Allergens in Hymenoptera Venom

Hoffman, Donald R.; Jacobson, Robert S.; Zerboni, R.

doi:10.1159/000234394

Cited by 44 publications

(17 citation statements)

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“…13 It is known that Sol i I had similar structure to phospholipase allergen in wasps. 14 In this study 5 subjects had bee venom-induced anaphylaxis with positive sIgE levels. In ELISA inhibition tests with serum from group A, significant inhibitions were noted with additions of P chinensis antigen, whereas a lesser degree of inhibition was noted with addition of yellow jacket antigen in a dose-dependent manner, suggesting that cross-reactivity could be greater if higher concentrations of yellow jacket antigen (>1 mg/mL) were added.…”

Section: Figmentioning

confidence: 88%

Anaphylaxis caused by the new ant, Pachycondyla chinensis : Demonstration of specific IgE and IgE-binding components

Kim¹,

Park²,

Kim³

et al. 2001

Journal of Allergy and Clinical Immunology

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Background: There have been no reports dealing with the pathogenic mechanism and IgE-binding components in patients with anaphylaxis caused by a sting from Pachycondyla chinensis. Objectives: This study was conducted to observe the clinical features of patients with P chinensis-induced anaphylaxis. The roles of specific (s) IgE and sIgG4 antibodies were evaluated, and IgE-binding components were identified. Methods: Seven patients with P chinensis-induced anaphylaxis and 15 unexposed control subjects were enrolled. P chinensis ants were collected at the patients' homes, and venom was prepared as P chinensis extract. Five patients complained of bee venom-induced anaphylaxis and had positive sIgE levels to yellow jacket venom, wasp venom, or both as well. Serum sIgE and sIgG4 were detected by means of ELISA. To identify IgEbinding components within P chinensis extracts, 12% SDS-PAGE with immunoblot analysis was applied. Results: All patients had positive skin prick test responses to P chinensis antigen and positive sIgE levels. Five (71%) patients had positive sIgG4 levels. Eight IgE-binding components (58, 46, 3l, 29, 27, 25, 22, and 12 kd) were noted, and the component at 12 kd was the most frequently found allergen (85%). IgE ELISA inhibition tests were performed on 2 groups of sera: one from patients with anaphylaxis induced by both P chinensis and bee venom (group A) and the other from patients with anaphylaxis induced by P chinensis venom alone without bee venom allergy (group B). ELISA inhibition tests with serum from group A showed significant inhibitions with addition of P chinensis extract, partial inhibitions with yellow jacket antigen, and minimal inhibitions with wasp or imported fire ant antigens. However, ELISA inhibition tests with serum from group B showed significant inhibitions with P chinensis antigen but no inhibition with wasp, yellow jacket, or imported fire ant antigens. Conclusions

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Section: Figmentioning

confidence: 88%

Anaphylaxis caused by the new ant, Pachycondyla chinensis : Demonstration of specific IgE and IgE-binding components

Kim¹,

Park²,

Kim³

et al. 2001

Journal of Allergy and Clinical Immunology

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“…The cross-reactivity of these antigens between different species of hymenoptera has been studied extensively [19,24,25,26,27], especially by Hoffman et al [26]. However, only a single study [19] has examined this issue in regard to VO.…”

Section: Discussionmentioning

confidence: 99%

Immunotherapy with Commercial Venoms Is Efficacious for Anaphylactic Reactions to <b><i>Vespa orientalis</i></b> Stings

Goldberg

Shefler²,

Panasoff

et al. 2013

Int Arch Allergy Immunol

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Background:Vespa orientalis (VO) stings occasionally induce anaphylaxis. In the absence of commercial VO venom, allergists use commercial venoms for immunotherapy, despite having no indication regarding efficacy. We attempted to examine the effectiveness of immunotherapy with commercial venoms in patients with VO allergy and to identify the venom accountable for this effect. Methods: Patients who unequivocally identified VO as the culprit insect were administered venom immunotherapy with the commercial venoms available in Israel to which they had positive skin tests. Patients were also skin tested with VO venom sac extracts and, after reaching the maintenance dose, were sting challenged by a live insect. The allergenic components in the venom were determined by immunoblotting. Results: Twelve patients were recruited and, based on their skin test results, all were treated with yellow jacket (YJ) venom, either alone or combined with other venoms. All 8 patients who were sting challenged by VO demonstrated positive skin test responses to VO venom. Six of the stung patients tolerated the sting challenge uneventfully. Two patients developed minimal transient symptoms that resolved spontaneously. SDS-PAGE with patient sera suggested cross-reactivity between VO and YJ venoms at molecular weights of 39–42 kDa. Using phospholipases, antigen 5 and hyaluronidase derived from several Vespa, Dolichovespula and Vespula species, hyaluronidase is possibly accountable for inducing the allergic reaction. Conclusion: In the absence of commercial VO venom the practice of treating patients allergic to this insect with available commercial venoms seems to be efficacious and YJ venom is probably responsible for this effect.

