Allergy to food odors

Horesh, Arthur J.

doi:10.1016/s0021-8707(43)90669-0

Cited by 14 publications

(2 citation statements)

References 3 publications

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“…Since the first case report of buckwheat allergy in 1909, 1) buckwheat has been recognized as one of most important food allergens manifested by severe and dangerous symptoms induced by ingestion or inhalation of a small amount of the flour or food products. [2][3][4] Buckwheat allergy is a type I immune response, i.e., an immediate hypersensitivity reaction (IHR) that includes anaphylaxis mediated by a specific IgE antibody. In addition, buckwheat allergy is rarely outgrown, unlike allergy to cow's milk and hen's eggs.…”

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confidence: 99%

Immunological Characterization and Mutational Analysis of the Recombinant Protein BWp16, a Major Allergen in Buckwheat

Satoh

Koyano

Takagi

et al. 2008

Biological & Pharmaceutical Bulletin

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Buckwheat allergy is one of the most critical diseases manifested by severe and dangerous symptoms in Japan and other countries. We previously isolated the cDNA encoding protein BWp16, a member of the 2S albumin family with a conserved motif of 8 cysteine (Cys) residues. Comparison of the deduced amino acid sequences of BWp16 and related proteins in the 2S albumin family showed similarities between BWp16 and BW 8-kDa from buckwheat, Ara h 6 from peanuts and Ric c 1 from castor bean. Purified recombinant BWp16 (rBWp16) expressed in Escherichia coli was recognized by Ͼ80% of sera from patients with positive for IgE binding to buckwheat. Mutational analysis of rBWp16 revealed that 7 out of 10 mutants in the Cys residues showed weaker IgE binding to patient's serum than wild-type rBWp16 (rBWp16 WT). Mutations of Cys65 and Cys66 in rBWp16 decreased the pepsin digestibility of the protein, and an ELISA inhibition assay revealed a weaker inhibitory effect of rBWp16 C65S than that of rBWp16 WT. These results suggest that the Cys residues, especially Cys65, are involved in the allergenicity of rBWp16. Our findings provide new evidence for the role of Cys residues in 2S albumin family proteins and open the door to the production of hypoallergens and application to safe diagnostic methods and allergen-specific immunotherapy of buckwheat allergy.

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Immunological Characterization and Mutational Analysis of the Recombinant Protein BWp16, a Major Allergen in Buckwheat

Satoh

Koyano

Takagi

et al. 2008

Biological & Pharmaceutical Bulletin

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“…Buckwheat-flour products are also becoming popular in Western countries as a healthy food with a high protein content. However, some people exhibit hypersensitivity mediated by an antibody IgE specific to buckwheat proteins (Wieslander, 1996;Horesh, 1972). Ingestion of a small amount of buckwheat can cause immediate hypersensitivity reactions, termed anaphylaxis.…”

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confidence: 99%

Purification, crystallization and preliminary X-ray analysis of a deletion mutant of a major buckwheat allergen

et al. 2009

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A 16 kDa buckwheat protein (BWp16) is a major allergen responsible for immediate hypersensitivity reactions including anaphylaxis. A deletion mutant of BWp16 (rBWp16ÁN) was overproduced and purified and was shown to be immunologically active. A three-wavelength MAD data set was collected from a crystal of selenomethionine-labelled rBWp16ÁN. The crystal belonged to the triclinic space group P1, with unit-cell parameters a = 28.39, b = 31.54, c = 32.20 Å , = 111.92, = 108.91, = 98.74 . One monomer was expected to be present in the asymmetric unit based on the calculated Matthews coefficient of 1.76 Å 3 Da À1 .

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Inhalant Allergens in Management of Atopic Dermatitis

Tuft¹

1955

Arch Pediatr Adolesc Med

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Allergy to food odors

Cited by 14 publications

References 3 publications

Immunological Characterization and Mutational Analysis of the Recombinant Protein BWp16, a Major Allergen in Buckwheat

Immunological Characterization and Mutational Analysis of the Recombinant Protein BWp16, a Major Allergen in Buckwheat

Purification, crystallization and preliminary X-ray analysis of a deletion mutant of a major buckwheat allergen

Inhalant Allergens in Management of Atopic Dermatitis

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