Allergic fungal rhinosinusitis: detection of fungal DNA in sinus aspirate using polymerase chain reaction

Elmorsy, Shawky; Khafagy, Yasser; El-Naggar, M.M.; Beih, A A

doi:10.1017/s0022215109991204

Cited by 15 publications

(8 citation statements)

References 33 publications

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“…Molecular identification based on PCR and sequencing of the internal transcribed spacer (ITS) and D1/D2 regions of rDNA is recommended for accurate identification . Direct detection of Bipolaris DNA by PCR has been reported . As with all fungi in this class, the Fontana–Masson stain is helpful for diagnosis .…”

Section: Black Fungal Species With Clinical Relevancementioning

confidence: 99%

ESCMID and ECMM joint clinical guidelines for the diagnosis and management of systemic phaeohyphomycosis: diseases caused by black fungi

Chowdhary

Meis

Guarro

et al. 2014

Clinical Microbiology and Infection

285

253

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The aetiological agents of many invasive fungal infections are saprobes and opportunistic pathogens. Some of these fungi are darkly pigmented due to melanin production and traditionally have been named 'dematiaceous'. The melanized fungi cause a wide array of clinical syndromes ranging from superficial to deep-seated infections. Diagnosis relies on histopathological examination of clinical specimens and on examination of cultures. Sequencing is recommended for accurate species identification, especially for unusual or newly described pathogens. In cases of mycetoma and chromoblastomycosis, pathognomonic histological findings are useful and the Fontana-Masson stain, specific for melanin, usually confirms the diagnosis. There are no standardized therapies but voriconazole, posaconazole and itraconazole demonstrate the most consistent in vitro activity against this group of fungi. Oral itraconazole has been considered the drug of choice, given the extensive clinical experience with this drug. However, voriconazole may presumably be superior for central nervous system infections because of its ability to achieve good levels in the cerebrospinal fluid. Posaconazole is a well-tolerated alternative drug, backed by less clinical experience but with excellent salvage treatment results after failure of other antifungals. Amphotericin B has been useful as alternative therapy in some cases. Combination antifungal therapy is recommended for cerebral abscesses when surgery is not possible and for disseminated infections in immunocompromised patients.

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Section: Black Fungal Species With Clinical Relevancementioning

confidence: 99%

ESCMID and ECMM joint clinical guidelines for the diagnosis and management of systemic phaeohyphomycosis: diseases caused by black fungi

Chowdhary

Meis

Guarro

et al. 2014

Clinical Microbiology and Infection

285

253

View full text Add to dashboard Cite

show abstract

“…Molecular identification can be done using ITS sequencing. A specific polymerase chain reaction (PCR) has been developed for the direct detection of Bipolaris species (Shin et al 2003;El-Morsy et al 2010). …”

Section: Bipolaris (Pleosporales: Pleosporaceae)mentioning

confidence: 99%

Black Molds and Melanized Yeasts Pathogenic to Humans

Chowdhary

Perfect

Hoog

2014

Cold Spring Harb Perspect Med

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“…The species present in the nasal brushings were similar to species found by Q-PCR testing of dust samples in their homes. In another study of CRS, fungal DNA was present in tissue specimens taken from patients with polyploid CRS who underwent surgery [ 30 ]. Two PCR primer sets were utilized; one was panfungal and the other specific for Alternaria .…”

Section: Chronic Rhinosinusitis (Crs)mentioning

confidence: 99%

“…In surgical specimens from healthy controls, the panfungal DNA was positive in 10 of 15 cases but all were negative for the Alternaria DNA. Studies have also shown that pre-digestion of tissue slides with trypsin before staining dramatically improves identification of fungi by immunofluorescence as does as PCR-DNA analysis [ 30 , 31 ].…”

Section: Chronic Rhinosinusitis (Crs)mentioning

confidence: 99%

Chronic Illness Associated with Mold and Mycotoxins: Is Naso-Sinus Fungal Biofilm the Culprit?

Brewer

Thrasher²,

Hooper³

2013

Toxins

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It has recently been demonstrated that patients who develop chronic illness after prior exposure to water damaged buildings (WDB) and mold have the presence of mycotoxins, which can be detected in the urine. We hypothesized that the mold may be harbored internally and continue to release and/or produce mycotoxins which contribute to ongoing chronic illness. The sinuses are the most likely candidate as a site for the internal mold and mycotoxin production. In this paper, we review the literature supporting this concept.

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Allergic fungal rhinosinusitis: detection of fungal DNA in sinus aspirate using polymerase chain reaction

Abstract: Detection of fungal DNA in nasal aspirate by polymerase chain reaction was superior to fungal cultures as a method of detecting fungal growth. In allergic fungal rhinosinusitis, fungal growth is not always accompanied by an allergic reaction.

Cited by 15 publications

References 33 publications

ESCMID and ECMM joint clinical guidelines for the diagnosis and management of systemic phaeohyphomycosis: diseases caused by black fungi

ESCMID and ECMM joint clinical guidelines for the diagnosis and management of systemic phaeohyphomycosis: diseases caused by black fungi

Black Molds and Melanized Yeasts Pathogenic to Humans

Chronic Illness Associated with Mold and Mycotoxins: Is Naso-Sinus Fungal Biofilm the Culprit?

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