Allicin inhibits cell growth and induces apoptosis in U87MG human glioblastoma cells through an ERK-dependent pathway

Hun, Jae; Choi, Yoon Ji; Heon, Seung; Choi, Chang Hwa; Cho, Won Ho

doi:10.3892/or.2012.1772

Cited by 33 publications

(25 citation statements)

References 40 publications

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“…In combination, the results showed that capsaicin induced apoptosis in the MG63 cells and that the caspase cascade and antioxidant enzyme system were the underlying regulatory signaling pathways involved in the capsaicin-induced apoptosis. In a previous study, we demonstrated the effect of capsaicin on human glioblastoma U87MG cells and concluded that capsaicin induced apoptosis in the U87MG cells ( 62 ). The present results indicated that capsaicin exhibited an anticancer effect in osteosarcoma cells.…”

Section: Discussionmentioning

confidence: 96%

Capsaicin induces apoptosis in MG63 human osteosarcoma cells via the caspase cascade and the antioxidant enzyme system

Cho

Lee

Choi

et al. 2013

Molecular Medicine Reports

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Osteosarcoma is the most common malignant bone tumor in children and adolescents. This aggressive cancer mostly occurs in the long bones. Therefore, novel therapeutic approaches, such as biological therapies and gene therapy, are required to efficiently treat osteosarcoma. Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide) has been demonstrated to inhibit the growth of several types of cancer cells and a number of studies have shown that osteosarcoma may be vulnerable to biological therapies. However, little is known regarding the therapeutic effects of capsaicin on osteosarcoma. This study investigated the effects of capsaicin on MG63 human osteosarcoma cells, in addition to elucidating the regulatory signaling pathways underlying the effects of capsaicin, the caspase cascade and the antioxidant enzyme system. The MG63 cell line was treated with various concentrations of capsaicin. Cells were analyzed using MTT and flow cytometry, and the presence of DNA fragmentation was evaluated using TUNEL assay. Results showed capsaicin induced apoptosis in MG63 cells. Thus, capsaicin exhibited an anticancer effect in osteosarcoma cells.

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Section: Discussionmentioning

confidence: 96%

Capsaicin induces apoptosis in MG63 human osteosarcoma cells via the caspase cascade and the antioxidant enzyme system

Cho

Lee

Choi

et al. 2013

Molecular Medicine Reports

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“…For example, it was reported that miltefosine-induced GBM cell apoptosis is dependent on ERK1/2 activation [ 42 ]. Similarly, allicin-induced apoptosis is regulated by MAPK/ERK-dependent pathway [ 43 ]. In our hands, IL-22 treatment promotes GBM cell proliferation and protects them from apoptosis via the phosphorylation of STAT3, Akt and inactivation of ERK1/2.…”

Section: Discussionmentioning

confidence: 99%

IL22/IL-22R Pathway Induces Cell Survival in Human Glioblastoma Cells

et al. 2015

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Interleukin-22 (IL-22) is a member of the IL-10 cytokine family that binds to a heterodimeric receptor consisting of IL-22 receptor 1 (IL-22R1) and IL-10R2. IL-22R expression was initially characterized on epithelial cells, and plays an essential role in a number of inflammatory diseases. Recently, a functional receptor was detected on cancer cells such as hepatocarcinoma and lung carcinoma, but its presence was not reported in glioblastoma (GBM). Two GBM cell lines and 10 primary cell lines established from patients undergoing surgery for malignant GBM were used to investigate the expression of IL-22 and IL-22R by using quantitative RT-PCR, western blotting and confocal microscopy studies. The role of IL-22 in proliferation and survival of GBM cell lines was investigated in vitro by BrdU and ELISA cell death assays. We report herein that the two subunits of the IL-22R complex are expressed on human GBM cells. Their activation, depending on exogenous IL-22, induced antiapoptotic effect and cell proliferation. IL-22 treatment of GBM cells resulted in increased levels of phosphorylated Akt, STAT3 signaling protein and its downstream antiapoptotic protein Bcl-xL and decreased level of phosphorylated ERK1/2. In addition, IL-22R subunits were expressed in all the 10 tested primary cell lines established from GBM tumors. Our results showed that IL-22R is expressed on GBM established and primary cell lines. Depending on STAT3, ERK1/2 and PI3K/Akt pathways, IL-22 induced GBM cell survival. These data are consistent with a potential role of IL-22R in tumorigenesis of GBM. Since endogenous IL-22 was not detected in all studied GBM cells, we hypothesize that IL-22R could be activated by immune microenvironmental IL-22 producing cells.

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“…Allicin has been found to inhibit the cell viability of U87MG human glioma cells and induce cell death through apoptosis. This allicin-induced apoptosis was mediated through Bcl-2/Bax mitochondrial pathway, MAPK/ERK signalling pathway and antioxidant enzyme systems [ 28 ]. Study on the effect of allicin on the growth of human liver cancer Hep G2 cells showed that allicin induced p53-mediated autophagy and inhibited the viability of human hepatocellular carcinoma cell lines.…”

Section: Sulphur Compoundsmentioning

confidence: 99%

“…Mechanistically it was demonstrated that allicin stimulates the JNK pathway activation, which ultimately results in mitochondrial translocation of Bax and release of cytochrome C from mitochondria to cytosol, thus inducing the apoptosis of SKOV3 cells [ 65 ]. A subsequent study suggested that allicin might inhibit the cell viability and induce apoptosis in U87MG glioblastoma cells via regulating the Bac/Bcl2 and MAPK/ERK signalling pathways [ 28 ]. The other compound called ajoene, the organo-sulphur compound of garlic, has also been reported to induce apoptosis in the human promyeloleukaemic cell line (HL-60) through the generation of reactive oxygen species (ROS).…”

Section: Biological Activities Of Sulfur Compounds From mentioning

confidence: 99%

Prevailing Knowledge on the Bioavailability and Biological Activities of Sulphur Compounds from Alliums: A Potential Drug Candidate

et al. 2020

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Allium sativum (garlic) is widely known and is consumed as a natural prophylactic worldwide. It produces more than 200 identified chemical compounds, with more than 20 different kinds of sulfide compounds. The sulfide compounds particularly are proven to contribute to its various biological roles and pharmacological properties such as antimicrobial, antithrombotic, hypoglycemic, antitumour, and hypolipidemic. Therefore, it is often referred as disease-preventive food. Sulphur-containing compounds from A. sativum are derivatives of S-alkenyl-l-cysteine sulfoxides, ajoene molecules, thiosulfinates, sulfides, and S-allylcysteine. This review presents an overview of the water-soluble and oil-soluble sulphur based phytochemical compounds present in garlic, highlighting their mechanism of action in treating various health conditions. However, its role as a therapeutic agent should be extensively studied as it depends on factors such as the effective dosage and the suitable method of preparation.

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Allicin inhibits cell growth and induces apoptosis in U87MG human glioblastoma cells through an ERK-dependent pathway

Cited by 33 publications

References 40 publications

Capsaicin induces apoptosis in MG63 human osteosarcoma cells via the caspase cascade and the antioxidant enzyme system

Capsaicin induces apoptosis in MG63 human osteosarcoma cells via the caspase cascade and the antioxidant enzyme system

IL22/IL-22R Pathway Induces Cell Survival in Human Glioblastoma Cells

Prevailing Knowledge on the Bioavailability and Biological Activities of Sulphur Compounds from Alliums: A Potential Drug Candidate

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