Alleviating Effect of High Protein Diet on the Toxic Effect of Organophospho~C ompounds on the Growth of Rats

Abstract

Elevation of protein in the diet from 19 to 59 per cent ,and kept isocaloric significantly improved the growth over a period of 20 days of male albino rats exposed to the toxic stress of DFP, EPN and Malathion.Marked changes in the metabolism and pharmacotbxicological activity of drugs and xenobiotics have been reported due to alteration of the nutritionalfactorsl. Dietary protein increases2 the concentration of rnicrosomal drug metabolising enzymes mainly localised in the liver ~nicrosomes which readily metabolise and detoxify' any xenobiotic. However, for a chemical that gets bioactivated or biotoxicated the test animal is exposed to the stress of a more toxic metabo!ite for a longer period when on High Protein Diet (HPD).The present study deals with the effect of HPD on the growth rate of albino rats under toxicity of organophosphorus (OP) compounds like diisopropylphosphorofluoridate (DFP), o-ethyl o-(Cnitrophenyl) phenyl phosphonothioate (EPN) and Malathion [o, o-dimethyl S(1, 2 -dicarbethoxy ethyl) phosphorodithioate]. Apart from DFP which undergoes direct detoxication in a living system and is a structural analogue of nerve gas Sarin (Methyl isopropyl phosphonofluoridate), EPN and Malathion undergoes biotoxication.The aim was to find out whether HPD could be used as a prophylactic against nerve gas poisoning which are of organophosphorus origin4. Male albino rats of wistar strain (body wt. 130 f 15 g) were divided into eight groups of eight animals each. Groups A and B were controls being given Standard Diet (SD) containing 19 per -----

Comparative Evaluation of the Allevfating Effed of Sbadard, Low Proteii and High Protein Diets on the To& Edtect of Organophmphorus Compounds on the Gmwth of Rats

“…It can be seen from groups I, I1 and 111 that all the three diets SD, LPD and HPD are growth promoting as there is an increase in body weights recording positive nitrogen balance. Elevations in growth rate as compared to SD control (1) can be seen in groups 111, VI and IX. These are the If these diets are to 'be assessed with respect to their suitability in combating toxicity of at least DFP, which is structural analogue of nerve gaq sarin (methyl isopropyl phosphonofluoridate) it' may be concluded that HPD is best.…”

“…The effects of various diets either separately or in combination with OP compounds on growth rate of rats have been compared with untreated SD control (1).…”