Allium hookeri root protects oxidative stress-induced inflammatory responses and β-cell damage in pancreas of streptozotocin-induced diabetic rats

Abstract

BackgroundWater extract from the root of Allium hookeri (AH) shows anti-inflammatory, antioxidant, and free radical scavenging effects. In this study, the ameliorating effects of AH on oxidative stress-induced inflammatory response and β-cell damage in the pancreas of streptozotocin (STZ)-induced type 1 diabetic rats were investigated.MethodsAH (100 mg/kg body weight/day) was orally administered every day for 2 weeks to STZ-induced diabetic rats. After the final administration of AH, biochemical parameters including glucose, insulin, reactive oxygen species levels, and protein expressions related to antioxidant defense system in the pancreas of STZ-induced diabetic rats.ResultsThe diabetic rats showed loss of body weight and increased pancreatic weight, while the oral administration of AH attenuated body and pancreatic weight changes. Moreover, the administration of AH caused a slightly decrease in the serum glucose level and a significant increase in the serum and pancreatic insulin levels in the diabetic rats. AH also significantly reduced the enhanced levels of reactive oxygen species, oxidative stress biomarker, in the serum and pancreas. The diabetic rats exhibited a down-regulation of the protein expression related to antioxidant defense system in the pancreas, but AH administration significantly up-regulated the expression of the heme oxygenase-1 (HO-1). Furthermore, AH treatment was reduced the overexpression of nuclear factor-kappa B (NF-кB)p65 and NF-кBp65-induced inflammatory cytokines such as tumor necrosis factor-α and interleukin-6. In addition, AH treatment was less pancreatic β-cell damaged compared with those of the diabetic rats.ConclusionThese results provide important evidence that AH has a HO-1 activity on the oxidative stress conditions showing pancreato-protective effects against the development of inflammation in the diabetic rats. This study provides scientific evidence that AH protects the inflammatory responses by modulated NF-кBp65 signaling pathway through activation of HO-1 in the pancreas of STZ-induced diabetic rats.

Pterostilbene Protects Cochlea from Ototoxicity in Streptozotocin-Induced Diabetic Rats by Inhibiting the Apoptosis

“…In our study, serum insulin levels were significantly lower in the diabetic rats compared to the healthy Control group and the treatment groups, likely due to the destruction of pancreatic beta-cells in the STZ-induced diabetic rats. 47,50 In accordance with the literature, PTS treatment increased serum insulin levels in a dose-dependent manner. PTS may increase insulin sensitivity and increase hepatic hexokinase activity in glycolysis.…”

“…The animal model for STZ-induced DM is characterized by elevated blood sugar levels, decreased serum insulin levels, and increased weight loss. 47 Our study showed increased weight loss in untreated diabetic rats and decreased weight loss in the diabetic rats that received PTS treatment. Compared to the other doses, 40 mg/kg/day of PTS was associated with the least amount of weight loss.…”

The new therapeutic herbal drug HM0601 and its bioactive compound rutin exert potent antiproliferative activities in mast cells

“…Allium hookeri has been consumed as medicinal food and contains a lot of rutin as an active ingredient. It is known to various biological properties such as antioxidant, antiobesity, antimicrobial, anti-inflammatory, and antiallergic effects [7][8][9][10][11][12]. Rutin, a quercetin-3-rhamnosylglucoside, has anti-inflammatory, antitumor, antiallergic, and vasoactive properties [13,14].…”

Effect of Stem Cells and Gene Transfected Stem Cells Therapy on the Pancreas of Experimentally Induced Type 1 Diabetes

“…Type 1 diabetes (T1DM), the chronic autoimmune disease, results from the destruction of the in-sulin producing beta (β) cells of the pancreas, leading to a gradual decrease in β-cell mass. Accordingly, this type of diabetes shows absolute insulin deficiency and excessive glucose production (3).…”