Allocation of rhodamine-loaded nanocapsules from blood circulatory system to adjacent tissues assessedin vivoby fluorescence spectroscopy

Abstract

Modern fluorescent modalities play an important role in the functional diagnostic of various physiological processes in living tissues. Utilizing the fluorescence spectroscopy approach we observe the circulation of fluorescent-labelled nanocapsules with rhodamine tetramethylrhodamine in a microcirculatory blood system. The measurements were conducted transcutaneously on the surface of healthy Wistar rat thighs in vivo. The administration of the preparation capsule suspension with a rhodamine concentration of 5 mg kg −1 of the animal weight resulted in a twofold increase of fluorescence intensity relative to the baseline level. The dissemination of nanocapsules in the adjacent tissues via the circulatory system was observed and assessed quantitatively. The approach can be used for the transdermal assessment of rhodamine-loaded capsules in vivo.

Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy

“…NCs were prepared using calcium carbonate (CaCO 3 ) particles as a sacrificial template. The CaCO 3 particles were fabricated as before [16]. CaCl 2 and Na 2 CO 3 aqueous solutions (0.33 M) were mixed under vigorous stirring for 3 h and were dissolved in 10 mL ethylene glycol, leading to precipitation of CaCO 3 particles.…”

“…4. Rhodamine-labelled NCs are NCs labeled only with Rhodamine TRITC in a shell, which is used to control the allocation of NCs [16].…”

“…Nowadays, there are numerous delivery systems such as: natural biological vehicles (such as bacteria and viruses [1,2], various cell types including red blood cells (RBCs) [3][4][5], immune cells [6,7], stem cells [8][9][10][11] and manufactured carriers (liposomes [12], micelle [13], polymeric capsules [14][15][16][17], polymeric complexes [18][19][20][21]). Polymeric nanocapsules (NCs) are a promising system of natural carriers for targeted drug delivery because of their low cytotoxicity, shell flexibility, and high in vivo stability [22][23][24][25][26].…”

Tissue mimicking phantoms for fluorescence imaging

“…Methods of fluorescence spectroscopy (FS) and fluorescence imaging (FI) demonstrate significant sensitivity to the presence of pathological changes in tissues, in particular malignant ones [2][3][4][5] . While the FS and FI approaches can be successfully used for the fluorescence contrasting at selected wavelength of the emission 6,7 , the transcutaneous fluorescence measurements of endogenous fluorophores in living tissues can be challenging due to the intersection of the emission spectra and variable absorption. Extensive studies have been conducted in this direction to develop methodologies and devices for effective distinguishing of normal and tumor tissues in various human organs (lungs, breast, colon, cervix, etc.)…”