Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers

Gao, Jingxia; Song, Guanzhong; Shen, Huan; Wu, Yiming; Zhao, Chongqi; Zhang, Zhuo; Jiang, Qian; Li, Xilong; Ma, Xiaokang; Tan, Bin; Yin, Yulong

doi:10.3389/fimmu.2022.847861

Cited by 7 publications

(8 citation statements)

References 47 publications

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“…In addition to against bacteria, allicin has also been shown to modulate the composition of gut microbiota (GM) and increase the diversity of beneficial bacteria in animal models ( Guillamón et al, 2021 ). Allicin has been found to play a role in improving intestinal epithelial barrier function and preventing barrier damage via a microbiota-regulated short-chain fatty acid-TLR4/MyD88/NF-κB cascade response in an acrylamide-induced rat model ( Yuan et al, 2021 ; Gao et al, 2022 ). Researchers suggested that allicin could block intestinal bacterial translocation by increasing the immunologic barrier function of mesenteric lymph nodes by modulating dendritic cell maturation ( Zhang Y. et al, 2017 ).…”

Section: Allicinmentioning

confidence: 99%

Allicin in Digestive System Cancer: From Biological Effects to Clinical Treatment

Yang

Luo

et al. 2022

Front. Pharmacol.

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Allicin is the main active ingredient in freshly-crushed garlic and some other allium plants, and its anticancer effect on cancers of digestive system has been confirmed in many studies. The aim of this review is to summarize epidemiological studies and in vitro and in vivo investigations on the anticancer effects of allicin and its secondary metabolites, as well as their biological functions. In epidemiological studies of esophageal cancer, liver cancer, pancreatic cancer, and biliary tract cancer, the anticancer effect of garlic has been confirmed consistently. However, the results obtained from epidemiological studies in gastric cancer and colon cancer are inconsistent. In vitro studies demonstrated that allicin and its secondary metabolites play an antitumor role by inhibiting tumor cell proliferation, inducing apoptosis, controlling tumor invasion and metastasis, decreasing angiogenesis, suppressing Helicobacter pylori, enhancing the efficacy of chemotherapeutic drugs, and reducing the damage caused by chemotherapeutic drugs. In vivo studies further demonstrate that allicin and its secondary metabolites inhibit cancers of the digestive system. This review describes the mechanisms against cancers of digestive system and therapeutic potential of allicin and its secondary metabolites.

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Section: Allicinmentioning

confidence: 99%

Allicin in Digestive System Cancer: From Biological Effects to Clinical Treatment

Yang

Luo

et al. 2022

Front. Pharmacol.

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“…Previous research has shown that LPS lowers expression of TJ proteins, which affects the intestinal barrier . LPS inhibited the IPEC-J2 cell monolayer model’s protein expression of ZO-1and claudin-1 . SC06 has the potential to reverse the LPS-induced downregulation of TJ protein expression.…”

Section: Discussionmentioning

confidence: 90%

“…42 LPS inhibited the IPEC-J2 cell monolayer model's protein expression of ZO-1and claudin-1. 43 SC06 has the potential to reverse the LPS-induced downregulation of TJ protein expression. In addition, weaned pigs treated with B. amyloliquefaciens showed an increase in the mucosal expression of TJ proteins in the ileum.…”

Section: Discussionmentioning

confidence: 99%

Bacillus amyloliquefaciens SC06 Relieving Intestinal Inflammation by Modulating Intestinal Stem Cells Proliferation and Differentiation via AhR/STAT3 Pathway in LPS-Challenged Piglets

