Alleviation of methyl viologen-mediated oxidative stress by Brassica juncea annexin-3 in transgenic Arabidopsis

Dalal, Ahan; Kumar, Abhay; Yadav, Deepanker; Gudla, Triveni; Viehhauser, Andrea; Dietz, Karl‐Josef; Kirti, Pulugurtha Bharadwaja

doi:10.1016/j.plantsci.2013.12.016

Cited by 25 publications

(14 citation statements)

References 69 publications

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“…Subsequent analyses confirmed that not only ATANN1, but also Brassica juncea annexins BJANN1 and BJANN3, and Nelumbo nucifera annexin NNANN1 ameliorated oxidative stress in homologous or heterologous cells and improved stress tolerance of tobacco, cotton, and Arabidopsis [ 23 , 25 – 30 ]. In some cases, overexpression of annexins resulted in multi-stress tolerance.…”

Section: Introductionmentioning

confidence: 96%

Potato Annexin STANN1 Promotes Drought Tolerance and Mitigates Light Stress in Transgenic Solanum tuberosum L. Plants

et al. 2015

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Annexins are a family of calcium- and membrane-binding proteins that are important for plant tolerance to adverse environmental conditions. Annexins function to counteract oxidative stress, maintain cell redox homeostasis, and enhance drought tolerance. In the present study, an endogenous annexin, STANN1, was overexpressed to determine whether crop yields could be improved in potato (Solanum tuberosum L.) during drought. Nine potential potato annexins were identified and their expression characterized in response to drought treatment. STANN1 mRNA was constitutively expressed at a high level and drought treatment strongly increased transcription levels. Therefore, STANN1 was selected for overexpression analysis. Under drought conditions, transgenic potato plants ectopically expressing STANN1 were more tolerant to water deficit in the root zone, preserved more water in green tissues, maintained chloroplast functions, and had higher accumulation of chlorophyll b and xanthophylls (especially zeaxanthin) than wild type (WT). Drought-induced reductions in the maximum efficiency and the electron transport rate of photosystem II (PSII), as well as the quantum yield of photosynthesis, were less pronounced in transgenic plants overexpressing STANN1 than in the WT. This conferred more efficient non-photochemical energy dissipation in the outer antennae of PSII and probably more efficient protection of reaction centers against photooxidative damage in transgenic plants under drought conditions. Consequently, these plants were able to maintain effective photosynthesis during drought, which resulted in greater productivity than WT plants despite water scarcity. Although the mechanisms underlying this stress protection are not yet clear, annexin-mediated photoprotection is probably linked to protection against light-induced oxidative stress.

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Section: Introductionmentioning

confidence: 96%

Potato Annexin STANN1 Promotes Drought Tolerance and Mitigates Light Stress in Transgenic Solanum tuberosum L. Plants

et al. 2015

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“…Ectopic expression of B. juncea annexin gene BjAnn1 in tobacco and cotton enhanced tolerance to various abiotic stresses and fungal pathogen [39][40][41] . AnnBj3 promoted oxidative stress tolerance in plants 42 .Brassica napus (genome AnAnCnCn) is an important oil crop worldwide, which was formed by recent allopolyploidy between ancestors of Brassica rapa (genome ArAr) and Brassica oleracea (genome CoCo) 43 . The production and quality of B. napus is greatly influence by adverse environmental conditions.…”

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confidence: 99%

Comprehensive analyses of the annexin (ANN) gene family in Brassica rapa, Brassica oleracea and Brassica napus reveals their roles in stress response

