Allocation of Cells in Mouse Blastocyst Is Not Determined by the Order of Cleavage of the First Two Blastomeres

Waksmundzka, Małgorzata; Wiśniewska, Anna; Maleszewski, Marek

doi:10.1095/biolreprod.106.053165

Cited by 17 publications

(15 citation statements)

References 29 publications

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“…If the 2PB and the frontier show no tendency to line up with Layer 1 and the equator, then such observations suggest that chance depositions have been lost: several studies have either not recorded these alignments or challenged the evidence for their existence, for example (Chroscicka et al, 2004, Motosugi et al, 2005, Waksmundzka et al, 2006, Kurotaki et al, 2007. One possibility is that significant developmental organization has been transmitted by the zygote to offset the distributions expected from our models, while another possibility is that although a chance disposition is established the biological variation in small data sets makes it difficult to find.…”

Section: Interpreting Developmental Routinesmentioning

confidence: 99%

“…and "Do they matter?" The role of early development routines has been the subject of recent intense study (Gray et al, 2004, Motosugi et al, 2005, Plusa et al, 2005, Chazaud et al, 2006, Gardner, 2006, Hiiragi et al, 2006a, Hiiragi et al, 2006b, Motosugi et al, 2006, Plusa et al, 2006, Wakmundzka et al, 2006, Zernicka-Goetz, 2006, Dietrich and Hiiragi, 2007, Gardner, 2007, Kurotaki et al, 2007, Torres-Padilla et al, 2007, Bischoff et al, 2008. In this article we consider, theoretically, how these patterns might occur during the formation of the mouse blastocyst.…”

Section: Introductionmentioning

confidence: 99%

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Theoretical exploration of blastocyst morphogenesis

Shipley¹,

Bonsall²,

Allwright³

et al. 2009

Int. J. Dev. Biol.

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A theoretical exploration of cell distribution on the mouse blastocyst is conducted. A model ball of cells represents the morula which develops into a 32-cell model blastocyst that is enclosed in a spherical surface with a hemispherical cavity at one end. In the combinatorial analysis it is assumed that each cell of the 2-cell embryo forms 16 cells in the blastocyst and that these 16 cells touch each other. The results of the analysis identify a tendency for one set of 16 cells to contribute twice as many cells to the basal solid end of the blastocyst than the other set, a developmental bias that is also found by some observers of natural blastocysts. In the geometric analysis, half the volume of the inner group of cells of the morula and blastocyst and half the volume of the surrounding shell cells, the trophectoderm, is assumed to be formed from the progeny of each 2-cell stage cell. Making various assumptions about morphogenesis, it is found that there is a tendency for a curved frontier between the volumes from each 2-cell stage cell, the clonal volumes, to lie at an angle of 43.4 o to the equator of the blastocyst and for the bulk of the frontier circumference to lie on either side of the equator. These tendencies are also found by some observers of real blastocysts.

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Section: Interpreting Developmental Routinesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Theoretical exploration of blastocyst morphogenesis

Shipley¹,

Bonsall²,

Allwright³

et al. 2009

Int. J. Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…On the other hand, others believe that the first cleavage division is somewhat random in its orientation and that there is an inherent symmetry to early mouse development (Hiiragi et al 2004;Kurotaki et al 2007;Motosugi et al 2005). Moreover, it is argued that there is no bias to the fates of the blastomeres at the 2-cell stage as determined by lineage tracing experiments (Alarcon et al 2003;Chroscicka et al 2004;Motosugi et al 2005;Waksmundzka et al 2006). Instead, blastocyst formation is inferred to be driven entirely by positional cues, due, at least in part, to the confining effects of the surrounding zona pellucida (ZP) (Dietrich et al 2007;Honda et al 2008;Kurotaki et al 2007).…”

Section: Introductionmentioning

confidence: 99%

The effect of superovulation on the contributions of individual blastomeres from 2-cell stage CF1 mouse embryos to the blastocyst

Katayama

Roberts

2010

Int. J. Dev. Biol.

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It remains controversial whether blastomeres of 2-cell stage mouse embryos show bias in their contribution to the blastocyst and whether there is any effect of superovulation. Twocell stage embryos from CF1 mice were derived by either natural breeding (N) or superovulation (S) and cultured in vitro. At blastocyst, inner cell mass and trophectoderm were distinguished by Cdx2 and Oct4 immunostaining. A fluorescent dye (CM-Dil) was also used to tag individual blastomeres at the 2-cell stage, and the descendant cells identified by their red fluorescence. S and N embryos developed to blastocyst at the same rate and contained a similar number of cells. However, with S embryos, the descendants of the blastomere labeled with CM-DiI contributed predominantly to either the embryonic or abembryonic pole about 70% of the time, whereas most N embryos displayed random patterning, with no restriction to one or other of the poles. In Sembryos, but not N-embryos, the leading blastomere at second cleavage contributed preferentially to the embryonic pole of the blastocyst and the lagging blastomere to the abembryonic pole and hence mural trophectoderm. In addition, a tetrahedral rather than a "flat" morphology at the 4-cell stage of S-embryos was strongly biased to displaying the embryonic/abembryonic pattern at blastocyst. In contrast, S-embryos lacking a zona pellucida resembled N embryos in their patterning. In CF1 mice, superovulation has little effect on development to blastocyst, but enforces a greater degree of lineage restriction than natural breeding, most likely through constraints imposed by the zona pellucida.

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“…Controversy still exists as to whether the individual blastomeres from twocell-stage embryos have identical developmental fates. However, most reports have been limited to murine species [2,7,9,15,23,29]. The distribution of active mitochondria may be indicative of the energy or ion requirements of many key events during oocyte maturation, fertilization and early embryonic development [26].…”

mentioning

confidence: 99%

The Leading Blastomere of the 2-Cell Stage Parthenogenetic Porcine Embryo Contributes to the Abembryonic Part First

et al. 2009

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ABSTRACT. Polarity formation in preimplantation embryos is controversial. To investigate the embryonic-abembryonic axis in the pig, porcine parthenotes were used to prevent the topological change caused by polyspermy as well as to avoid the influences of sperm entry position. For lineage tracing, DiI, a fluorescence dye, was injected into only a blastomere of the 2-cell stage embryos. If the first blastomere to divide was labeled, the embryo was included in the leading group, and while all others were included in the lagging group. In 60.5% of the blastocysts in the lagging group, the progeny of the labeled blastomeres formed the inner cell mass (ICM) and adjacent trophectoderm (TE) hemisphere; 62.1% of the blastocysts in the leading group had progeny of the labeled blastomeres distributed only to the TE (opposite of ICM). The rest of the lagging and leading groups showed random distributions. Unlike murine parthenotes, biased mitochondrial distribution was also found in porcine parthenotes (38.1%). Our findings indicate that the 'leading' blastomere of the 2-cell porcine parthenote forms the distal TE (abembryonic) and that the 'lagging' blastomere forms the remaining portion of the blastocyst, including the ICM (embryonic). Biased distribution of mitochondria in each 2-cell blastomere may contribute partly to this event.

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Allocation of Cells in Mouse Blastocyst Is Not Determined by the Order of Cleavage of the First Two Blastomeres

Cited by 17 publications

References 29 publications

Theoretical exploration of blastocyst morphogenesis

Theoretical exploration of blastocyst morphogenesis

The effect of superovulation on the contributions of individual blastomeres from 2-cell stage CF1 mouse embryos to the blastocyst

The Leading Blastomere of the 2-Cell Stage Parthenogenetic Porcine Embryo Contributes to the Abembryonic Part First

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