Alleviating Effects of Active Fraction of <i>Euonymus alatus</i> Abundant in Flavonoids on Diabetic Mice

Fang, Xiankang; Gao, Yuan; Yang, Haiyan; Lang, Su-Mei; Wang, Qiujuan; Yu, Boyang; Zhu, Daling

doi:10.1142/s0192415x08005643

Cited by 20 publications

(11 citation statements)

References 24 publications

(23 reference statements)

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“…According to the literatures, the constituents of Tangzhiqing granules could improve diabetes and its complications [21–25]. The water-extraction of herba lycopi and radix astragali could improve the clinical symptoms for the DN rats which were caused by STZ.…”

Section: Discussionmentioning

confidence: 99%

Tangzhiqing Granules Alleviate Podocyte Epithelial‐Mesenchymal Transition in Kidney of Diabetic Rats

Wang

Liu

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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This study discussed the effect of Tangzhiqing granules on podocyte epithelial-mesenchymal transition in kidney of diabetic rats. The diabetic rats were divided randomly into five groups: DM group treated with vehicle, Tangzhiqing granules low-dose treatment group, Tangzhiqing granules middle-dose treatment group, and Tangzhiqing granules high-dose treatment group. Eight Wistar rats used as control group were given saline solution. The intervention was all intragastric administration for 8 weeks. At the end of the 8 weeks, biochemical parameters and kidney weight/body weight ratio were measured. The kidney tissues were observed under light microscope and transmission electron microscopy. To search for the underlying mechanism, we examined the epithelial-to-mesenchymal transition (EMT) related molecular markers and TGF-β/smad signaling pathway key proteins expression. The results showed that Tangzhiqing granules relieved the structural damage and functional changes of diabetic kidneys. Kidney podocyte EMT related molecular markers nephrin and CD2AP expression were increased, when desmin and α-SMA levels were decreased by Tangzhiqing granules in diabetic rats. Further TGF-β/smad signaling pathway key proteins TGF-β1 and p-smad2/3 levels were decreased in diabetic rats after treatment with Tangzhiqing granules. These findings suggest that Tangzhiqing granules may protect the podocytes of diabetic nephropathy rats via alleviating podocyte EMT and likely activating TGFβ/smad signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Tangzhiqing Granules Alleviate Podocyte Epithelial‐Mesenchymal Transition in Kidney of Diabetic Rats

Wang

Liu

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…Diabetes mellitus is a metabolic disorder characterized by increased levels of blood glucose [Fang et al, 2008]. The development of overt hyperglycemia in diabetes largely correlates with the increased rates of HPG in which GP plays an important role.…”

Section: Discussionmentioning

confidence: 99%

Anti‐diabetic effect of standardized extract of Potentilla discolor Bunge and identification of its active components

Yang¹,

Chen²,

Zhang³

et al. 2009

Drug Development Research

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Potentilla discolor Bunge, a Chinese folk medicine, has been used for the treatment of diabetes for many years. Unfortunately, little information is available on its bioactive components and mechanism of action. We report the anti-diabetic effect of P. discolor on normal and alloxan-induced diabetic mice, as well as the possible constituents and mechanism responsible for this activity. We found that the standardized extract of P. discolor (EPD) had little effect on the glucose levels of normal mice, while a dose-dependent hypoglycemic effect was observed in diabetic mice. Glucose tolerance test data indicated that there was a significantly higher rate of glucose disposal after EPD treatment. Phytochemical characterization indicated that the major components of EPD were triterpenes and flavonoids that could inhibit the activity of glycogen phosphorylase (GP) in vitro. Tormentic acid, asiatic acid, and potengriffioside A exhibited inhibitory effects on GP (IC 50 5 90.6, 65.4, and 85.9 mM, respectively). The inhibition on GP may be one molecular mechanism through which EPD ameliorates hyperglycemia. These results suggest P. discolor extract may offer an alternate treatment for diabetes. Drug Dev Res 71: 127-132, 2010.

