Allo-immunization elicits CD8+ T cell-derived chemokines, HIV suppressor factors and resistance to HIV infection in women

Abstract

We assessed the potential for an allogeneic-based vaccine against HIV infection in women who were allo-immunized with their partners' mononuclear leucocytes to prevent spontaneous recurrent abortion. Within 1 month of allo-immunization, there was significant upregulation in the concentrations of CD8 cell-derived suppressor factor activity, RANTES, and macrophage inflammatory proteins 1alpha and 1beta. Allo-immunization also downregulated the proportion of cells with CCR5 and CXCR4 receptors. We also found a dose-dependent decrease in HIV infectivity of CD4+ cells in vitro after allo-immunization with both primary and T-cell line adapted HIV-1. This study provides a rational basis for an alternative or complementary strategy of allo-immunization against HIV infection.

Higher macrophage inflammatory protein (MIP)-1α and MIP-1β levels from CD8+T cells are associated with asymptomatic HIV-1 infection

“…In accordance with these findings, sustained suppression of plasma HIV RNA is associated with an increase in the production of mitogen-induced MIP-1␣ and MIP-1␤ (37). Moreover, recent results indicate that human alloimmunization elicits very significant increases in the three ␤ chemokines RANTES, MIP-1␣, and MIP-1␤, and resistance of CD4 ϩ T cells to HIV infection with macrophagetropic HIV-1 strains (38). However, when the production of ␤ chemokines by unfractionated PBMCs, purified CD4 ϩ , or CD8 ϩ T cells was examined in small numbers of HIV-1 positive subjects, the analysis failed to demonstrate any association between chemokine levels and disease stage (39,40).…”

AIDS Vaccination Studies Using an Ex Vivo Feline Immunodeficiency Virus Model: Failure To Protect and Possible Enhancement of Challenge Infection by Four Cell-Based Vaccines Prepared with Autologous Lymphoblasts

“…Only one of nine animals in the mock-1 and mock-2 groups became FIV infected, a result most likely reflecting the suboptimal virus dose used. However, due to the lack of an untreated control group, we could not exclude that mock immunization contributed to prevent the full success of challenge by stimulating innate resistance or otherwise (16,58,63). In the vaccine groups the FC PLB -, ID-1-, ID-2-, and ID-3-infected animals were three, two, two, and one of five, respectively (Table 6 and Fig.…”

Myometrial cytokines and their role in the onset of labour

“…It has been suggested that trophoblasts enable appropriate tolerance by 'educating' macrophages and adapting the cytokine 231:3 profile of the local macrophages. Fest and coworkers showed that monocytes cultured with trophoblasts (Fest et al 2007) increased production of RANTES (which recruits T-regulatory cells) and MIP-1β, which both have immunosuppressive functions (Wang et al 1999, Ramhorst et al 2004. Dendritic cells (DC) promote cell tolerance particularly at the maternal-foetal interface, by priming T-regulatory (T reg ) cells (Blois et al 2007).…”