“…Initially, the addition of hydrophobicity scales to the prediction of secondary structures gave better results [79,80]. An impressive number of methods were proposed, such as MEMSAT [81,82], HTP [83], DAS [83], SOSUI [84], HMMTOP [85,86], TMHMM 1.0 [87], PRED-TMR [88], OCTOPUS [89], TOPCONS [90,91], MINNOU [92], SVMtm [93], TUPS [94], Localizome [95], MemBrain [96], AllesTM [97], TMPSS [98], and TMbed [99]. The most recent approaches also take into account other features, such as the regions of the protein that actually face the membrane, the cytosolic or extracellular sides, and the motifs responsible for the interactions [97,[100][101][102].…”