Alleviation of PM2.5-induced alveolar macrophage inflammation using extract of fermented Chenopodium formosanum Koidz sprouts via regulation of NF-κB pathway

Hsieh, Chen-Che; Yu, Shu-Han; Kuo, Hsing‐Chun; Cheng, K. Y.; Hsu, Cheng-Chih; Lin, Yi‐Pin; Khumsupan, Darin; Lin, Shin Ping; Angkawijaya, Artik Elisa; Cheng, Kuan‐Chen

doi:10.1016/j.jep.2023.116980

Cited by 6 publications

(2 citation statements)

References 36 publications

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“…These five metabolites have been documented to exert anti-inflammatory and anti-metabolic disease effects. For example, gluconic acid has been found to inhibit NF-κB activity along with its downstream inflammation signaling cascade ( Hsieh et al, 2024 ). Supplementation with lysine upregulates anti-inflammation cytokines and downregulates pro-inflammation cytokines in fish ( Hu et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Zn(II)–curcumin prevents cadmium-aggravated diabetic nephropathy by regulating gut microbiota and zinc homeostasis

Sun,

Mei,

Wang

et al. 2024

Front. Pharmacol.

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Background: Diabetic nephropathy (DN) is known as the most common complication of diabetes, resulting from a complex inheritance-environment interaction without effective clinical treatments. Herein, we revealed the protective effects and mechanisms of Zn(II)-curcumin, a curcumin derivative, against streptozotocin-induced DN in rats in the presence or absence of cadmium exposure.Methods: The present study focused on investigating the therapy of Zn(II)-curcumin against cadmium-aggravated DN by regulating gut microbiota, metabolism, inflammation and zinc homeostasis based on pathological changes, TLR4/NF-κB signaling pathway, inductively coupled plasma-mass spectrometry (ICP-MS), 16S rRNA gene sequencing and gas chromatography-mass spectrometer (GC-MS).Results: We found Zn(II)-curcumin significantly mitigated the cadmium-aggravated phenotypes of diabetic nephropathy, as indicated by the remission of renal dysfunction, pathological changes, inflammation and zinc dyshomeostasis in streptozotocin-treated rats exposed to cadmium. Administration of Zn(II)-curcumin significantly alleviated the dysbiosis of gut microbiota and the changes of serum metabolite profiles in rats treated with streptozotocin in combination with cadmium. Notably, fecal microbial transplantation identified the ability of Zn(II)-curcumin to regulate renal function, inflammation and zinc homeostasis was partly dependent on the gut microbiota.Conclusion: These findings revealed that Zn(II)-curcumin alleviated cadmium-aggravated diabetic nephropathy by reshaping the gut microbiota and zinc homeostasis, which provided unique insights into the mechanisms of the treatment and prevention of diabetic nephropathy.

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Section: Discussionmentioning

confidence: 99%

Zn(II)–curcumin prevents cadmium-aggravated diabetic nephropathy by regulating gut microbiota and zinc homeostasis

Sun,

Mei,

Wang

et al. 2024

Front. Pharmacol.

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“…Studies have shown that PM 2.5 exposure can cause a variety of respiratory diseases, such as inflammation of airways and lung macrophages, pulmonary fibrosis, etc. [4][5][6]. At the same time, it may cause pyroptosis and ferroptosis, which can lead to pulmonary toxicity [7], particularly in livestock housing due to the dense Toxics 2023, 11, 981 2 of 17 and relatively enclosed animal population.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA miR-212-5p Regulates the MEK/ERK Signaling Pathway by Targeting A-Raf proto-oncogene serine/threonine-protein kinase (ARAF) to Regulate Cowshed PM2.5-Induced NR8383 Apoptosis

