Allergenicity and allergens of amphipods found in nori (dried laver)

Abstract

Gammaridean and caprellid amphipods, crustaceans of the order Amphipoda, inhabit laver culture platforms and, hence, are occasionally found in nori (dried laver) sheets. Amphipods mixed in nori may cause allergic reactions in sensitized patients, as is the case with other crustaceans, such as shrimp and crab, members of the order Decapoda. In this study, dried samples of amphipods (unidentified) found in nori and fresh samples of gammaridean amphipod (Gammarus sp., not accurately identified) and caprellid amphipod (Caprella equilibra) were examined for allergenicity and allergens using two species of decapods (black tiger prawn and spiny lobster) as references. When analyzed by ELISA, sera from crustacean-allergic patients reacted to extracts from amphipod samples, although less potently than to the extracts from decapods. In IgE-immunoblotting, a 37-kDa protein was found to be the major allergen in amphipods. Based on the molecular mass and the cross-reactivity with decapod tropomyosin evidenced by inhibition ELISA and inhibition immunoblotting, the 37-kDa protein was identified as amphipod tropomyosin.

New insights into seafood allergy

“…For example, contaminants such as the parasite Anisakis, if present, can cause severe allergic reactions [54]. The eight allergens from this parasite currently characterized include tropomyosin, which cross-reacts with shellfish allergens, as well as 272 Food allergy Tropomyosin [27] paramyosin and different protease inhibitors [54][55][56][57][58] ( Table 4). A recent study demonstrated that these parasites could also cause allergic sensitization among fish-processing workers [59].…”

“…The recombinant protein was subsequently produced and identified as a myosin light chain with high amino acid similarity to Bla g 8 from cockroaches. In addition, a sarcoplasmatic calcium-binding protein was identified as allergen in black tiger shrimp [25] and tropomyosin in two krill species [26] and an amphipod [27].…”

Quality Evaluation on Use of Camellia Oil as an Alternative Method in Dried Seaweed Preparation

“…The red algae genus, Porphyra yezoensis (i.e., nori), is one of the most commonly consumed seaweeds in East and Southeast Asia, and its consumption is rapidly spreading throughout the world. In Japan, approximately nine billion nori sheets are produced per year and utilized for various culinary purposes (e.g., sushi, soups, and salads) ( 7 ). Nori has been gaining a great deal of attention due to its various biological activities (e.g., anti-allergic activity, chemoprotective activity, anti-inflammatory activity, and detoxification activity) ( 8 – 11 ).…”

Potensi Rumput Laut Merah (Gracilaria Gigas) Dan Penambahan Daun Kenikir (Cosmos Caudatus) Sebagai Bahan Baku Pembuatan Nori

“…Tabel 6 menunjukkan nilai kuat tarik berkisar antara 2-12,1 N. Semakin kecil ukuran mesh akan meloloskan lebih banyak.Terjadinya kekuatan tarik dikarenakan adanya saling interaksi antara ikatan hidrogen antar molekul CH 2 OH pada struktur agar dan OH. Kuat tarik menunjukkan ketahanan nori (Motoyama et al, 2007).…”