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“…Reduced NO release in diabetes mellitus damages the dilation function of arterioles. (24,25) Chinese medicines have been found to have the ability to prevent and treat diabetic nephropathy in the early stages; (26)(27)(28)(29) this may be due to the fact that they are often made of diverse effective components, which are able to act on different targets of diabetic nephropathy. The compound preparation Xiaokening is composed of extractions of rhubarb, radix aconiti lateralis preparata and crude radix astragali.…”

Section: Discussionmentioning

confidence: 99%

Xiaokening stimulates endothelial nitric oxide release in diabetic rats

Liu

Wei

et al. 2015

smedj

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INTRODUCTIONDiabetes mellitus is a group of metabolic diseases that is characterised by high blood sugar levels. This occurs either because the body does not produce enough insulin or because cells do not respond to the insulin that is produced. In the past few decades, many studies have shown a clear worldwide increase in the incidence of both type 1 and type 2 diabetes mellitus.(1) Without proper treatment, diabetes mellitus can cause many long-term complications, including cardiovascular disease, chronic renal failure, blindness and nerve damage. Microangiopathy, which is caused by vascular endothelial cell injury and dysfunction, is a fundamental process in diabetes mellitus complications.(2-4) Drug interventions are needed to treat these microvascular complications.Reactive oxygen species (ROS) play a crucial role in the pathogenesis of some diseases (e.g. cancer, cardiovascular disease, diabetes mellitus and infl ammation).(5) The effect of the increased production of free radicals in diabetes mellitus is devastating and well documented.(6-8) Streptozotocin (STZ)-induced diabetic rats are one of the animal models for type 1 diabetes mellitus.(9) In type 1 diabetes mellitus, destruction of the β-cells of the islets of Langerhans causes degranulation or reduction of insulin secretion, but not the total absence of insulin in blood.(10) The cytotoxic action of STZ is associated with the generation of ROS, which causes oxidative tissue damage.(11) There is considerable evidence of the role of altered antioxidant defences (including both nonenzymatic and enzymatic antioxidant systems) and free radicals in the aetiology of diabetes mellitus. (12,13) Many studies have reported that the use of traditional Chinese medicines not only helps to lower serum glucose, but also helps to inhibit the development of diabetic complications. ( 14,15) Our previous research, which was conducted on umbilical vein endothelial cells (ECV-304), found that Xiaokening effectively reduces cellular injury and proliferation inhibition resulting from high glucose serum levels. (16,17) In other words, Xiaokening was found to have protective effects for endothelial cells and the ability to improve cell propagation. These fi ndings indicate that Xiaokening has a positive effect on endothelial cells in vitro. However, there is little information on the effect of Xiaokening on endothelial cells within vessels. As the effect of Xiaokening on diabetic arterioles has not been reported, the aim of the present study was to explore its effect on the mesenteric arteriolar endothelial cell function of diabetic rats and the underlying mechanism. METHODSSTZ and 2,3-diaminonaphthalene (DAN) were purchased from Sigma Chemical Company, MO, USA. The STZ was dissolved in a solution of cold citrate buffer (0.1 M, pH 4.5). The DAN was dissolved in methanol. The STZ and DAN solutions used in the present study were freshly dissolved and shielded from light. Antibody for endothelial nitric oxide synthase (eNOS) was purchased from Santa Cruz Biotechnology, TX, USA....

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Alleviating Effects of Active Fraction of Euonymus alatus Abundant in Flavonoids on Diabetic Mice

Cited by 20 publications

References 24 publications

Tangzhiqing Granules Alleviate Podocyte Epithelial‐Mesenchymal Transition in Kidney of Diabetic Rats

Tangzhiqing Granules Alleviate Podocyte Epithelial‐Mesenchymal Transition in Kidney of Diabetic Rats

Anti‐diabetic effect of standardized extract of Potentilla discolor Bunge and identification of its active components

Xiaokening stimulates endothelial nitric oxide release in diabetic rats

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