Alleviation of Salt Stress on Wheat (Triticum aestivum L.) by Plant Growth Promoting Bacteria strains Bacillus halotolerans MSR-H4 and Lelliottia amnigena MSR-M49

Abstract

The plant growth-promoting rhizobacteria (PGPR) application could reduce the use of synthetic fertilizers and increase the sustainability of crop production. Halophilic bacteria that have PGPR characteristics can be used in different environmental stresses. Two different strains isolated, purified, characterized as a PGPRs and phylogenetic identification using 16sRNA which was revealed to be closest matched at 99% with Bacillus halotolerans and Lelliottia amnigena. The isolates possessed plant growth promoting properties as exopolysaccharides (EPS) and indole acetic acid (IAA) production, Bacillus halotolerans had the ability to fix elemental nitrogen and the two strains have the ability to P-solubilization. Furthermore, the strains were evaluated in alleviation of different levels of salt stress on wheat plant at two experiments (Pots and a Field). Strains under study conditions significantly increased the plant height, straw dry weight (DW g plant -1 ), spike number, 1000 grain DW recorded 31.550 g with Lelliottia amnigena MSR-M49 compared to uninoculated and other strain in field, grain yield recorded 2.77 (ton fed -1 ) with Lelliottia amnigena as El-Akhdar et al.; JAMB, 20(1): 44-58, 2020; Article no.JAMB.54546 45 well as N% and protein content in grains recorded 1.213% and 6.916 respectively with inoculation with Lelliottia amnigena, also, spikes length, inoculated wheat show reduction in both proline accumulation in shoots and roots especially with Lelliottia amnigena recorded 2.79 (mg g -1 DW), inoculation significantly increased K + in root-shoot, K + /Na + in root-shoot and reduced Na + in rootshoot compared with control. This confirmed that this consortium could provide growers with a sustainable approach to reduce salt effect on wheat production. Original Research Article

Bacterial Fertilizers Based on Rhizobium laguerreae and Bacillus halotolerans Enhance Cichorium endivia L. Phenolic Compound and Mineral Contents and Plant Development

“…A similar increase in leaf mass and grain yield but in fava bean plants was also detected after the application with a biofertilizer based on a R. laguerreae strain [31]. After the application of a B. halotolerans strain, El-Akhdar and collaborators [32] reported an enhancement not only in wheat plant promotion but also in nitrogen plant content.…”

Roles of Plant Growth-Promoting Rhizobacteria (PGPR) in Stimulating Salinity Stress Defense in Plants: A Review

“…in vegetative growth stage, but rarely on evaluating the parameters that are related to reproductive stage such as grain and fruit weight, numbers of flower, numbers of seed and fruit per plant, and plant yield, etc. We found a scarcity of studies that evaluated beneficial effects of PGPB on attenuating the yield loss and on improving nutrient values [43] under saline conditions [43,104,128,[136][137][138]. In our opinion, this could be one of the main drawbacks of PGPB-related studies thus far if we consider that the improvement of crop yields, productivity, and the quality of fruit/grain under high saline conditions to be our main goal in plant agriculture studies.…”

Antimicrobial resistance determinants in silage

“…CCTCC M2018092 41 , Pantoea agglomerans 42 , Pseudomonas fulva 43 , P. putida 44 , Serratia marcescens 45 . Additional species may join the silage from animals, soil, or water: Acinetobacter gyllenbergii 46 , Citrobacter freundii 47 , Enterobacter asburiae 48 , E. cloacae 49 , E. roggenkampii 50 , E. sichuanensis 51 , Enterococcus faecalis 52 , Klebsiella aerogenes 53 , K. oxytoca 54 , Kluyvera intermedia 55 , Lactococcus garvieae 56 , Latilactobacillus curvatus 57 , Leclercia adecarboxylata 58 , Lelliottia amnigena 59 , Limosilactobacillus reuteri 28 , Providencia rettgeri 60 , Pseudescherichia vulneris 61 , Pseudomonas oryzihabitans 62 , Rhodopseudomonas palustris 63 , Serratia sp. SCBI 64 , Staphylococcus aureus 46 , S. kloosii 65 , S. pseudoxylosus 66 , S. saprophyticus 46 , Enterococcus faecium 52 .…”