Sun,

Jia

et al. 2023

Toxics

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Objective: To investigate the role of miR-212-5p-targeted ARAF during the apoptosis of rat alveolar macrophages induced by cowshed PM2.5. Methods: miRNA and related target genes and pathways were predicted using the KEGG, TargetScan, and other prediction websites. NR8383 macrophages were treated with cowshed PM2.5 to establish an in vitro lung injury model in rats; meanwhile, for the assessment of cell viability, apoptosis, intracellular calcium ions, and mitochondrial membrane potential in NR8383 cells, RT-qPCR was used to detect the expression of miR-212-5p and the target gene ARAF. Results: The bioinformatic analyses showed that miR-212-5p and ARAF were involved in PM2.5-associated cellular damage. Exposure to different concentrations (0 μg/mL, 60 μg/mL, 180 μg/mL, 300 μg/mL) with different durations (0 h, 12 h, 24 h, 48 h) of cowshed PM2.5 resulted in apoptosis, increased intracellular calcium ions, and decreased mitochondrial membrane potential. The miR-212-5p mimic group showed an up-regulation of Bax and cleaved Caspase 3 expression but decreased Bcl2 expression compared to the NC group, and overexpression of ARAF up-regulated the expression of p-MEK1/2 and p-ERK1/2 and simultaneously reversed the above phenomena. Conclusions: miR-212-5p targets ARAF to affect the cowshed PM2.5-induced apoptosis through the MEK/ERK signaling pathway, providing a potential target for relevant farming industry and pathology studies.

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Evaluation of the amelioration effect of Ganoderma formosanum extract on delaying PM2.5 damage to lung macrophages

Hou,

Hsieh,

Hung

et al. 2024

Molecular Nutrition Food Res

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ScopeParticulate matter (PM) contains toxic organic matter and heavy metals that enter the entire body through blood flow and may cause mortality. Ganoderma formosanum mycelium, a valuable traditional Chinese medicine that has been used since ancient times, contains various active ingredients that can effectively impede inflammatory responses on murine alveolar macrophages induced by PM particles.Methods and ResultsAn experimental study assessing the effect of G. formosanum mycelium extract's water fraction (WA) on PM‐exposed murine alveolar macrophages using ROS measurement shows that WA reduces intracellular ROS by 12% and increases cell viability by 16% when induced by PM particles. According to RNA‐Sequencing, western blotting, and real‐time qPCR are conducted to analyze the metabolic pathway. The WA reduces the protein ratio in p‐NF‐κB/NF‐κB by 18% and decreases the expression of inflammatory genes, including IL‐1β by 38%, IL‐6 by 29%, and TNF‐α by 19%. Finally, the identification of seven types of anti‐inflammatory compounds in the WA fraction is achieved through UHPLC‐ESI‐Orbitrap‐Elite‐MS/MS analysis. These compounds include anti‐inflammatory compounds, namely thiamine, adenosine 5ʹ‐monophosphate, pipecolic acid, L‐pyroglutamic acid, acetyl‐L‐carnitine, D‐mannitol, and L‐malic acid.ConclusionsThe study suggests that the WA has the potential to alleviate the PM ‐induced damage in alveolar macrophages, demonstrating its anti‐inflammatory properties.

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Alleviation of PM2.5-induced alveolar macrophage inflammation using extract of fermented Chenopodium formosanum Koidz sprouts via regulation of NF-κB pathway

Cited by 6 publications

References 36 publications

Zn(II)–curcumin prevents cadmium-aggravated diabetic nephropathy by regulating gut microbiota and zinc homeostasis

Zn(II)–curcumin prevents cadmium-aggravated diabetic nephropathy by regulating gut microbiota and zinc homeostasis

MicroRNA miR-212-5p Regulates the MEK/ERK Signaling Pathway by Targeting A-Raf proto-oncogene serine/threonine-protein kinase (ARAF) to Regulate Cowshed PM2.5-Induced NR8383 Apoptosis

Evaluation of the amelioration effect of Ganoderma formosanum extract on delaying PM2.5 damage to lung macrophages

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