Alleviation of Waterlogging Damage in Winter Rape by Uniconazole Application: Effects on Enzyme Activity, Lipid Peroxidation, and Membrane Integrity

Abstract

Oilseed rape (Brassica napus L.) seedlings treated with uniconazole [(E)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-l-yl)-l-penten-3-ol] were transplanted at the five-leaf stage into specially designed experimental containers and then exposed to waterlogging for 3 weeks. After waterlogging stress, uniconazole-treated seedlings had significantly higher activities of superoxide dismutase, catalase, and peroxidase enzymes and endogenous free proline content at both the seedling and flowering stages. Uniconazole plus waterlogging-treated plants had a significantly higher content of unsaturated fatty acids than the waterlogged plants. There was a parallel increase in the lipid peroxidation level and electrolyte leakage rate from the leaves of waterlogged plants. Leaves from uniconazole plus waterlogging-treated plants had a significantly lower lipid peroxidation level and electrolyte leakage rate compared with waterlogged plants at both the seedling and flowering stages. Pretreatment of seedlings with uniconazole could effectively delay stress-induced degradation of chlorophyll and reduction of root oxidizability. Uniconazole did not alter the soluble sugar content of leaves and stems, after waterlogging of seedlings. Uniconazole improved waterlogged plant performance and increased seed yield, possibly because of improved antioxidation defense mechanisms, and it retarded lipid peroxidation and membrane deterioration of plants.Key Words. Waterlogging-Uniconazole-Brassica napus L.-Enzymes-Lipid peroxidation-Membrane integrityhttp://link.springer-ny.com/link/service/journals/00344/bibs/18n1p9.html

Development of AFLP and STS markers linked to a waterlogging tolerance in Korean soybean landraces

“…In this study, we observed that the content of MDA increased in the S lines which indicated that oxidative damage in the S lines was higher than in the T lines. A rise in the MDA content was reported in susceptible species such as Brassica napus (Leul and Zhou 1999) and Iris germanica (Hunter et al 1983). In contrast, the MDA content remained the same in Iris pseudacorus, an anoxia tolerant wetland species (Hunter et al 1983).…”

“…Changes of total chlorophyll, glucose, and MDA contents during water logging treatment in the 3 tolerant (T) and 3 susceptible (S) lines. sunflower (Wample and Davies 1983) and winter rape (Leul and Zhou 1999). This means that delivery of photosynthates from leaves to roots was inhibited under flooding stress, resulting in sugar accumulation in leaves of flooded plants.…”

Consistency of Different Indices in Rapeseed (Brassica napus) may Predict the Waterlogging Tolerance

“…As to rapeseed, besides kinds of physiological and morphological indices Wang et al, 2012), waterlogging stress also altered agronomic trait to worse and finally reduces the production by 26.80% at the most (Zhu et al, 2005;Song et al, 2009). To rapeseed, one of the most important oil crops in China, its development was repressed and finally the yield was reduced with the quality characters degraded (Leul and Zhou, 1999). In our previous research, waterlogging tolerant traits of seedlings from 60 rapeseed cultivars were evaluated Li et al, 2013); one commercial rapeseed, "Chuan-you 36", was treated under waterlogging stress in different period, turning out that its yield reduced (Table 5).…”

“…Previous researches on waterlogging tolerance in rapeseed mainly focused on evaluating the effects on yields and quality characters (Wang et al, 1994;Leul and Zhou, 1999;Shi et al, 2001;Chen et al, 2006;Li et al, 2011;Wang et al, 2012;Chen et al, 2014) and could be cataloged by morphological, physiological and biochemical, as well as molecular levels. For example, (Chen et al, 2006) screened out the waterlogging tolerant rapeseed cultivars by germinating the seeds in room and treating the seedlings in fields under waterlogging; Zhang et al (2011) and Li et al (2013) evaluated the waterlogging tolerant traits of seedlings from 60 rapeseed cultivars after anoxic stress to germinated seeds by using the principle components (PCs) analysis and 2-dimensional scatter plot of the first three PC vectors, getting 18 tolerant cultivars; (Chen et al, 2014) treated a landrace "Chuan-you 36" under waterlogging stress in different period, finding that the yield reduced by 3.44% ~ 21.69% as waterlogging lasted 3~9 days.…”

The influence of Waterlogging Period on Yield and Yield Components of Mungbean (<i>Vigna radiata</i> L. Wilczek)

“…Waterlogging is a major constraint for production of many crops (Leul and Zhou, 1999). In tropical and subtropical regions, severe crop losses are caused by prolonged seasonal rainfall.…”