Allergenicity potential of red kidney bean (Phaseolus vulgaris L.) proteins in orally treated BALB/c mice and passively sensitized RBL-2H3 cells

Kumar, Sandeep; Sharma, Akanksha; Verma, Alok; Chaudhari, Bhushan P.; Das, Mukul; Jain, Swatantra Kumar; Dwivedi, Premendra D.

doi:10.1016/j.cellimm.2013.07.001

Cited by 14 publications

(13 citation statements)

References 39 publications

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“…The development of pigeon peainduced anaphylaxis in EC and orally-sensitized mice was in agreement with results from investigators who reported that EC (using OVA) (Bartnikas et al 2013) and orally (using red kidney bean) (Kumar et al 2013) sensitized mice followed by oral and intraperitoneal challenge, respectively, resulted in systemic anaphylaxis with an increase in anaphylactic score and decreased body temperature.…”

Section: Discussionsupporting

confidence: 91%

“…GATA-3 effector T H 2 cell levels are found to be increased after food allergen exposure followed to a lesser extent by those of T-bet þ T H 1 cells (Lu et al 2011). As a result, the GATA-3:T-bet ratio is an important index when looking for any T H 2-type shifts during allergic diseases (Kumar et al 2013;Sharma et al 2014). In the present study, elevated levels of GATA-3 and reduced levels of T-bet were found in the skin and intestine of the treated mice, again indicating involvement of T H 2-mediated allergic reactions in the EC and orally-sensitized mice.…”

Section: Discussionmentioning

confidence: 99%

“…In the oral treatment regimens (Kumar et al 2013), mice in Group O1 received daily 100 ll PBS, while those in Groups O2 and O3 received, respectively, 100 lg pigeon pea or peanut CPE (in 100 ll PBS) for up to 42 days by gavage. Blood samples were drawn on Days 15, 43 and 59 to enable measures of serum IgE, and IgG 1 .…”

Section: Ec and Oral Treatmentsmentioning

confidence: 99%

“…Several reports have reflected the role of legumes, including peanuts, chickpeas, red kidney beans, green gram and broad beans in provoking anaphylactic symptoms in Balb/c mice (Misra et al 2010;Verma et al 2012;Kumar et al 2013). Food-dependent exerciseinduced anaphylaxis to chickpea and soya bean allergens have been reported in sensitized individuals (Orhan & Karakas 2008;Adachi et al 2009;Ciccarelli et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Cutaneous exposure to clinically-relevant pigeon pea (Cajanus cajan) proteins promote T_H2-dependent sensitization and IgE-mediated anaphylaxis in Balb/c mice

Gupta

Kumar

Gupta

et al. 2016

Journal of Immunotoxicology

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Epicutaneous (EC) sensitization to food allergens may occur when the skin has been lightly damaged. The study here tested whether cutaneous exposure to pigeon pea protein(s) may cause allergic sensitization. BALB/c mice were either orally gavaged or epicutaneously sensitized by repeated application of pigeon pea crude protein extract (CPE) on undamaged areas of skin without any adjuvant; afterwards, both groups were orally challenged with the pigeon pea CPE. Anaphylactic symptoms along with measures of body temperature, MCPT-1, TSLP, pigeon pea-specific IgE and IgG 1 , myeloperoxidase (MPO) activity, T H 2 cytokines, T H 2 transcription factors (TFs) and filaggrin expression were determined. Mast cell staining, eosinophil levels and histopathological analysis of the skin and intestines were also performed. In the epicutaneously-sensitized mice, elevated levels of specific IgE and IgG 1 , as well as of MCPT-1, TSLP, T H 2 cytokines and TFs, higher anaphylactic scores and histological changes in the skin and intestine were indicative of sensitization ability via both routes in the pigeon pea CPE-treated hosts. Elevated levels of mast cells were observed in both the skin and intestine; increased levels of eosinophils and MPO activity were noted only in the skin. Decreased levels of filaggrin in skin may have played a key role in the skin barrier dysfunction, increasing the chances of sensitization. Therefore, the experimental data support the hypothesis that in addition to oral exposure, skin exposure to food allergens can promote T H 2-dependent sensitization, IgE-mediated anaphylaxis and intestinal changes after oral challenge. Based on this, an avoidance of cutaneous exposures to allergens might prevent development of food anaphylaxis. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Ec and Oral Treatmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cutaneous exposure to clinically-relevant pigeon pea (Cajanus cajan) proteins promote T_H2-dependent sensitization and IgE-mediated anaphylaxis in Balb/c mice