Liao

Xie

et al. 2020

Sci Rep

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Annexins (Ann) are a multigene, evolutionarily conserved family of calcium-dependent and phospholipid-binding proteins that play important roles in plant development and stress resistance. However, a systematic comprehensive analysis of ANN genes of Brassicaceae species (Brassica rapa, Brassica oleracea, and Brassica napus) has not yet been reported. In this study, we identified 13, 12, and 26 ANN genes in B. rapa, B. oleracea, and B. napus, respectively. About half of these genes were clustered on various chromosomes. Molecular evolutionary analysis showed that the ANN genes were highly conserved in Brassicaceae species. Transcriptome analysis showed that different group ANN members exhibited varied expression patterns in different tissues and under different (abiotic stress and hormones) treatments. Meanwhile, same group members from Arabidopsis thaliana, B. rapa, B. oleracea, and B. napus demonstrated conserved expression patterns in different tissues. The weighted gene coexpression network analysis (WGcnA) showed that BnaANN genes were induced by methyl jasmonate (MeJA) treatment and played important roles in jasmonate (JA) signaling and multiple stress response in B. napus.Annexins (ANN) are a multigene, evolutionarily conserved family of calcium (Ca 2+ )-dependent and phospholipid-binding proteins present in plants, animals, and microorganisms 1,2 . ANN contain the characteristic annexin repeat and they regulate membrane dynamics, mediate Ca 2+ sensing and signaling, link Ca 2+ dynamics to cytoskeletal responses, and mediate immune or stress responses and signaling during plant growth and development 1,3 . A typical ANN contains four annexin repeats at the C-terminal region and a highly variable N-terminal region. Each annexin repeat usually contains a characteristic type II motif for Ca 2+ binding 1,3 . The variable N-terminal region interacts with other proteins and is responsible for the functional diversity of ANN 4 .Recent studies have identified the ANN gene family in Arabidopsis thaliana (8 genes), Brassica rapa (13), Solanum lycopersicum (9), Solanum tuberosum (9), Oryza sativa (10), Triticum aestivum (25), Gossypium raimondii (14), Arachis hypogaea L. (8), Hordeum vulgare (11), Medicago truncatula (10), Populus trichocarpa (12), Vitis vinifera (14), Carica papaya (12), Glycine max (22), Cochliobolus sativus (11), Sorghum bicolor (10), Zea mays (12), Brachypodium distachyon (11), Selaginella mollendorffii (5), and Physcomitrella patens (7) via genome-wide analysis 2,5-11 .Studies have shown that ANN gene family plays a significant role in plant development and plant protection during both abiotic and biotic stresses 1,3,12,13 . In Arabidopsis, two ANN genes (AtANN1 and AtANN4) were regulated by abiotic stress, negatively regulated plant tolerance to drought, salinity, and heat stress, while AtANN8 was open Scientific RepoRtS | (2020) 10:4295 | https://doi.org/10.1038/s41598-020-59953-w www.nature.com/scientificreports www.nature.com/scientificreports/ positive regulated the plant abiotic tolerance [...

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“…It suggests that AnnAt8 helped transgenic seedlings not only in surviving the MV stress, but also in maintaining their growth under MV-induced stress. A recent report also suggests that AnnBj3 of mustard plays a role in the alleviation of MV-mediated photooxidative stress in Arabidopsis [ 53 ]. The mechanism behind the AnnAt8 mediated MV stress tolerance needs to be studied further.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of Arabidopsis AnnAt8 Alleviates Abiotic Stress in Transgenic Arabidopsis and Tobacco

Yadav

Ahmed

Shukla

et al. 2016

Plants

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Abiotic stress results in massive loss of crop productivity throughout the world. Because of our limited knowledge of the plant defense mechanisms, it is very difficult to exploit the plant genetic resources for manipulation of traits that could benefit multiple stress tolerance in plants. To achieve this, we need a deeper understanding of the plant gene regulatory mechanisms involved in stress responses. Understanding the roles of different members of plant gene families involved in different stress responses, would be a step in this direction. Arabidopsis, which served as a model system for the plant research, is also the most suitable system for the functional characterization of plant gene families. Annexin family in Arabidopsis also is one gene family which has not been fully explored. Eight annexin genes have been reported in the genome of Arabidopsis thaliana. Expression studies of different Arabidopsis annexins revealed their differential regulation under various abiotic stress conditions. AnnAt8 (At5g12380), a member of this family has been shown to exhibit ~433 and ~175 fold increase in transcript levels under NaCl and dehydration stress respectively. To characterize Annexin8 (AnnAt8) further, we have generated transgenic Arabidopsis and tobacco plants constitutively expressing AnnAt8, which were evaluated under different abiotic stress conditions. AnnAt8 overexpressing transgenic plants exhibited higher seed germination rates, better plant growth, and higher chlorophyll retention when compared to wild type plants under abiotic stress treatments. Under stress conditions transgenic plants showed comparatively higher levels of proline and lower levels of malondialdehyde compared to the wild-type plants. Real-Time PCR analyses revealed that the expression of several stress-regulated genes was altered in AnnAt8 over-expressing transgenic tobacco plants, and the enhanced tolerance exhibited by the transgenic plants can be correlated with altered expressions of these stress-regulated genes. Our findings suggest a role for AnnAt8 in enhancing abiotic stress tolerance at different stages of plant growth and development.

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Alleviation of methyl viologen-mediated oxidative stress by Brassica juncea annexin-3 in transgenic Arabidopsis

Cited by 25 publications

References 69 publications

Potato Annexin STANN1 Promotes Drought Tolerance and Mitigates Light Stress in Transgenic Solanum tuberosum L. Plants

Potato Annexin STANN1 Promotes Drought Tolerance and Mitigates Light Stress in Transgenic Solanum tuberosum L. Plants

Comprehensive analyses of the annexin (ANN) gene family in Brassica rapa, Brassica oleracea and Brassica napus reveals their roles in stress response

Overexpression of Arabidopsis AnnAt8 Alleviates Abiotic Stress in Transgenic Arabidopsis and Tobacco

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