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“…Los alergenos de la avispa, Ves m 1, y el avispón, Dol m 1, son fosfolipasas A,B y el alergeno de la abeja Api m 1 es una fosfolipasa A, (66,67). Dol m 1 y Ves m 1 poseen en la región entre los aa 99 a 220, una identidad del 31,2% con las secuencias de las triacilglicerol lipasas pancreáticas de los mamiferos.…”

Section: Fosfolipasasunclassified

“…Dol m 1 y Ves m 1 poseen en la región entre los aa 99 a 220, una identidad del 31,2% con las secuencias de las triacilglicerol lipasas pancreáticas de los mamiferos. Sus secuencias de aa también tienen similitud con proteínas que unen calcio, aunque esta actividad no se les conoce (66). Entre la fosfolipasa A, de las abejas y la fosfolipasa A,B de las avispas existe poca similitud en sus secuencias de aa y no tienen alergenicidad cruzada (66).…”

Section: Fosfolipasasunclassified

“…Sus secuencias de aa también tienen similitud con proteínas que unen calcio, aunque esta actividad no se les conoce (66). Entre la fosfolipasa A, de las abejas y la fosfolipasa A,B de las avispas existe poca similitud en sus secuencias de aa y no tienen alergenicidad cruzada (66). El sitio catalítico de Api m 1 es similar al de la fosfolipasa A, de la cobra china (Naja naja atra) (68,69).…”

Section: Fosfolipasasunclassified

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Alergenos: relación entre función biológica y alergenicidad

1998

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ResumenLos alergenos se encuentran en muy diversas fuentes, pero, tienen la particularidad de inducir la producción de inmunoglobulina E (IgE) y provocar alergia. No se conoce una característica molecular biológica común entre los alergenos que explique su capacidad alergénica. En los últimos años, se han demostrado o inferido diferentes funciones biológicas en los alergenos, generando nuevas hipótesis sobre el papel de dichas funciones en la actividad alergénica. Con el fin de identificar qué funciones biológicas se han demostrado o inferido en los alergenos y analizar su posible influencia en el papel alergénico, se revisaron aproximadamente doscientos alergenos teniendo como base los ya caracterizados por el Comité de Nomenclatura de Alergenos de la OMS, como también otros menos caracterizados pero con algunas propiedades fisico~~liímicas conocidas. Los alergenos se agruparon de acuerdo con la actividad biológica y se tabularon con su fuente de origen, peso molecular (PM), punto isoeléctrico (pl), frecuencia de reactividad en la población alérgica, potenciales sitios de glicosilación y formación de enlaces disulfuro. Se identificaron 88 alergenos en los que se ha informado alguna actividad biológica. El agrupamiento de estos según la actividad produjo los siguientes resultados: enzimática (47,2%), inhibición de enzimas (11,3%), transporte (18,1%), regulación de la actividad celular (15,9%) y otras actividades como la de conferir resistencia a enfermedades en plantas y citólisis (7,9%). En muchos de estos alergenos, la actividad biológica ha sido inferida por su homología estructural con proteínas de función conocida, pero los experimentos que corroboren dicha función no se han realizado. La mayoría de estos alergenos tienen en común PM<60 kDa, p1<7,0 y escasa formación de oligómeros. Nuestro análisis sugiere que la capacidad de inducir alergia no está determinada por ninguna de las funciones biológicas descritas. Aunque la actividad de cisterna-proteasa y serina-proteasa de algunos de los alergenos de los ácaros domésticos han sido señaladas como determinantes en el papel alergénico en dichos alergenos, los datos experimentales no son concluyentes y no permiten atribuir la alergenicidad a la función enzimática. Allergens: the relationship between biological function and allergenicityAllergens can be found from many diverse sources but they have the particular quality of being able to induce immunoglobulin E (IgE) production and to provoke allergy. A common biological molecular characteristic which can explain their allergenic capacity is not known amongst the allergens. In the last few years allergens' different biological functions have been demonstrated or inferred, generating a new hypothesis about the role of the said functions in allergenic activity. To identify which biological functions have Instituto de Investigaciones Inmunológicas, Universidad de Cartagena Recibido para su publicación: enero 15 de 1998.

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Allergens in Hymenoptera Venom

Cited by 44 publications

References 16 publications

Anaphylaxis caused by the new ant, Pachycondyla chinensis : Demonstration of specific IgE and IgE-binding components

Anaphylaxis caused by the new ant, Pachycondyla chinensis : Demonstration of specific IgE and IgE-binding components

Immunotherapy with Commercial Venoms Is Efficacious for Anaphylactic Reactions to <b><i>Vespa orientalis</i></b> Stings

Alergenos: relación entre función biológica y alergenicidad

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