to-use approach, called allochroic-graphene oxide linked oriented surface imprinting strategy (AGO-OSI), is imparted for rapid glycoproteins screening with naked eyes. The novel detection principle herein relies on the boronate-affinity sandwich-like assembly, in which the target glycoproteins are specifically captured by the engineered 3D oriented surface imprinting magnetic microprobe, and followed by labeling with the pH-triggered allochroic-graphene oxide. Compared with the enzyme-linked immunosorbent assay, no antibody-assisted recognition or complicated enzymatic labeling is required, endowing the AGO-OSI with the merits of low-cost, labor-saving, and user-friendly. In addition, the pH-triggered allochroic-graphene oxide provides a simple, real-time, and economical signal readout, without the sophisticated instruments requirement. Finally, it is successfully applied to rapid diagnosis of α-fetoprotein (AFP) in liver cancer event with naked eyes, further manifesting the superiority of AGO-OSI. It is believed that the cost-efficient and easy-to-use strategy proposed herein can potentially be a versatile tool for point-of-care diagnosis, especially applicable in resource-constrained regions.

Functional Interface for Glycoprotein Sensing: Focusing on Biosensors

“…Zhu, Ouyang and co-workers developed a cost-efficient and easy-to-use method, named allochroic-graphene oxide linked oriented surface imprinting strategy for quick glycoprotein screening by the naked eye. 60 Two key microprobes were designed in this strategy: 3D oriented surface imprinting magnetic nanoparticles and phenylboronic-acid-decorated allochroic-graphene oxide (PBA-AGO). The former functioned as stable and economical synthetic receptors to specifically capture glycoproteins (AFP), while PBA-AGO, functionalized with phenolphthalein, was responsible for signal amplification and readout, avoiding the use of complicated enzyme labeling in conventional ELISA.…”

“…Zhu, Ouyang and co-workers developed a cost-efficient and easy-to-use method, named allochroic-graphene oxide linked oriented surface imprinting strategy for quick glycoprotein screening by the naked eye . Two key microprobes were designed in this strategy: 3D oriented surface imprinting magnetic nanoparticles and phenylboronic-acid-decorated allochroic-graphene oxide (PBA-AGO).…”

Research status and prospect of graphene materials in aviation

“…Since the first discovery of graphene, nanomaterials with two-dimensional (2D) structures have aroused widespread research interest worldwide [59][60][61][62][63][64][65][66] . Thanks to its huge surface area to volume ratio, atomic thickness, and excellent electrical conductivity or semiconductor properties, 2D structural materials also show extraordinary potential in the field of gas detection [67][68][69][70] .…”

Preparation and specific adsorption of dual antibacterial BSA surface imprinted GO‐PEI/MXene wrinkled microspheres

“…Therefore, GO is an ideal carrier for surface protein imprinted materials. There have been several researchers who applied GO to prepare surface protein imprinting materials 11–14 . In the past, our group constructed Fe 3 O 4 @rGO wrinkled microspheres from reduced graphene oxide (rGO) and Fe 3 O 4 nanoparticles.…”

“…There have been several researchers who applied GO to prepare surface protein imprinting materials. [11][12][13][14] In the past, our group constructed Fe 3 O 4 @rGO wrinkled microspheres from reduced graphene oxide (rGO) and Fe 3 O 4 nanoparticles. The microspheres were used as carriers to prepare BSA surface imprinted materials.…”