Wang,

Zhou

et al. 2024

J. Agric. Food Chem.

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Bacillus amyloliquefaciens is a well-accepted probiotic, with many benefits for both humans and animals. The ability of intestinal stem cells (ISCs) to develop into several intestinal epithelial cell types helps accelerate intestinal epithelial regeneration. Limited knowledge exists on how bacteria regulated ISCs proliferation and regeneration. Our study investigated the effects of Bacillus amyloliquefaciens supplementation on ISC proliferation and regeneration and intestinal mucosal barrier functions in piglets exposed to lipopolysaccharide (LPS). Eighteen piglets (male, 21 days old) were randomly split into 3 clusters: CON cluster, LPS cluster, and SC06+LPS cluster. On day 21, 100 μg/kg body weight of LPS was intraperitoneally administered to the SC06+LPS and LPS groups. We found SC06 supplementation maintained the intestinal barrier integrity, enhanced intestinal antioxidant capacity, reduced generation of inflammatory response, and suppressed enterocyte apoptosis against the deleterious effects triggered by LPS. In addition, our research indicated that the SC06 supplementation not only improved the ISC regeneration, but also resulted in upregulation of aryl hydrocarbon receptor (AhR) in LPS-challenge piglets. Further studies showed that SC06 also induced ISC differentiation toward goblet cells and inhibited their differentiation to intestinal absorptive cells and enterocytes. The coculture system of SC06 and ileum organoids revealed that SC06 increased the growth of ISCs and repaired LPS-induced organoid damage through activating the AhR/STAT3 signaling pathway. These findings showed that SC06, possibly through the AhR/STAT3 pathway, accelerated ISC proliferation and promoted epithelial barrier healing, providing a potential clinical treatment for IBD. Our research demonstrated that SC06 is effective in preventing intestinal epithelial damage after pathological injury, restoring intestinal homeostasis, and maintaining intestinal epithelial regeneration.

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“…Lipopolysaccharide (LPS) is a sensitive biomarker of barrier strength and intestinal permeability [ 28 , 29 ]. LPS crosses the intestinal barrier into the bloodstream after intestinal damage and disperses throughout the body.…”

Section: Resultsmentioning

confidence: 99%

Qige Huxin Formula Attenuates Isoprenaline-Induced Cardiac Fibrosis in Mice via Modulating Gut Microbiota and Protecting Intestinal Integrity

Shi

Zuo

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. The composition and metabolic activities of gut microbiota are strongly interconnected with cardiac fibrosis (CF) and heart failure (HF). Qige Huxin formula (QHF), a traditional Chinese medicine (TCM) formulation originating from a classical Fangji Huangqi decoction, has been widely used to clinically treat HF for decades. However, it is still unclear whether QHF alleviates CF by modulating gut microbiota and intestinal integrity. Purpose. This study aimed to investigate the cardioprotective effects of QHF in isoprenaline-induced CF through modulating gut microbiota and intestinal integrity. Methods. Fifty mice were randomly divided into five groups after one week of acclimatization feeding: control group, model group, 2.56 g/kg/d group (low-dose QHF), 5.12 g/kg/d group (high-dose QHF), and meto group (15 mg/kg/d). The CF model was established by subcutaneously injecting the mice with isoprenaline (10 mg/kg/d for 14 days), followed by QHF treatment. The heart volume, cardiac weight index (CWI), serum myocardial enzymes, serum inflammatory cytokines, serum lipopolysaccharide, histopathology of the heart and colon tissues, ZO-1, and occludin of colon tissues were then measured. Fecal samples from mice were analyzed using 16S rRNA sequencing. Results. QHF treatment significantly reduced heart volume, CWI, and serum CK and CK-MB levels, attenuated cardiac histopathological alterations, and alleviated CF. QHF treatment also downregulated TNF-α, IL-1β, and IL-6 in serum. Moreover, QHF treatment decreased the serum level of lipopolysaccharide and maintained intestinal integrity by upregulating ZO-1 and occludin. The 16S rRNA microbiota analysis revealed that QHF treatment increased the relative abundance of Marvinbryantia and Phascolarctobacterium. Conclusions. QHF treatment exerts cardioprotective effects through modulating gut microbiota, protecting intestinal integrity, and alleviating inflammation. This study shows that gut microbiota may enhance heart-gut interaction.

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Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers

Cited by 7 publications

References 47 publications

Allicin in Digestive System Cancer: From Biological Effects to Clinical Treatment

Allicin in Digestive System Cancer: From Biological Effects to Clinical Treatment

Bacillus amyloliquefaciens SC06 Relieving Intestinal Inflammation by Modulating Intestinal Stem Cells Proliferation and Differentiation via AhR/STAT3 Pathway in LPS-Challenged Piglets

Qige Huxin Formula Attenuates Isoprenaline-Induced Cardiac Fibrosis in Mice via Modulating Gut Microbiota and Protecting Intestinal Integrity

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