Gupta

Kumar

Gupta

et al. 2016

Journal of Immunotoxicology

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“…Owing to the relative resistance of the allergen protein to proteolytic degradation, primary epitope regions of undigested lectins can bind to the epithelium in the gut and result in the nutrient deficiencies via damage to enterocyte brush border and/or interaction with digestive enzymes, and it can pass the epithelial barrier to stimulate the production of antibodies and/or induce T cell immunity [11,12]. Then, nausea, emesis, diarrhea and other disrupting digestion clinical symptoms would be induced by IgE mediated immediate-type hypersensitivity reactions and non IgE mediated reactions (cell-mediated immunity) [2,7,13]. However, little information on legume lectin epitopes is hitherto available [11].…”

Section: Introductionmentioning

confidence: 99%

In Silico Identification and in Vitro Analysis of B and T-Cell Epitopes of the Black Turtle Bean (Phaseolus Vulgaris L.) Lectin

Zhao

Elfalleh

et al. 2018

Cell Physiol Biochem

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Background/Aims: The incidence of lectin allergic disease is increasing in recent decades, and definitive treatment is still lacking. Identification of B and T-cell epitopes of allergen will be useful in understanding the allergen antibody responses as well as aiding in the development of new diagnostics and therapy regimens for lectin poisoning. In the current study, we mainly addressed these questions. Methods: Three-dimensional structure of the lectin from black turtle bean (Phaseolus vulgaris L.) was modeled using the structural template of Phytohemagglutinin from P. vulgaris (PHA-E, PDB ID: 3wcs.1.A) with high identity. The B and T-cell epitopes were screened and identified by immunoinformatics and subsequently validated by ELISA, lymphocyte proliferation and cytokine profile analyses. Results: Seven potential B-cell epitopes (B1 to B7) were identified by sequence and structure based methods, while three T-cell epitopes (T1 to T3) were identified by the predictions of binding score and inhibitory concentration. The epitope peptides were synthesized. Significant IgE binding capability was found in B-cell epitopes (B2, B5, B6 and B7) and T2 (a cryptic B-cell epitope). T1 and T2 induced significant lymphoproliferation, and the release of IL-4 and IL-5 cytokine confirmed the validity of T-cell epitope prediction. Abundant hydrophobic amino acids were found in B-cell epitope and T-cell epitope regions by amino acid analysis. Positively charged amino acids, such as His residue, might be more favored for B-cell epitope. Conclusion: The present approach can be applied for the identification of epitopes in novel allergen proteins and thus for designing diagnostics and therapies in lectin allergy.

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Insight into the allergenicity of digestion-resistant epitopes in Antarctic krill (Euphausia superba) tropomyosin

Liu,

Wang,

Lin

et al. 2024

Food Bioscience

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Allergenicity potential of red kidney bean (Phaseolus vulgaris L.) proteins in orally treated BALB/c mice and passively sensitized RBL-2H3 cells

Cited by 14 publications

References 39 publications

Cutaneous exposure to clinically-relevant pigeon pea (Cajanus cajan) proteins promote T_H2-dependent sensitization and IgE-mediated anaphylaxis in Balb/c mice

Cutaneous exposure to clinically-relevant pigeon pea (Cajanus cajan) proteins promote T_H2-dependent sensitization and IgE-mediated anaphylaxis in Balb/c mice

In Silico Identification and in Vitro Analysis of B and T-Cell Epitopes of the Black Turtle Bean (Phaseolus Vulgaris L.) Lectin

Insight into the allergenicity of digestion-resistant epitopes in Antarctic krill (Euphausia superba) tropomyosin

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Allergenicity potential of red kidney bean (Phaseolus vulgaris L.) proteins in orally treated BALB/c mice and passively sensitized RBL-2H3 cells

Cited by 14 publications

References 39 publications

Cutaneous exposure to clinically-relevant pigeon pea (Cajanus cajan) proteins promote TH2-dependent sensitization and IgE-mediated anaphylaxis in Balb/c mice

Cutaneous exposure to clinically-relevant pigeon pea (Cajanus cajan) proteins promote TH2-dependent sensitization and IgE-mediated anaphylaxis in Balb/c mice

In Silico Identification and in Vitro Analysis of B and T-Cell Epitopes of the Black Turtle Bean (Phaseolus Vulgaris L.) Lectin

Insight into the allergenicity of digestion-resistant epitopes in Antarctic krill (Euphausia superba) tropomyosin

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Product

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Cutaneous exposure to clinically-relevant pigeon pea (Cajanus cajan) proteins promote T_H2-dependent sensitization and IgE-mediated anaphylaxis in Balb/c mice

Cutaneous exposure to clinically-relevant pigeon pea (Cajanus cajan) proteins promote T_H2-dependent sensitization and IgE-mediated anaphylaxis in Balb/c mice